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Nano Research

, Volume 11, Issue 7, pp 3886–3898 | Cite as

Inhibition of osteosarcoma growth and metastasis using a polysaccharide derivative of Amy-g-PLLD for the delivery of AEG-1 siRNA

  • Fen Wang
  • Jiadong Pang
  • Leilei Huang
  • Ran Wang
  • Qing Jiang
  • Liming Zhang
  • Kang Sun
Research Article
  • 102 Downloads

Abstract

Osteosarcoma is the most common primary malignant neoplasm of the bone in children and adolescents and has a high risk of relapse and metastasis. Of the various methods to treat osteosarcoma, the use of genetic approaches to inhibit the rapid growth of osteosarcoma while limiting tumor metastasis has presented a challenge in its implementation. Here, we successfully synthesized a polysaccharide derivative (Amy-g-PLLD) for delivery of astrocyte elevated gene-1 (AEG-1) small-interfering RNA (siRNA) (siAEG-1), and used it for the first time to suppress osteosarcoma tumors in vitro and in vivo. Amy-g-PLLD/ siAEG-1 complexes were delivered into 143B human osteosarcoma cells with low resultant cytotoxicity. Osteosarcoma tumor proliferation and invasion were inhibited in vitro. Intratumoral injection of Amy-g-PLLD/siAEG-1 complexes markedly inhibited tumor growth and lung metastasis in 143B tumor-bearing mice. This biocompatible and effective approach employing a natural material-siRNA complex should pave the way for more genetic research in treating osteosarcoma.

Keywords

osteosarcoma amylose gene delivery astrocyte elevated gene-1 (AEG-1) small-interfering RNA (siRNA) 

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Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (Nos. 81402221 and 51273216), the Research Fund for the Doctoral Program of Higher Education of China (No. 20130171120077), the Science and Technology Program of Guangzhou, China (No. 201707010108), the Guangdong Innovative Research Team Program (No. 2009010057), and the Science and Technology Planning Project of Guangzhou (No. 201610010006), the Science and Technology Planning project of Guangdong Province (No. 20153900042020319) and Natural Science Foundation of Guangdong Province (No. 2016A030313819).

Supplementary material

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Inhibition of osteosarcoma growth and metastasis using a polysaccharide derivative of Amy-g-PLLD for the delivery of AEG-1 siRNA

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Copyright information

© Tsinghua University Press and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of PathologyThe First Affiliated Hospital of Sun Yat-sen UniversityGuangzhouChina
  2. 2.PCFM Lab and GDHPPC Lab, School of Materials Science and EngineeringSun Yat-sen UniversityGuangzhouChina
  3. 3.School of EngineeringSun Yat-sen UniversityGuangzhouChina
  4. 4.School Guangdong Provincial Key Laboratory of Sensor Technology and Biomedical InstrumentsSun Yat-sen UniversityGuangzhouChina

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