Skip to main content
SpringerLink
Log in

Expression of heat shock protein 60 in the tissues of transported piglets

  • Original Paper
  • Published:
Cell Stress and Chaperones Aims and scope

Abstract

In this study, we sought to determine the distribution and expression of heat shock protein 60 (Hsp60) in the tissues of transported piglets. A total of 24 Chinese Erhualian piglets with an average body weight of 20 ± 1 kg were assessed under both 2-h transported and normal housing conditions. Results of enzymatic analysis showed that the serum creatine kinase and aspartate aminotransferase concentrations were significantly increased in the 2-h transported piglets. Acute cellular lesions characterized by granular and vacuolar degeneration of the parenchyma cells in the tested heart, liver, and kidney were also confirmed by histopathological test after 2 h transportation. These results indicate that transport stress induces tissue damage to heart, liver, and kidney. Hsp60-positive immunostaining was consistently detected in the cytoplasm of myocardial cells, hepatocytes, renal tubular epithelial cells, and epithelial cells of fundic gland. However, results of enzyme-linked immunosorbent assay indicated that Hsp60 expression was only significantly elevated in the stomach, with lower expression in the heart and a non-significant trend of increased liver and kidney expression of Hsp60. These results indicate that different tissues had different sensitivities to transport stress, possibly resulting in varying levels of cytoprotection by Hsp60 in the different tissues. The expression of Hsp60 following 2 h transportation coincided with deterioration of cardiac cytoprotection in the heart and protection in the stomach. However, the direct role of Hsp60 in cytoprotection of heart and stomach tissues needs further investigation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  • Bao ED, Sultan KR, Nowak B, Hartung J (2002) Expression and localization of HSPs in the liver of transported pigs. Agric Sci China 1:690–695

    Google Scholar 

  • Briones P, Vilaseca MA, Ribes A, Vernet A, Lluch M, Cusi V, Huckriede A, Agsteribbe E (1997) A new case of multiple mitochondrial enzyme deficiencies with decreased amount of heat shock protein 60. J Inherit Metab Dis 20:569–577

    Article  PubMed  CAS  Google Scholar 

  • Britton CV, Hernandez A, Roberts R (1980) Plasma creatine kinase isoenzyme determinations in infants and children. Characterization in normal patients and after cardiac catheterization and surgery. Chest 77:758–760

    Article  PubMed  CAS  Google Scholar 

  • Bukau B, Horwich AL (1998) The Hsp70 and Hsp60 chaperone machines. Cell 92:351–366

    Article  PubMed  CAS  Google Scholar 

  • Cumming DVE, Heads RJ, Watson A, Latchman DS, Yellon DM (1996) Differential protection of primary rat cardiocytes by transfection of specific heat stress proteins. J Mol Cell Cardiol 28:2343–2349

    Article  PubMed  CAS  Google Scholar 

  • Fàbrega E, Manteca X, Font J, Gispert M, Carrión D, Velarde A, Ruiz-de-la-Torre JL, Diestre A (2002) Effects of halothane gene and pre-slaughter treatment on meat quality and welfare from two pig crosses. Meat Sci 62:463–472

    Article  Google Scholar 

  • Fehrenbach E, Veith R, Schmid M, Dickhurh HH, Northoff H, Neiss AM (2003) Inverse response of leukocyte heat shock proteins and DNA damage to exercise and heat. Free Radic Res 37:975–982

    Article  PubMed  CAS  Google Scholar 

  • Fink AL (1999) Chaperone-mediated protein folding. Physiol Rev 79:425–449

    PubMed  CAS  Google Scholar 

  • Fu XB, Sun PM, Li YB, Wang ZL, Bao ED (2004) Tissue damages of heat-stressed broilers and expression of HSP90. J Nanjing Agric University 27:85–88

    CAS  Google Scholar 

  • Fujimori S, Otaka M, Itoh H, Kuwabara T, Zeniya A, Otani S, Tashima Y, Masamune O (1994) Induction of a 60-kDa heat shock protein in rat gastric mucosa by restraint and water-immersion stress. J Gastroenterol 29:544–546

