Abstract
A 42-day experiment was conducted to evaluate the influence of dietary copper (Cu) concentrations on growth performance, nutrient digestibility, and serum parameters in broilers aged from 1 to 42 days. Five hundred forty 1-day-old broilers were randomly assigned into 1 of the following 6 dietary treatments: (1) control (basal diet without supplemental Cu), (2) 15 mg/kg supplemental Cu (Cu15), (3) 30 mg/kg supplemental Cu (Cu30), (4) 60 mg/kg supplemental Cu (Cu60), (5) 120 mg/kg supplemental Cu (Cu120), and (6) 240 mg/kg supplemental Cu (Cu240), Cu as copper methionine. A 4-day metabolism trial was conducted during the last week of the experiment feeding. The results showed that dietary Cu supplementation increased the average daily gain and the average daily feed intake (P < 0.01). The feed gain ratio, however, was not affected by dietary Cu (P > 0.10). Additionally, dietary Cu supplementation increased the digestibility of fat and energy (P < 0.05). The concentration of serum cholesterol, triglycerides, and high-density lipoprotein cholesterol decreased with dietary Cu supplementation (P < 0.05). The activities of serum Cu-Zn superoxide dismutase (P < 0.05), glutathione peroxidase (P < 0.05), and ceruloplasmin (P = 0.09), on the contrary, were increased by Cu addition. For immune indexes, dietary Cu supplementation increased serum IgA and IgM (P < 0.05). In addition, the activities of serum ALT increased with increasing dietary Cu supplementation (P < 0.05). In conclusion, our data suggest that Cu supplementation can increase fat digestibility and promote growth. Additionally, dietary Cu supplementation can reduce serum cholesterol and enhance antioxidant capacity in broilers.
Similar content being viewed by others
References
Jaiser SR, Winston GP (2010) Copper deficiency myelopathy. J Neurol 257(6):869–881. https://doi.org/10.1007/s00415-010-5511-x
Wang C, Wang MQ, Ye SS, Tao WJ, Du YJ (2011) Effects of copper-loaded chitosan nanoparticles on growth and immunity in broilers. Poult Sci 90(10):2223–2228. https://doi.org/10.3382/ps.2011-01511
Council NR (1994) Nutrient requirements of poultry. 9th ed edn. National Academies Press, Washington, DC
Feldpausch JA, Amachawadi RG, Tokach MD, Scott HM, Nagaraja TG, Dritz SS, Goodband RD, Woodworth JC, DeRouchey JM (2016) Effects of dietary copper, zinc, and ractopamine hydrochloride on finishing pig growth performance, carcass characteristics, and antimicrobial susceptibility of enteric bacteria. J Anim Sci 94(8):3278–3293. https://doi.org/10.2527/jas.2016-0340
Rochell SJ, Usry JL, Parr TM, Parsons CM, Dilger RN (2017) Effects of dietary copper and amino acid density on growth performance, apparent metabolizable energy, and nutrient digestibility in Eimeria acervulina-challenged broilers. Poult Sci 96(3):602–610. https://doi.org/10.3382/ps/pew276
Wu X, Liu Z, Guo J, Wan C, Zhang T, Cui H, Yang F, Gao X (2015) Influence of dietary zinc and copper on apparent mineral retention and serum biochemical indicators in young male mink (Mustela vison). Biol Trace Elem Res 165(1):59–66. https://doi.org/10.1007/s12011-014-0220-4
Ognik K, Sembratowicz I, Cholewińska E, Jankowski J, Kozłowski K, Juśkiewicz J, Zduńczyk Z (2018) The effect of administration of copper nanoparticles to chickens in their drinking water on the immune and antioxidant status of the blood. Anim Sci J 89(3):579–588. https://doi.org/10.1111/asj.12956
