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In vitro and in vivo antibacterial activity of graphene oxide-modified porous TiO2 coatings under 808-nm light irradiation

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Abstract

Bio-inertness and post-surgery infection on titanium (Ti) are the main causes of failure of biomedical implants in vivo. Near-infrared (NIR) photoactivated antibacterial therapy including photothermal and photodynamic therapies has attracted increasing attention due to the high bactericidal efficiency and little side effects. Although micro-arc oxidation (MAO) is an effective method to improve the biological activity of Ti implants, the porous TiO2 coatings prepared by MAO do not respond to near-infrared (NIR) light to kill bacteria by the photothermal and photodynamic effects. In this work, graphene oxide (GO)-modified TiO2 coatings (TiO2/GO) are prepared on Ti to improve the photothermal and photodynamic ability of the MAO coatings. The TiO2/GO coatings exhibit excellent antibacterial activity both in vitro and in vivo against Streptococcus mutans (S. mutans) under 808-nm NIR light irradiation due to the synergistic effects rendered by hyperthermia and reactive oxygen species (ROS). The NIR light-responsive antibacterial MAO coatings have large potential in combating implant-associated infections in clinical applications.

Graphic abstract

摘要 (Chinese abstract)

生物惰性和术后感染是钛植入体植入失败的主要原因。近红外光激发 (包括光热和光动力) 抗菌由于具有高效杀菌和较少的副作用引起广泛关注。微弧氧化可以有效改善钛植入体生物活性, 但制备的多孔TiO2无法被近红外光激发产生光热和光动力杀菌。针对此问题, 本文对微弧氧化制备的TiO2涂层进行了氧化石墨烯改性。改性后的涂层在近红外 808 nm激光照射下, 由于局部过热与活性氧协同作用, 展现出优良的体外和体内抗链球菌性能。这种具有近红外光响应抗菌能力的微弧氧化涂层在临床上治疗植入体相关感染有着很大潜力。

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Acknowledgements

This work was financially supported by the National Natural Science Foundation of China (No. 31870934), the Major Projects in Research and Development of Shanxi (No. 201803D421090), City University of Hong Kong Strategic Research Grant (No. 7005264), and Guangdong-Hong Kong Technology Cooperation Funding Scheme GHP/085/18SZ (No. CityU 9440230)

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Authors and Affiliations

  1. College of Biomedical Engineering, Taiyuan University of Technology, Taiyuan, 030024, China

    Mao-Zhou Chai, Mei-Wen An & Xiang-Yu Zhang

  2. Laboratory of Biomaterial Surfaces & Interfaces, Institute of New Carbon Materials, Taiyuan University of Technology, Taiyuan, 030024, China

    Xiang-Yu Zhang

  3. Shanxi Key Laboratory of Bone and Soft Tissue Injury Repair, Department of Orthopedics, Second Hospital of Shanxi Medical University, Taiyuan, 030024, China

    Xiang-Yu Zhang

  4. Department of Physics, Department of Materials Science and Engineering, and Department of Biomedical Engineering, City University of Hong Kong, Kowloon, Hong Kong, 999077, China

    Paul-K. Chu

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  1. Mao-Zhou Chai
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  2. Mei-Wen An
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Correspondence to Mei-Wen An or Xiang-Yu Zhang.

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Chai, MZ., An, MW., Zhang, XY. et al. In vitro and in vivo antibacterial activity of graphene oxide-modified porous TiO2 coatings under 808-nm light irradiation. Rare Met. 41, 540–545 (2022). https://doi.org/10.1007/s12598-021-01754-9

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  • DOI: https://doi.org/10.1007/s12598-021-01754-9

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