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Characteristics and metabolic pathway of Alcaligenes sp. TB for simultaneous heterotrophic nitrification-aerobic denitrification

  • Environmental biotechnology
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Abstract

A novel heterotrophic nitrification-aerobic denitrification bacterium, Alcaligenes sp. TB (GenBank accession no. JQ044686), was isolated from a rotating drum biofilter for NO removal. Its characteristics and metabolic pathway for NO removal were comprehensively investigated. Experimental results showed that the nitrification and denitrification efficiency reached 99.42 and 96.44 %, respectively, after 44 h under the conditions of pH 7.2, 30 °C, and 120 rpm. The tests with the addition of Pb2+ and Na2WO4 as the reductase inhibitor revealed that nitrite was the key intermediate to produce the nitrogen gas as the final product in the simultaneous heterotrophic nitrification and denitrification by strain TB. Based on the experimental results, the metabolic pathway of strain TB has been proposed that it carries out shortcut/complete simultaneous nitrification and denitrification with nitrite as an intermediate and nitrogen gas as a final product. The two potential metabolic pathways existing in strain TB can be described as NH4 + → NH2OH → NO2 − → N2O → N2 and NH4 + → NH2OH → NO2 − → NO3 − → NO2 − → N2O → N2. This work indicates that the strain TB may be a good candidate for the denitrification of the sewage.

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Authors and Affiliations

  1. Engineering Research Center of the Ministry of Education for Bioconversion and Biopurification, Zhejiang University of Technology, Hangzhou, 310032, People’s Republic of China

    Jun Chen & Jianmeng Chen

  2. College of Environment, Zhejiang University of Technology, Hangzhou, 310032, People’s Republic of China

    Siyang Gu & Honghong Hao

Authors
  1. Jun Chen
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  2. Siyang Gu
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  3. Honghong Hao
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  4. Jianmeng Chen
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Correspondence to Jianmeng Chen.

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Funding

The study was funded by the National Key Research and Development Program of China (No. 2016YFC0203700), the National Natural Science Foundation of China (No. 21277125), and Program for Changjiang Scholars and Innovative Research Team in University.

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The authors declare that they have no competing interests.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Chen, J., Gu, S., Hao, H. et al. Characteristics and metabolic pathway of Alcaligenes sp. TB for simultaneous heterotrophic nitrification-aerobic denitrification. Appl Microbiol Biotechnol 100, 9787–9794 (2016). https://doi.org/10.1007/s00253-016-7840-x

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  • DOI: https://doi.org/10.1007/s00253-016-7840-x

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