Abstract
The destruction of Brazilian natural habitats has reduced bee populations and negative impacts of native flora pollination have been noticed. This work describes the isolation and characterization of microsatellite loci and evaluates them as molecular markers to study genetic variability of the stingless bee Plebeia remota. A microsatellite enriched genomic library was constructed and 15 primer pairs were designed for this species. The survey was conducted by analyzing 21 unrelated individuals. Genetic diversity indexes were calculated. The mean allelic richness was 6.3, the observed heterozygosity was 0.568, and the percentage of polymorphic loci was 93.33%. Also the primers were tested in cross-species amplification and showed promising results for P. droryana, P. emerina, P. lucii, P. meridionalis, P. pugnax, and P. saiqui. The microsatellite loci described here will be useful to evaluate genetic variability of stingless bees, and certainly will improve our knowledge about population dynamics especially in threatened environments.
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Acknowledgments
We thank Susy Coelho for technical assistance; Dr Anete P. de Souza for helping with the genomic library protocol; Fundação de Amparo à Pesquisa do Estado de São Paulo for PhD scholarships to FOF (2008/08546-4) and FCP and for financial support (2004/15801-0 and 2010/50597- 5), Conselho Nacional de Desenvolvimento Científico e Tecnológico for a PhD scholarship to AMTDY, and Coordenação de Aperfeiçoamento de Pessoal de Nível Superior for a master scholarship to LRS and PHPG.
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Francisco, F.O., Brito, R.M., Santiago, L.R. et al. Isolation and characterization of 15 microsatellite loci in the stingless bee Plebeia remota (Apidae: Meliponini). Conservation Genet Resour 3, 417–419 (2011). https://doi.org/10.1007/s12686-010-9369-0
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DOI: https://doi.org/10.1007/s12686-010-9369-0