Clinical Application of Long Non-Coding RNA-UCA1 as a Candidate Gene in Progression of Esophageal Cancer

  • Hamid Aalijahan
  • Saeid GhorbianEmail author
Original Article


Esophageal cancer (EC) is known as one of the most prevalent gastrointestinal cancers, and results in the seventh highest number of cancer-relevant deaths. Long non-coding RNAs (lncRNAs) have substantial roles in several biological processes. LncRNA human urothelial carcinoma associated 1 (UCA1) is announced to be enhanced in multiple types of human cancers. This survey was carried out to identify the potential role of the lncRNA-UCA1 in the progression of EC. A case-control investigation was performed on 140 FFPE tissues of EC patients consisting of 70 cancerous tissues and 70 marginal tissues samples. To determine the lncRNA-UCA1 gene expression changes, quantitative reverse-transcription polymerase chain reaction (qRT-PCR) method was utilized. In addition, the associations between the lncRNA-UCA1 gene expression and clinicopathological parameters were assessed. Our findings revealed that the lncRNA-UCA1 was notably up-regulated in EC tissues compared to adjacent normal tissues (P < 0.05). LncRNA-UCA1 expression was substantially correlated to alcohol drinking (P = 0.008) and socioeconomic status (P = 0.001), while shared no correlation with age, hot drinking status and stage (P > 0.05). Our data indicated that the lncRNA-UCA1 play an important role in the progression of EC and may be considered as a candidate gene in the pathogenesis of EC patients.


LncRNA-UCA1 Candidate gene Esophageal Cancer LncRNAs 



This article was extracted from a M.Sc. thesis (IR.961003) at Ahar Branch Islamic Azad University. We would like to appreciate all the patients and staffs of the Tabriz International Hospital.

Compliance with Ethical Standards

Conflict of Interest

Authors declare no conflict of interest.


