Abstract
An enriched genomic library was constructed from Tetragonisca angustula, a stingless bee species widely distributed in Brazil. The library was screened using two simple-repeat oligonucleotide probes and 21 microsatellite primer pairs were designed flanking a selection of repeat sequences within positive clones. The polymorphism of the microsatellite loci was analyzed by screening a sample of 19 unrelated T. angustula workers. Fifteen out of 21 loci were shown to be polymorphic, with observed heterozygosity estimates ranging from 0.00 to 0.89. The primers were also successfully used to amplify microsatellite loci from other stingless bee species, Tetragonisca fiebrigi, Tetragonisca weyrauchi, Lestrimelitta maracaia and Schwarziana quadripunctata. The results from variability analyses suggest that the microsatellite loci isolated from T. angustula will be useful in further population studies for the species and also for other Meliponini.
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Acknowledgements
We would like to express our gratitude to Susy Coelho Oliveira for the technical support; Dr João M. F. Camargo for the identification of specimens; Dr Anete P. de Souza for protocols’ adaptation; Dr. Timothy Schaerf for the English revision; Conselho Nacional de Desenvolvimento Científico e Tecnológico for a PhD scholarship to AMTDY, Coordenação de Aperfeiçoamento de Pessoal de Nível Superior for a master degree scholarship to PHPG, and Fundação de Amparo à Pesquisa do Estado de São Paulo for PhD scholarships to FOF and FCP and for financial support (BIOTA 2004/15801-0).
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Brito, R.M., Francisco, F.O., Domingues-Yamada, A.M.T. et al. Characterization of microsatellite loci of Tetragonisca angustula (Hymenoptera, Apidae, Meliponini). Conservation Genet Resour 1, 183–187 (2009). https://doi.org/10.1007/s12686-009-9045-4
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DOI: https://doi.org/10.1007/s12686-009-9045-4