Skip to main content
SpringerLink
Log in

Nitrogen Removal by Chelatococcus daeguensis TAD1 and Its Denitrification Gene Identification

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

Chelatococcus daeguensis TAD1 was demonstrated to be an aerobic denitrifier. It can utilize not only nitrate and nitrite but also ammonium at high temperature (about 50 °C). The strain had the capability to remove 122.7 and 71.7 mg L−1 NH4 +-N by 18 h at 50 and 30 °C, respectively. Triplicate heterotrophic nitrification experiments showed that 32.3 % of removed NH4 +-N was completely converted to nitrogen gas by 18 h at 50 °C. The denitrification genes involved in C. daeguensis TAD1 were identified and sequenced. It was found that the genes responsible for denitrification in TAD1 were napA, nirK, cnorB, and nosZ. Taken together, TAD1 can be an effective candidate for simultaneous nitrification and denitrificaton at high temperature.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Baek, S.-H., & Shapleigh, J. P. (2005). Applied and Environmental Microbiology, 71, 4427–4436.

    Article  CAS  Google Scholar 

  2. Saleh-Lakha, S., Shannon, K., Goyer, C., Trevors, J., Zebarth, B., & Burton, D. (2008). Applied and Environmental Microbiology, 74, 6876–6879.

    Article  CAS  Google Scholar 

  3. Bell, L. C., Richardson, D. J., & Ferguson, S. J. (1990). FEBS Letters, 265, 85–87.

    Article  CAS  Google Scholar 

  4. Su, J.-J., Liu, B.-Y., & Liu, C.-Y. (2001). Journal of Applied Microbiology, 90, 457–462.

    Article  CAS  Google Scholar 

  5. Braber, K., Robertson, L., & Kuenen, J. (1992). Antonie Van Leeuwenhoek, 62, 231–237.

    Article  Google Scholar 

  6. Miyahara, M., Kim, S. W., Fushinobu, S., Takaki, K., Yamada, T., Watanabe, A., Miyauchi, K., Endo, G., Wakagi, T., & Shoun, H. (2010). Applied and Environmental Microbiology, 76, 4619–4625.

    Article  CAS  Google Scholar 

  7. Lukow, T., & Diekmann, H. (1997). Biotechnology Letters, 19, 1157–1159.

    Article  CAS  Google Scholar 

  8. Kim, J., Park, K., Cho, K., Nam, S., Park, T., & Bajpai, R. (2005). Bioresource Technology, 96, 1897–1906.

    Article  CAS  Google Scholar 

  9. Yang, Y., Huang, S., Liang, W., Zhang, Y., Huang, H., & Xu, F. (2012). Journal of Hazardous Materials, 203–204, 326–332.

    Article  Google Scholar 

  10. Geets, J., De Cooman, M., Wittebolle, L., Heylen, K., Vanparys, B., De Vos, P., Verstraete, W., & Boon, N. (2007). Applied Microbiology and Biotechnology, 75, 211–221.

    Article  CAS  Google Scholar 

  11. Joo, H. S., Hirai, M., & Shoda, M. (2005). Biotechnology Letters, 27, 773–778.

    Article  CAS  Google Scholar 

  12. Zhang, J., Wu, P., Hao, B., & Yu, Z. (2011). Bioresource Technology, 102, 9866–9869.

    Article  CAS  Google Scholar 

  13. Philippot, L., Mirleau, P., Mazurier, S., Siblot, S., Hartmann, A., Lemanceau, P., & Germon, J. (2001). Biochimica et Biophysica Acta (BBA)-Gene Structure and Expression, 1517, 436–440.

    Article  CAS  Google Scholar 

  14. Khoroshilova, N., Popescu, C., Münck, E., Beinert, H., & Kiley, P. J. (1997). Proceedings of the National Academy of Sciences, 94, 6087.

    Article  CAS  Google Scholar 

  15. Hartig, E., Schiek, U., Vollack, K., & Zumft, W. (1999). Journal of Bacteriology, 181, 3658–3665.

    CAS  Google Scholar 

  16. Philippot, L. (2002). Biochimica et Biophysica Acta, 1577, 355–376.

    Article  CAS  Google Scholar 

  17. Darwin, A. J., Ziegelhoffer, E. C., Kiley, P. J., & Stewart, V. (1998). Journal of Bacteriology, 180, 4192–4198.

    CAS  Google Scholar 

  18. Stewart, V., Bledsoe, P. J., & Williams, S. B. (2003). Journal of Bacteriology, 185, 5862–5870.

    Article  CAS  Google Scholar 

  19. Zumft, W. G. (1997). Microbiology, 61, 533–616.

    CAS  Google Scholar 

  20. Coyne, M. S., Arunakumari, A., Averill, B. A., & Tiedje, J. M. (1989). Applied and Environmental Microbiology, 55, 2924–2931.

    CAS  Google Scholar 

  21. Hendriks, J., Oubrie, A., Castresana, J., Urbani, A., Gemeinhardt, S., & Saraste, M. (2000). Biochimica et Biophysica Acta, 1459, 266–273.

    Article  CAS  Google Scholar 

  22. Hendriks, J., Warne, A., & Gohlke, U. (1998). Biochemistry (Mosc), 37, 13102–13109.

    Article  CAS  Google Scholar 

  23. Holloway, P., McCormick, W., Watson, R. J., & Chan, Y. K. (1996). Journal of Bacteriology, 178, 1505–1514.

    CAS  Google Scholar 

  24. Chan, Y. K., McCormic, W. A., & Watson, R. J. (1997). Microbiology, 143, 2817–2824.

    Article  CAS  Google Scholar 

  25. Roussel-Delif, L., Tarnawski, S., Hamelin, J., Philippot, L., Aragno, M., & Fromin, N. (2005). Microbial Ecology, 49, 63–72.

    Article  CAS  Google Scholar 

  26. Braker, G., Fesefeldt, A., & Witzel, K. P. (1998). Applied and Environmental Microbiology, 64, 3769–3775.

    CAS  Google Scholar 

  27. Casciotti, K. L., & Ward, B. B. (2005). FEMS Microbiology Ecology, 52, 197–205.

    Article  CAS  Google Scholar 

  28. Braker, G., & Tiedje, J. M. (2003). Applied and Environmental Microbiology, 69, 3476–3483.

    Article  CAS  Google Scholar 

  29. Rösch, C., Mergel, A., & Bothe, H. (2002). Applied and Environmental Microbiology, 68, 3818–3829.

    Article  Google Scholar 

Download references

Acknowledgments

This research was financially supported by the National Natural Science Foundations of China (grant no. 20777019) and Guangdong Natural Science and Foundation (grant no. S2012020010887).

Author information

Authors and Affiliations

  1. School of Life Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, People’s Republic of China

    Yunlong Yang

  2. The Key Laboratory of Environmental Protection and Eco-Remediation of Guangdong Regular Higher Education Institutions, Guangzhou, 510006, People’s Republic of China

    Shaobin Huang, Yongqing Zhang & Fuqian Xu

  3. State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou, 510006, People’s Republic of China

    Shaobin Huang

Authors
  1. Yunlong Yang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Shaobin Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yongqing Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Fuqian Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Shaobin Huang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yang, Y., Huang, S., Zhang, Y. et al. Nitrogen Removal by Chelatococcus daeguensis TAD1 and Its Denitrification Gene Identification. Appl Biochem Biotechnol 172, 829–839 (2014). https://doi.org/10.1007/s12010-013-0590-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12010-013-0590-7

Keywords

Search

Navigation