  1. 1.
    Zhang Y (2013 14) Epidemiology of esophageal cancer. World J Gastroenterol: WJG 19(34):5598CrossRefGoogle Scholar
  2. 2.
    Torre LA, Bray F, Siegel RL, Ferlay J, Lortet-Tieulent J, Jemal A (2015) Global cancer statistics, 2012. CA Cancer J Clin 65(2):87–108CrossRefGoogle Scholar
  3. 3.
    Ghorbian S, Ardekani AM (2012) Non-invasive detection of esophageal Cancer using genetic changes in circulating cell-free DNA. Avicenna J Med Biotechnol 4(1):3–13Google Scholar
  4. 4.
    Song Q, Liu H, Wang J, Jia Y, Liu Y, Wang N, Tan B, Guan S, An D, Cheng Y (2014) Dinner-to-bed time and post-dinner walk: new potential independent factors in esophageal cancer development. J Cancer Res Clin Oncol 140(5):817–821CrossRefGoogle Scholar
  5. 5.
    Tang WR, Fang JY, Wu KS, Shi XJ, Luo JY, Lin K (2014) Epidemiological characteristics and prediction of esophageal cancer mortality in China from 1991 to 2012. Asian Pac J Cancer Prev 15(16):6929–6934CrossRefGoogle Scholar
  6. 6.
    Park S, Paeng JC, Kang CH, Cheon GJ, Kang KW, Chung JK, Lee DS (2018) Dual-time point 18 F-FDG PET/CT for the staging of oesophageal cancer: the best diagnostic performance by retention index for N-staging in non-calcified lymph nodes. Eur J Nucl Med Mol Imaging:1–2Google Scholar
  7. 7.
    Lanzafame M, Bianco G, Terracciano L, Ng C, Piscuoglio S (2018) The role of long non-coding RNAs in hepatocarcinogenesis. Int J Mol Sci 19(3):682CrossRefGoogle Scholar
  8. 8.
    Aalijahan H, Ghorbian S (2018) Long non-coding RNAs and cervical cancer. Exp Mol PatholGoogle Scholar
  9. 9.
    Iyer MK, Niknafs YS, Malik R, Singhal U, Sahu A, Hosono Y, Barrette TR, Prensner JR, Evans JR, Zhao S, Poliakov A (2015) The landscape of long noncoding RNAs in the human transcriptome. Nat Genet 47(3):199–208CrossRefGoogle Scholar
  10. 10.
    Cabili MN, Trapnell C, Goff L, Koziol M, Tazon-Vega B, Regev A, Rinn JL (2011) Integrative annotation of human large intergenic noncoding RNAs reveals global properties and specific subclasses. Genes Dev 2Google Scholar
  11. 11.
    Martianov I, Ramadass A, Barros AS, Chow N, Akoulitchev A (2007) Repression of the human dihydrofolate reductase gene by a non-coding interfering transcript. Nature 445(7128):666–670CrossRefGoogle Scholar
  12. 12.
    Bayoumi AS, Sayed A, Broskova Z, Teoh JP, Wilson J, Su H, Tang YL, Kim IM (2016) Crosstalk between long noncoding RNAs and microRNAs in health and disease. Int J Mol Sci 17(3):356CrossRefGoogle Scholar
  13. 13.
    Wang KC, Yang YW, Liu B, Sanyal A, Corces-Zimmerman R, Chen Y, Lajoie BR, Protacio A, Flynn RA, Gupta RA, Wysocka J (2011) A long noncoding RNA maintains active chromatin to coordinate homeotic gene expression. Nature. 472(7341):120–124CrossRefGoogle Scholar
  14. 14.
    Ye H, Lin J, Yao X, Li Y, Lin X, Lu H (2018) Overexpression of long non-coding RNA NNT-AS1 correlates with tumor progression and poor prognosis in osteosarcoma. Cell Physiol Biochem 45(5):1904–1914CrossRefGoogle Scholar
  15. 15.
    Xue M, Chen W, Li X (2016) Urothelial cancer associated 1: a long noncoding RNA with a crucial role in cancer. J Cancer Res Clin Oncol 142(7):1407–1419CrossRefGoogle Scholar
  16. 16.
    Liu FT, Dong Q, Gao H, Zhu ZM (2017) The prognostic significance of UCA1 for predicting clinical outcome in patients with digestive system malignancies. Oncotarget 8(25):40620Google Scholar
  17. 17.
    Huang J, Zhou N, Watabe K, Lu Z, Wu F, Xu M, Mo YY (2015) Long non-coding RNA UCA1 promotes breast tumor growth by suppression of p27 (Kip1). Cell Death Dis 5(1):e1008CrossRefGoogle Scholar
  18. 18.
    Yang YT, Wang YF, Lai JY, Shen SY, Wang F, Kong J, Zhang W, Yang HY (2016) Long non-coding RNA UCA 1 contributes to the progression of oral squamous cell carcinoma by regulating the WNT/β-catenin signaling pathway. Cancer Sci 107(11):1581–1589CrossRefGoogle Scholar
  19. 19.
    Bian Z, Jin L, Zhang J, Yin Y, Quan C, Hu Y, Feng Y, Liu H, Fei B, Mao Y, Zhou L (2016) LncRNA—UCA1 enhances cell proliferation and 5-fluorouracil resistance in colorectal cancer by inhibiting miR-204-5p. Sci Rep 6:23892CrossRefGoogle Scholar