Chenchen XU, Wang B, Wenhua GE, Zhang M, Yue B, Shi X (2013) Effects of copper on growth performance, slaughter performance, nutrient availability and serum hormone content of Wulong Geese aged 5 to 16 weeks. Chin J Animal Nutr 25(9):1989–1997. https://doi.org/10.3969/j.issn.1006-267x.2013.09.010
Liu Z, Wu X, Zhang T, Cui H, Guo J, Guo Q, Gao X, Yang F (2016) Influence of dietary copper concentrations on growth performance, serum lipid profiles, antioxidant defenses, and fur quality in growing–furring male blue foxes (Vulpes lagopus)1. J Anim Sci 94(3):1095–1104. https://doi.org/10.2527/jas.2015-9960
Wu X, Liu Z, Zhang T, Yang Y, Yang F, Gao X (2014) Effects of dietary copper on nutrient digestibility, tissular copper deposition and fur quality of growing-furring mink (Mustela vison). Biol Trace Elem Res 158(2):166–175. https://doi.org/10.1007/s12011-014-9933-7
AOAC (2005) Official methods of analysis. 18th ed edn. Assoc. Anal. Chem., Arlington, VA
SAS (2002) Statistical Analysis System. 8.2 edn edn., Institute, Cary, NC
Shahzad MN, Javed MT, Shabir S, Irfan M, Hussain R (2012) Effects of feeding urea and copper sulphate in different combinations on live body weight, carcass weight, percent weight to body weight of different organs and histopathological tissue changes in broilers. Exp Toxicol Pathol 64(3):141–147. https://doi.org/10.1016/j.etp.2010.07.009
Gheisari AA, Sanei A, Samie A, Gheisari MM, Toghyani M (2011) Effect of diets supplemented with different levels of manganese, zinc, and copper from their organic or inorganic sources on egg production and quality characteristics in laying hens. Biol Trace Elem Res 142(3):557–571. https://doi.org/10.1007/s12011-010-8779-x
Berwanger E, Vieira SL, Angel CR, Kindlein L, Mayer AN, Ebbing MA, Lopes M (2018) Copper requirements of broiler breeder hens. Poult Sci 97(8):2785–2797. https://doi.org/10.3382/ps/pex437
Coble KF, Burnett DD, DeRouchey JM, Tokach MD, Gonzalez JM, Wu F, Dritz SS, Goodband RD, Woodworth JC, Pluske JR (2018) Effect of diet type and added copper on growth performance, carcass characteristics, energy digestibility, gut morphology, and mucosal mRNA expression of finishing pigs. J Anim Sci 96(8):3288–3301. https://doi.org/10.1093/jas/sky196
Coble KF, DeRouchey JM, Tokach MD, Dritz SS, Goodband RD, Woodworth JC, Usry JL (2017) The effects of copper source and concentration on growth performance, carcass characteristics, and pen cleanliness in finishing pigs. J Anim Sci 95(9):4052–4059. https://doi.org/10.2527/jas2017.1624
Jiao LF, Zhang QH, Wu H, Wang CC, Cao ST, Feng J, Hu CH (2018) Influences of copper/zinc-loaded montmorillonite on growth performance, mineral retention, intestinal morphology, mucosa antioxidant capacity, and cytokine contents in weaned piglets. Biol Trace Elem Res 185(2):356–363. https://doi.org/10.1007/s12011-018-1259-4
Luo XG, Dove CR (1996) Effect of dietary copper and fat on nutrient utilization, digestive enzyme activities, and tissue mineral levels in weanling pigs. J Anim Sci 74(8):1888–1896. https://doi.org/10.2527/1996.7481888x
Shahzad R, Jones MR, Viles JH, Jones CE (2016) Endocytosis of the tachykinin neuropeptide, neurokinin B, in astrocytes and its role in cellular copper uptake. J Inorg Biochem 162:319–325. https://doi.org/10.1016/j.jinorgbio.2016.02.027
Jones CE, Zandawala M, Semmens DC, Anderson S, Hanson GR, Janies DA, Elphick MR (2016) Identification of a neuropeptide precursor protein that gives rise to a “cocktail” of peptides that bind Cu(II) and generate metal-linked dimers. BBA-Gen Subjects 1860 (1, Part A):57–66. doi:https://doi.org/10.1016/j.bbagen.2015.10.008
