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Biohydrogen Production From Beverage Wastewater Using Selectively Enriched Mixed Culture

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Abstract

In order to select the efficient hydrogen-producers for beverage wastewater (BW), initially sucrose was chosen as a sole carbon source to enrich efficient hydrogen-producers from two different compost seeds. The enriched mixed culture (EMC) obtained from the compost of food waste (C1) provided a hydrogen yield (HY) and hydrogen production rate (HPR) of 3.76 mol/mol sucrose and 1.15 L/L-d, respectively. The EMC (C1) was further used for optimization of pH, temperature and substrate concentration from BW and showed the optimal conditions of pH 5.5, temperature 37 °C and a substrate concentration of 20 g/L, respectively. Under these conditions, the optimal HPR and HY were observed as 2.16 L/L-d and 1.30 mol/mol hexoseutilized. Ratkowsky kinetic model analysis provided the optimal temperature value of 38.5 °C. While, Monod model was used to predict the substrate concentration effects and it showed the Rm and Ks values as 3.57 L/L-d and 8.29 g/L, respectively.

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Abbreviations

BW:

Beverage wastewater (BW)

COD:

Chemical oxygen demand

CHP:

Cumulative hydrogen production

DF:

Dark fermentation

EMC:

Enriched mixed culture

HY:

Hydrogen yield

HPR:

Hydrogen production rate

SMP:

Soluble metabolic products

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Authors and Affiliations

  1. Green Energy Technology Research Group, Ton Duc Thang University, Ho Chi Minh City, Vietnam

    Periyasamy Sivagurunathan & Chiu-Yue Lin

  2. Faculty of Environment and Labour Safety, Ton Duc Thang University, Ho Chi Minh City, Vietnam

    Periyasamy Sivagurunathan

  3. Department of Environmental Engineering and Science, Feng Chia University, Taichung, 40724, Taiwan, Republic of China

    Chiu-Yue Lin

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  1. Periyasamy Sivagurunathan
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  2. Chiu-Yue Lin
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Correspondence to Periyasamy Sivagurunathan.

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Sivagurunathan, P., Lin, CY. Biohydrogen Production From Beverage Wastewater Using Selectively Enriched Mixed Culture. Waste Biomass Valor 11, 1049–1058 (2020). https://doi.org/10.1007/s12649-019-00606-z

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