  20. 20.
    Razavi M, Ghorbian S (2019) Original article - up-regulation of long non-coding RNA-PCAT-1 promotes invasion and metastasis in esophageal squamous cell carcinoma. EXCLI 18:422–428Google Scholar
  21. 21.
    Sadeghpour S, Ghorbian S (2019) Evaluation of the potential clinical prognostic value of lncRNA-BANCR gene in esophageal squamous cell carcinoma. Mol Biol Rep 46:991–995CrossRefGoogle Scholar
  22. 22.
    Qian Y, Liu D, Cao S, Tao Y, Wei D, Li W, Li G, Pan X, Lei D (2017) Upregulation of the long noncoding RNA UCA1 affects the proliferation, invasion, and survival of hypopharyngeal carcinoma. Mol Cancer 16(1):68CrossRefGoogle Scholar
  23. 23.
    Zhao W, Sun C, Cui Z (2017) A long noncoding RNA UCA1 promotes proliferation and predicts poor prognosis in glioma. Clin Transl Oncol 19(6):735–741CrossRefGoogle Scholar
  24. 24.
    Chen P, Wan D, Zheng D, Zheng Q, Wu F, Zhi Q (2016) Long non-coding RNA UCA1 promotes the tumorigenesis in pancreatic cancer. Biomed Pharmacother 83:1220–1226CrossRefGoogle Scholar
  25. 25.
    Wang HM, Lu JH, Chen WY, Gu AQ (2015) Upregulated lncRNA-UCA1 contributes to progression of lung cancer and is closely related to clinical diagnosis as a predictive biomarker in plasma. Int J Clin Exp Med 8(7):11824Google Scholar
  26. 26.
    Fang Z, Wu L, Wang L, Yang Y, Meng Y, Yang H (2014) Increased expression of the long non-coding RNA UCA1 in tongue squamous cell carcinomas: a possible correlation with cancer metastasis. Oral Surg Oral Med Oral Pathol Oral Radiol 117(1):89–95CrossRefGoogle Scholar
  27. 27.
    Huang J, Zhou N, Watabe K, Lu Z, Wu F, Xu M, Mo YY (2015) Long non-coding RNA UCA1 promotes breast tumor growth by suppression of p27 (Kip1). Cell Death Dis 5(1):e1008CrossRefGoogle Scholar
  28. 28.
    Han Y, Yang YN, Yuan HH, Zhang TT, Sui H, Wei XL, Liu L, Huang P, Zhang WJ, Bai YX (2014) UCA1, a long non-coding RNA up-regulated in colorectal cancer influences cell proliferation, apoptosis and cell cycle distribution. Pathology. 46(5):396–401CrossRefGoogle Scholar
  29. 29.
    Zheng Q, Wu F, Dai WY, Zheng DC, Zheng C, Ye H, Zhou B, Chen JJ, Chen P (2015) Aberrant expression of UCA1 in gastric cancer and its clinical significance. Clin Transl Oncol 17(8):640–646CrossRefGoogle Scholar
  30. 30.
    Wang F, Ying HQ, He BS, Pan YQ, Deng QW, Sun HL, Chen J, Liu X, Wang SK (2015) Upregulated lncRNA-UCA1 contributes to progression of hepatocellular carcinoma through inhibition of miR-216b and activation of FGFR1/ERK signaling pathway. Oncotarget 6(10):7899–7917Google Scholar
  31. 31.
    Tian Y, Zhang X, Hao Y, Fang Z, He Y (2014) Potential roles of abnormally expressed long noncoding RNA UCA1 and Malat-1 in metastasis of melanoma. Melanoma Res 24(4):335–341CrossRefGoogle Scholar
  32. 32.
    Liu SP, Yang JX, Cao DY, Shen K (2013) Identification of differentially expressed long non-coding RNAs in human ovarian cancer cells with different metastatic potentials. Cancer Biology & Medicine 10(3):138Google Scholar
  33. 33.
    Li JY, Ma X, Zhang CB (2014) Overexpression of long non-coding RNA UCA1 predicts a poor prognosis in patients with esophageal squamous cell carcinoma. Int J Clin Exp Pathol 7(11):7938Google Scholar
  34. 34.
    Wang X, Gao Z, Liao J, Shang M, Li X, Yin L, Pu Y, Liu R (2016) lncRNA UCA1 inhibits esophageal squamous-cell carcinoma growth by regulating the Wnt signaling pathway. J Toxic Environ Health A 79(9–10):407–418CrossRefGoogle Scholar
  35. 35.
    Jiao C, Song Z, Chen J, Zhong J, Cai W, Tian S, Chen S, Yi Y, Xiao Y (2016) lncRNA-UCA1 enhances cell proliferation through functioning as a ceRNA of Sox4 in esophageal cancer. Oncol Rep 36(5):2960–2966CrossRefGoogle Scholar
  36. 36.
    Deng F, Zhou K, Cui W, Liu D, Ma Y (2015) Clinicopathological significance of wnt/β-catenin signaling pathway in esophageal squamous cell carcinoma. Int J Clin Exp Pathol 8(3):3045Google Scholar

Copyright information

© Arányi Lajos Foundation 2019

Authors and Affiliations

  1. 1.Department of Molecular Genetics, Ahar BranchIslamic Azad UniversityAharIran

Personalised recommendations