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QTL and Drought Effects on Leaf Physiology in Lowland Panicum virgatum

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Abstract

Switchgrass is a key component of plans to develop sustainable cellulosic ethanol production for bioenergy in the USA. We sought quantitative trait loci (QTL) for leaf structure and function, using the Albany full-sib mapping population, an F1 derived from lowland tetraploid parents. We also assessed both genotype × environment interactions (G×E) in response to drought and spatial trends within experimental plots, using the mapping population and check clones drawn from the parent cultivars. Phenotypes for leaf structure and physiological performance were determined under well-watered conditions in two consecutive years, and we applied drought to one of two replicates to test for G×E. Phenotypes for check clones varied with location in our plot and were impacted by drought, but there was limited evidence of G×E except in quantum yield (ΦPSII). Phenotypes of Albany were also influenced by plant location within our plot, and after correcting for experimental design factors and spatial effects, we detected QTL for leaf size, tissue density (LMA), and stomatal conductance (g s ). Clear evidence of G×E was detected at a QTL for intrinsic water use efficiency (iWUE) that was expressed only under drought. Loci influencing physiological traits had small additive effects, showed complex patterns of heritability, and did not co-localize with QTL for morphological traits. These insights into the genetic architecture of leaf structure and function set the stage for consideration of leaf physiological phenotypes as a component of switchgrass improvement for bioenergy purposes.

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Acknowledgments

The authors thank two anonymous reviewers and M.D. Casler for their editorial comments. We wish to thank T.S. Quedensley for assistance with clonal propagation and planting and A. Asmus for technical assistance; W. Skillern, D. Dillon, L. Taranow, Ca. Timmerman, Co. Timmerman, A. Hiers, L. Villareal, C. Lee, all students in the Freshman Research Initiative, and E. Worchel helped to collect physiological measurements. John Crutchfield and the staff of Brackenridge Field Labs were invaluable resources, particularly during construction and development of the experimental rainout shelters utilized in the study. This study was funded by a National Science Foundation Plant Genome Research Program grant to TEJ and PAF (NSF IOS-0922457). A US Department of Agriculture-Agriculture and Food Research Initiative Postdoctoral Fellowship (2011-67012-30696) supported DBL during the time that the experiments were being conducted. SHT was supported by Bowdoin College during data analysis and manuscript preparation.

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Authors and Affiliations

  1. Department of Integrative Biology, University of Texas at Austin, Austin, TX, 78712, USA

    Samuel H. Taylor, David B. Lowry, Michael J. Aspinwall, Jason E. Bonnette & Thomas E. Juenger

  2. USDA-ARS Grassland Soil and Water Research Laboratory, Temple, TX, 76502, USA

    Philip A. Fay

  3. Current Address: Departments of Environmental Studies and Biology, Keene State College, Keene, NH, 03431, USA

    Samuel H. Taylor

  4. Current Address: Department of Plant Biology, Michigan State University, East Lansing, MI, 48824, USA

    David B. Lowry

  5. Current Address: Hawkesbury Institute for the Environment, Western Sydney University, Penrith, NSW, 2751, Australia

    Michael J. Aspinwall

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  1. Samuel H. Taylor
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Correspondence to Samuel H. Taylor.

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Taylor, S.H., Lowry, D.B., Aspinwall, M.J. et al. QTL and Drought Effects on Leaf Physiology in Lowland Panicum virgatum . Bioenerg. Res. 9, 1241–1259 (2016). https://doi.org/10.1007/s12155-016-9768-5

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