    Article  PubMed  CAS  Google Scholar 

  • Gosálvez LF, Averós X, Valdelvira JJ, Herranz A (2006) Influence of season, distance and mixed loads on the physical and carcass integrity of pigs transported to slaughter. Meat Sci 73:553–558

    Article  Google Scholar 

  • Guo JS, Chau JFL, Cho CH, Koo MWL (2005) Partial sleep deprivation compromises gastric mucosal integrity in rats. Life Sci 77:220–229

    Article  PubMed  CAS  Google Scholar 

  • Habich C, Burkart V (2007) Heat shock protein 60: regulatory role on innate immune cells. Cell Mol Life Sci 64:742–751

    Article  PubMed  CAS  Google Scholar 

  • Hundt W, O’Connell-Rodwell CE, Bednarski MD, Steinbach S, Guccione S (2007) In vitro effect of focused ultrasound or thermal stress on HSP70 expression and cell viability in three tumor cell lines. Acad Radiol 14:859–870

    Article  PubMed  Google Scholar 

  • Iwaki K, Chi SH, Dillmann WH, Mestril R (1993) Induction of HSP70 in cultured rat neonatal cardiomyocytes by hypoxia and metabolic stress. Circulation 87:2023–2032

    PubMed  CAS  Google Scholar 

  • Jethmalani SM, Henle KJ (1997) Intracellular distribution of stress glycoproteins in a heat-resistant cell model expressing human HSP70. Biochem Biophys Res Commun 237:382–387

    Article  PubMed  CAS  Google Scholar 

  • Johnson RD, Michael L, Conner D (1995) Extreme serum elevations of aspartate aminotransferase. Am J Gastroenterol 90:1244–1245

    PubMed  CAS  Google Scholar 

  • Kim KB, Kim MH, Lee DJ (2004) The effect of exercise in cool, control and hot environments on cardioprotective HSP70 induction. J Physiol Anthropol Appl Hum Sci 23:225–230

    Article  Google Scholar 

  • Knežević M, Aleksić-Kovačević S, Aleksić Z (2007) Cell proliferation in pathogenesis of esophagogastric lesions in pigs. Int Rev Cyt 260:1–34

    Article  CAS  Google Scholar 

  • Li LA, Xia D, Bao ED et al (2007) Erhualian and Pietrain pigs exhibit distinct behavioural, endocrine and biochemical responses during transport. Livest Sci 113:169–177

    Article  Google Scholar 

  • Li YB, Bao ED, Wang ZL, Zhao RQ (2006) Detection of HSP mRNA transcription in transport stressed pigs by fluorescence quantitative RT-PCR. Agric Sci China 39:187–192

    CAS  Google Scholar 

  • Li YB, Sun PM, Wang ZL, Bao ED (2005) Relationship between pathological lesion and distribution of HSP27 in transport stressed pigs. J Nanjing Agric University 28:83–87

    Google Scholar 

  • Mallard K, Jones DB, Richmond J, McGill M, Foulis AK (1996) Expression of the human heat shock protein 60 in thyroid, pancreatic, hepatic and adrenal autoimmunity. J Autoimmun 9:89–96

    Article  PubMed  CAS  Google Scholar 

  • Mota-Rojas D, Becerril M, Lemus C, Sánchez P, González M, Olmos SA, Ramírez R, Alonso-Spilsbury M (2006) Effects of mid-summer transport duration on pre- and post-slaughter performance and pork quality in Mexico. Meat Sci 73:404–412

    Article  Google Scholar 

  • Njemini R, Bautmans I, Lambert M, Demanet C, Mets T (2007) Heat shock proteins and chemokine/cytokine secretion profile in ageing and inflammation. Mech Ageing Dev 128:450–454

    Article  PubMed  CAS  Google Scholar 

  • Oksala NKJ, Laaksonen DE, Lappalainen J, Khanna S, Nakao C, Hänninen O, Sen CK, Atalay M (2006) Heat shock protein 60 response to exercise in diabetes. Effects of α-lipoic acid supplementation. J Diabetes Complications 20:257–261