Elizondo-Vega R, Cortes-Campos C, Barahona MJ, Carril C, Ordenes P, Salgado M, Oyarce K, Garcia-Robles ML (2016) Inhibition of hypothalamic MCT1 expression increases food intake and alters orexigenic and anorexigenic neuropeptide expression. Sci Rep 6:33606. https://doi.org/10.1038/srep33606
Dziedzic K, Szwengiel A, Gorecka D, Gujska E, Kaczkowska J, Drozdzynska A, Walkowiak J (2016) Effect of wheat dietary fiber particle size during digestion in vitro on bile acid, faecal bacteria and short-chain fatty acid content. Plant Foods Hum Nutr 71(2):151–157. https://doi.org/10.1007/s11130-016-0537-6
Xu F, Guo F, Hu XJ, Lin J (2016) Crystal structure of bile salt hydrolase from Lactobacillus salivarius. Acta Crystallogr F Struct Biol Commun 72(Pt 5):376–381. https://doi.org/10.1107/s2053230x16005707
Dijkstra M, Havinga R, Vonk RJ, Kuipers F (1996) Bile secretion of cadmium, silver, zinc and copper in the rat. Involvement of various transport systems. Life Sci 59(15):1237–1246. https://doi.org/10.1016/0024-3205(96)00447-X
Harada M, Sakisaka S, Yoshitake M, Shakadoh S, Gondoh K, Sata M, Tanikawa K (1993) Biliary copper excretion in acutely and chronically copper-loaded rats. Hepatology 17(1):111–117. https://doi.org/10.1002/hep.1840170120
Banks KM, Thompson KL, Rush JK, Applegate TJ (2004) Effects of copper source on phosphorus retention in broiler chicks and laying hens. Poult Sci 83(6):990–996. https://doi.org/10.1093/ps/83.6.990
Leeson S (2009) Copper metabolism and dietary needs. World Poultry Sci J 65(3):353–366. https://doi.org/10.1017/S0043933909000269
Cerqueira NM, Oliveira EF, Gesto DS, Santos-Martins D, Moreira C, Moorthy HN, Ramos MJ, Fernandes PA (2016) Cholesterol biosynthesis: a mechanistic overview. Biochemistry 55(39):5483–5506. https://doi.org/10.1021/acs.biochem.6b00342
Kim S, Chao PY, Allen KG (1992) Inhibition of elevated hepatic glutathione abolishes copper deficiency cholesterolemia. FASEB J 6(7):2467–2471. https://doi.org/10.1096/fasebj.6.7.1563598
Engle TE (2011) Copper and lipid metabolism in beef cattle: a review. J Anim Sci 89(2):591–596. https://doi.org/10.2527/jas.2010-3395
Helwig LR, Mulnix EJ, Regenstein J (2006) Effects of varied zinc/copper ratios on egg and plasma cholesterol level in white leghorn hens. J Food Sci 43:666–669. https://doi.org/10.1111/j.1365-2621.1978.tb02388.x
Konjufca VH, Pesti GM, Bakalli RI (1997) Modulation of cholesterol levels in broiler meat by dietary garlic and copper. Poult Sci 76(9):1264–1271. https://doi.org/10.1093/ps/76.9.1264
Maraschiello C, Sarraga C, Esteve-Garcia E, Garcia Regueiro JA (2000) Dietary iron and copper removal does not improve cholesterol and lipid oxidative stability of raw and cooked broiler meat. J Anim Sci 65(2):211–214. https://doi.org/10.1111/j.1365-2621.2000.tb15981.x
Lamb DJ, Avades TY, Ferns GA (2001) Biphasic modulation of atherosclerosis induced by graded dietary copper supplementation in the cholesterol-fed rabbit. Int J Exp Pathol 82(5):287–294
Arnal N, Castillo O, de Alaniz MJ, Marra CA (2013) Effects of copper and/or cholesterol overload on mitochondrial function in a rat model of incipient neurodegeneration. Int J Alzheimers Dis 2013:645379. https://doi.org/10.1155/2013/645379
Arnal N, Morel GR, de Alaniz MJ, Castillo O, Marra CA (2013) Role of copper and cholesterol association in the neurodegenerative process. Int J Alzheimers Dis 2013:414817–414815. https://doi.org/10.1155/2013/414817
