Skip to main content

Advertisement

SpringerLink
Log in

Morpho-physiological and biochemical responses of tolerant and sensitive rapeseed cultivars to drought stress during early seedling growth stage

  • Original Article
  • Published:
Acta Physiologiae Plantarum Aims and scope Submit manuscript

Abstract

The edible oil production in China is highly dependent on the screening and development of drought-resistant rapeseed cultivars especially in water stress areas. However, for the successful production of rapeseed, germination and early seedling growth is the key steps under drought stress, which are not fully understood up till now. To better predict about the adoption of rapeseed cultivars to drought stress, the current study explored the presumed roles of enzymatic, non-enzymatic antioxidants and osmolytes in improving drought tolerance in rapeseed. The comparative analysis in terms of germination and early sprouting growth of six rapeseed cultivars was assessed under normal (0.00 MPa) and osmotic stresses (− 0.1, − 0.2, − 0.3, − 0.4, and − 0.5 MPa simulated by polyethylene glycol 6000). Subsequently, based on the process of germination and early sprouting growth, Shenguang 127 (SG 127) and Zhongyou 36 (ZY 36) were chosen as drought-tolerant and -sensitive rapeseed varieties, correspondingly and further evaluated under − 0.3 MPa osmotic stresses. The results indicated that drought stress rigorously hindered germination and early seedling growth of rapeseed cultivars. On the other hand, SG 127 exhibited less reduction in seedling growth paralleled with ZY 36. SG 127 revealed lower levels of hydrogen peroxide (H2O2), lipids peroxidation (MDA), electrolyte leakage (EL %) and less reduction in chlorophyll (Chl) content than ZY 36. The drought tolerance of SG 127 may be correlated with enhanced activities of enzymatic (superoxide peroxidase, catalase, and dismutase), non-enzymatic antioxidants (ascorbic acid, glutathione), more accumulation of proline; total soluble sugar and total soluble protein. Furthermore, our study highlighted the significance of enzymatic and non-enzymatic antioxidants and osmolytes in the establishment of rapeseed seedlings under drought stress. Nevertheless, the better knowledge is crucial to be further investigated on genomic and molecular basis to deeply insight the detail mechanisms of drought tolerance in rapeseed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  • Aebi H (1984) Catalase in vitro. Methods Enzym 105:121–126

    Article  CAS  Google Scholar 

  • Agarie S, Hanaoka N, Kubota F, Agata, Kaufman BP (1995) Measurement of cell membrane stability evaluated by electrolyte leakage as a drought and heat tolerance test in rice (Oryza sativa L.). J Facs Agric 40(1/2):233–240

    Google Scholar 

  • Apel KH, Hirt H (2004) Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annu Rev Plant Bio 55:373–399

    Article  CAS  Google Scholar 

  • Arjenaki FG, Dehaghi MA, Jabbari R (2011) Effects of priming on seed germination of Marigold (Calendula officinalis). Adv Environ Biol 5:276–280

    Google Scholar 

  • Ashraf M, Ahmad A, McNeilly T (2001) Growth and photosynthetic characteristics in pearl millet under water stress and different potassium supply. Photosynthetica 39(3):389–394

    Article  CAS  Google Scholar 

  • Baki AA, Anderson JD (1973) Vigour determination in soybean seed by multiple criteria. Crop Sci 13:630–632

    Article  Google Scholar 

  • Bartels D, Sunkar R (2005) Drought and salt tolerance in plants. Crit Rev Plant Sci 24:23–58

    Article  CAS  Google Scholar 

  • Beauchamp C, Fridovich I (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem 44:276–287

    Article  CAS  Google Scholar 

  • Blokhina O, Virolainen E, Fagerstedt KV (2003) Anti-oxidative damage and oxygen deprivation stress. Ann Bot 91:179–194

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Bor M, Ozdemir F, Turkan I (2003) The effect of salt stress on lipid peroxidation and antioxidants in leaves of sugar beet Beta vulgaris L. and wild beet Beta maritima L. Plant Sci 164:77–84

    Article  CAS  Google Scholar 

  • Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Article  CAS  Google Scholar 

  • Centritto M (2005) Photosynthetic limitations and carbon partitioning in cherry in response to water deficit and elevated [CO2]. Agric Ecosyst Environ 106(2–3):233–242

    Article  CAS  Google Scholar 

  • Chaturvedi GS, Singh A, Bahadur R (2012) Screening techniques for evaluating crop germplasm for drought tolerance. Plant Arch 12(1):11–18

