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The Journal of Physiological Sciences

, Volume 66, Issue 4, pp 327–336 | Cite as

Differences in the control of basal L-type Ca2+ current by the cyclic AMP signaling cascade in frog, rat, and human cardiac myocytes

  • Rimantas Treinys
  • Andrius Bogdelis
  • Lina Rimkutė
  • Jonas Jurevičius
  • Vytenis Arvydas SkeberdisEmail author
Original Paper

Abstract

β-adrenergic receptors (β-ARs) mediate the positive inotropic effects of catecholamines by cAMP-dependent phosphorylation of the L-type Ca2+ channels (LTCCs), which provide Ca2+ for the initiation and regulation of cell contraction. The overall effect of cAMP-modulating agents on cardiac calcium current (I Ca,L) and contraction depends on the basal activity of LTCCs which, in turn, depends on the basal activities of key enzymes involved in the cAMP signaling cascade. Our current work is a comparative study demonstrating the differences in the basal activities of β-ARs, adenylyl cyclase, phosphodiesterases, phosphatases, and LTCCs in the frog and rat ventricular and human atrial myocytes. The main conclusion is that the basal I Ca,L, and consequently the contractile function of the heart, is secured from unnecessary elevation of its activity and energy consumption at the several “checking-points” of cAMP-dependent signaling cascade and the loading of these “checking-points” may vary in different species and tissues.

Keywords

Cardiac myocytes L-type Ca2+ channels Adenylyl cyclase Phosphodiesterase Phosphatase Protein kinase A 

Notes

Acknowledgments

We thank Dr. Rodolphe Fischmeister for valuable discussions, Antanas Navalinskas for skillful technical assistance, and Valeryia Mikalayeva for preparation of the cells. This work was supported by an East–West cooperation grant from INSERM, France.

Conflict of interest

The authors declare no competing or financial interests.

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Copyright information

© The Physiological Society of Japan and Springer Japan 2015

Authors and Affiliations

  • Rimantas Treinys
    • 1
  • Andrius Bogdelis
    • 1
  • Lina Rimkutė
    • 1
  • Jonas Jurevičius
    • 1
  • Vytenis Arvydas Skeberdis
    • 1
    Email author
  1. 1.Institute of CardiologyLithuanian University of Health SciencesKaunasLithuania

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