Mehra R, Sodhi RK, Aggarwal N (2015) Memory restorative ability of clioquinol in copper-cholesterol-induced experimental dementia in mice. Pharm Biol 53(9):1250–1259. https://doi.org/10.3109/13880209.2014.974061
Chenchen XU, Wang B, Wenhua GE, Zhang M, Yue B, Zhang X, Wang X (2014) Effects of dietary different copper levels on lipid metabolism,antioxidant ability and immune function of wulong geese at the age of 5 to 16 weeks. Chin J Animal Nutr 26(4):908–917. https://doi.org/10.3969/j.issn.1006-267x.2014.04.010
Attia YA, Qota EM, Zeweil HS, Bovera F, Abd Al-Hamid AE, Sahledom MD (2012) Effect of different dietary concentrations of inorganic and organic copper on growth performance and lipid metabolism of White Pekin male ducks. Br Poult Sci 53(1):77–88. https://doi.org/10.1080/00071668.2011.650151
Pineda L, Sawosz E, Vadalasetty KP, Chwalibog A (2013) Effect of copper nanoparticles on metabolic rate and development of chicken embryos. Anim Feed Sci Technol 186(1):125–129. https://doi.org/10.1016/j.anifeedsci.2013.08.012
Narasimhaiah M, Arunachalam A, Sellappan S, Mayasula VK, Guvvala PR, Ghosh SK, Chandra V, Ghosh J, Kumar H (2018) Organic zinc and copper supplementation on antioxidant protective mechanism and their correlation with sperm functional characteristics in goats. Reprod Domest Anim 53(3):644–654. https://doi.org/10.1111/rda.13154
Michalska-Mosiej M, Socha K, Soroczynska J, Karpinska E, Lazarczyk B, Borawska MH (2016) Selenium, zinc, copper, and total antioxidant status in the serum of patients with chronic tonsillitis. Biol Trace Elem Res 173(1):30–34. https://doi.org/10.1007/s12011-016-0634-2
Kaya A, Altiner A, Ozpinar A (2006) Effect of copper deficiency on blood lipid profile and haematological parameters in broilers. J Vet Med A Physiol Pathol Clin Med 53(8):399–404. https://doi.org/10.1111/j.1439-0442.2006.00835.x
Fry RS, Ashwell MS, Lloyd KE, O'Nan AT, Flowers WL, Stewart KR, Spears JW (2012) Amount and source of dietary copper affects small intestine morphology, duodenal lipid peroxidation, hepatic oxidative stress,and mRNA expression of hepatic copper regulatory proteins in weanling pigs. J Anim Sci 90(9):3112–3119. https://doi.org/10.2527/jas.2011-4403
NRC (2005) Mineral tolerance of animals. In: 2th ed edn. National Academies Press, Washington, DC
Oğuz E, Yuksel H, Enli Y, Tufan A, Turgut G (2009) The effects of copper sulfate on liver histology and biochemical parameters of term Ross Broiler Chicks, vol 133. doi:https://doi.org/10.1007/s12011-009-8447-1
Mazija H, Prukner-Radovčić E (2002) Manual of poultry diseases Veterinarski fakultet u Zagrebu, Zagreb
Funding
The funding for this study was from Natural Science Foundation of Anhui Province of China (1708085QC74), High-level Talents Introduction Project of Anhui Institute of Science and Technology (DKYJ201701), Chuzhou Science and Technology Project (2018ZN014), and the fund of National Natural Science Foundation in Higher Education of Anhu, China (KJ2018A0535).
Author information
Authors and Affiliations
Corresponding author
Additional information
Publisher’s Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
About this article
Cite this article
Wu, X., Dai, S., Hua, J. et al. Influence of Dietary Copper Methionine Concentrations on Growth Performance, Digestibility of Nutrients, Serum Lipid Profiles, and Immune Defenses in Broilers. Biol Trace Elem Res 191, 199–206 (2019). https://doi.org/10.1007/s12011-018-1594-5
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12011-018-1594-5