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Triple Quadrupole Mass Spectrometry with Liquid Chromatography and Dispersive Liquid-Liquid Microextraction for the Determination of Monoterpenes in Alcoholic Drinks

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Abstract

Five monoterpenes (linalool, geraniol, eugenol, carvacrol, and thymol) were directly preconcentrated from alcoholic drinks with no previous sample treatment step using a miniaturized technique based on dispersive liquid-liquid microextraction. Chloroform (300 μL) was rapidly injected into 8 mL of sample, while the addition of a disperser solvent was obviated because of the ethanol content of the alcoholic beverages. The enriched phase was evaporated, reconstituted in 50 μL water, and analyzed by reversed phase liquid chromatography with a mobile phase consisting of 25% acetonitrile (0.1% formic acid) and 75% water (0.1% formic acid) and tandem mass spectrometry with a triple quadrupole in the selected reaction monitoring mode. The matrix effect was evaluated, and quantification by the standard additions method is recommended. Limits of detection ranged from 0.003 to 1.5 ng mL−1, depending on the compound and the matrix. Enrichment factors of between 12 and 88 were obtained. The method was validated through recovery tests, finding values in the 76–128% range. The intraday precision was lower than 20% in terms of relative standard deviation. Different types of alcoholic drinks were analyzed, monoterpenes being detected at concentrations from 0.4 to 627 ng mL−1.

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Authors and Affiliations

  1. Department of Analytical Chemistry, Faculty of Chemistry, Regional Campus of International Excellence “Campus Mare Nostrum”, University of Murcia, 30100, Murcia, Spain

    Ainhoa Oller-Ruiz, Pilar Viñas, Natalia Campillo & Manuel Hernández-Córdoba

  2. Department of Food Quality and Warranty, IMIDA, c/Mayor s/n, La Alberca, 30150, Murcia, Spain

    José Fenoll

Authors
  1. Ainhoa Oller-Ruiz
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  2. Pilar Viñas
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  3. Natalia Campillo
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  4. José Fenoll
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  5. Manuel Hernández-Córdoba
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Corresponding author

Correspondence to Manuel Hernández-Córdoba.

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Funding

The authors acknowledge the financial support of the Comunidad Autónoma de la Región de Murcia (CARM, Fundación Séneca, Project 19888/GERM/15), the Spanish MINECO (Project CTQ2015-68049-R), and the European Commission (FEDER/ERDF). A. Oller-Ruiz acknowledges a fellowship from the MINECO (Plan de Garantía Juvenil).

Conflict of Interest

Ainhoa Oller-Ruiz declares that she has no conflict of interest. Pilar Viñas declares that she has no conflict of interest. Natalia Campillo declares that she has no conflict of interest. José Fenoll declares that he has no conflict of interest. Manuel Hernández-Córdoba declares that he has no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Oller-Ruiz, A., Viñas, P., Campillo, N. et al. Triple Quadrupole Mass Spectrometry with Liquid Chromatography and Dispersive Liquid-Liquid Microextraction for the Determination of Monoterpenes in Alcoholic Drinks. Food Anal. Methods 10, 3615–3622 (2017). https://doi.org/10.1007/s12161-017-0937-8

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