    Article  PubMed  Google Scholar 

  • Pérez MP, Palacia J, Santolaria MP, Aceña MC, Chacón G, Chacón M, Calvo JH, Zaragoza P, Beltran JA, García-Belenguer S (2002) Effect of transport time on welfare and meat quality in pigs. Meat Sci 61:425–433

    Article  Google Scholar 

  • Piñeiro M, Piñeiro C, Carpintero R, Morales J, Campbell FM, Eckersall PD, Toussaint MJM, Lampreave F (2007) Characterisation of the pig acute phase protein response to road transport. Vet J 173:669–674

    Article  PubMed  CAS  Google Scholar 

  • Salokhe S, Sarkar A, Kulkarni A, Mukherjee S, Pal JK (2006) Flufenoxuron, an acylurea insect growth regulator, alters development of Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae) by modulating levels of chitin, soluble protein content, and HSP70 and p34cdc2 in the larval tissues. Pestic Biochem Physiol 85:84–90

    Article  CAS  Google Scholar 

  • Schmitt JP, Schunkert H, Birnbaum DE, Aebert H (2002) Kinetics of heat shock protein 70 synthesis in the human heart after cold cardioplegic arrest. Eur J Cardio-thorac Surg 22:415–420

    Article  CAS  Google Scholar 

  • Snoeckx LHEH, Cornelussen RN, Van Nieuwenhoven FA, Reneman RS, Van Der Vusse GJ (2001) Heat shock proteins and cardiovascular pathophysiology. Physiol Rev 81:1461–1497

    PubMed  CAS  Google Scholar 

  • Van Laack M, Faustman C, Sebranek JC (1993) Pork quality and the expression of stress protein Hsp70 in swine. J Anim Sci 71:2958–2964

    PubMed  Google Scholar 

  • Verbeke P, Clark BFC, Rattan SIS (2001) Reduced levels of oxidized and glycoxidized proteins in human fibroblasts exposed to repeated mild heat shock during serial passaging in vitro. Free Radic Biol Med 31:1593–1602

    Article  PubMed  CAS  Google Scholar 

  • von Borell E, Schäffer D (2005) Legal requirements and assessment of stress and welfare during transportation and pre-slaughter handling of pigs. Livest Prod Sci 97:81–87

    Article  Google Scholar 

  • Wada H, Kamiike W (1990) Aspartate aminotransferase isozymes and their clinical significance. In: Ogita Z, Markert OCL (eds) Isozymes: structure, function, and use in biology and medicine, 1st ed. Wiley-Liss, New York, p 853

    Google Scholar 

  • Yu H, Bao ED, Zhao RQ, Lv QX (2007) Effect of transportation stress on heat shock protein 70 concentration and mRNA expression in heart and kidney tissues and serum enzyme activities and hormone concentrations of piglets. Am J Vet Res 68:1145–1150

    Article  PubMed  CAS  Google Scholar 

  • Zhao Q, Wang JH, Levichkin IV, Stasinopoulos S, Ryan MT, Hoogenraad NJ (2002) A mitochondrial specific stress response in mammalian cells. The EMBO Journal 21:4411–4419

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgements

This work was supported by a grant from National Natural Science Foundation of China (30430420) to Dr. Ruqian Zhao, and supported also by grants (30170682, 30571400) from the National Natural Science Foundation of China.

We would like to thank Hong Yu and Qiongxia Lv, College of Veterinary Medicine, Nanjing Agricultural University, for their technical advice.

Author information

Authors and Affiliations

  1. Key Laboratory of Animal Physiology and Biochemistry, Nanjing Agricultural University, Nanjing, 210095, China

    Lin Zhu, Endong Bao & Ruqian Zhao

  2. Institute for Animal Hygiene, Animal Welfare and Behaviour of Farm Animals, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany

    Jörg Hartung

Authors
  1. Lin Zhu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Endong Bao
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ruqian Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jörg Hartung
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Endong Bao.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhu, L., Bao, E., Zhao, R. et al. Expression of heat shock protein 60 in the tissues of transported piglets. Cell Stress and Chaperones 14, 61–69 (2009). https://doi.org/10.1007/s12192-008-0055-z

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12192-008-0055-z

Keywords

Search

Navigation