    Google Scholar 

  • Cochard H, Coll LL, Roux X, Ameglio T (2002) Unraveling the effects of plant hydraulics on stomatal closure during water stress in walnut. Plant Physiol 128(1):282–290

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Couee I (2006) Involvement of soluble sugars in reactive oxygen species balance and responses to oxidative stress in plants. J Exp Bot 57:449–459

    Article  CAS  PubMed  Google Scholar 

  • Cuypers A, Vangronseveld J, Clijsters H (2000) Biphasic effect of copper on the ascorbate–glutathione pathway in primary leaves of Phaseolus vulgaris seedlings during the early stages of metal assimilation. Physiol Plant 110:512–517

    Article  CAS  Google Scholar 

  • Dai A, Trenberth KE, Qian TT (2004) A global data set of palmer drought severity index for 1870–2002: relationship with soil moisture and effects of surface warming. J Hydrometeorol 5:1117–1130. https://doi.org/10.1175/JHM-386.1

    Article  Google Scholar 

  • Demiral T, Turkan I (2005) Comparative lipid peroxidation, antioxidant systems and proline content in roots of two rice cultivars differing in salt tolerance. Environ Exp Bot 53:247–257

    Article  CAS  Google Scholar 

  • Dionisio-Sese ML, Tobita S (1998) Antioxidant responses of rice seedlings to salinity stress. Plant Sci 135:1–9

    Article  CAS  Google Scholar 

  • Fahad S, Chen Y, Saud S, Wang K, Xiong D, Chen C, Wu C, Shah F, Nie L, Huang J (2013) Ultraviolet radiation effect on photosynthetic pigments, biochemical attributes, antioxidant enzyme activity and hormonal contents of wheat. J Food Agri Environ 11(3&4):1635–1641

    CAS  Google Scholar 

  • Fahad S, Hussain S, Bano A, Saud S, Hassan S, Shan D, Khan FA, Khan F, Chen Y, Wu C, Tabassum MA, Chun MX, Afzal M, Jan A, Jan MT, Huang J (2014a) Potential role of phytohormones and plant growth-promoting rhizobacteria in abiotic stresses: consequences for changing environment. Environ Sci Pollut Res https://doi.org/10.1007/s11356-014-3754-2

    Article  Google Scholar 

  • Fahad S, Hussain S, Matloob A, Khan FA, Khaliq A, Saud S, Hassan S, Shan D, Khan F, Ullah N, Faiq M, Khan MR, Tareen AK, Khan A, Ullah A, Ullah N, Huang J (2014b) Phytohormones and plant responses to salinity stress: a review. Plant Growth Regul DOI. https://doi.org/10.1007/s10725-014-0013-y

    Article  Google Scholar 

  • Fahad S, Hussain S, Saud S, Tanveer M, Bajwa AA, Hassan S, Shah AN, Ullah A, Wu C, Khan FA, Shah F, Ullah S, Chen Y, Huang J (2015a) A biochar application protects rice pollen from high-temperature stress. Plant Physiol Biochem 96:281–287

    Article  CAS  PubMed  Google Scholar 

  • Fahad S, Nie L, Chen Y, Wu C, Xiong D, Saud S, Hongyan L, Cui K, Huang J (2015b) Crop plant hormones and environmental stress. Sustain Agric Rev 15:371–400

    Article  Google Scholar 

  • Fahad S, Hussain S, Saud S, Hassan S, Chauhan BS, Khan F (2016a) Responses of rapid viscoanalyzer profile and other rice grain qualities to exogenously applied plant growth regulators under high day and high night temperatures. PLoS One 11(7):e0159590. 1371/journal.pone.0159590

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Fahad S, Hussain S, Saud S, Khan F, Hassan S Jr, Nasim A, Arif W, Wang M, Huang F J (2016b) Exogenously applied plant growth regulators affect heat-stressed rice pollens. J Agron Crop Sci 202:139–150

    Article  CAS  Google Scholar 

  • Fahad S, Hussain S, Saud S, Hassan S, Ihsan Z, Shah AN, Wu C, Yousaf M, Nasim W, Alharby H, Alghabari F, Huang J (2016c) Exogenously applied plant growth regulators enhance the morphophysiological growth and yield of rice under high temperature. Front Plant Sci 7:1250. https://doi.org/10.3389/fpls.2016.01250

    Article  PubMed  PubMed Central  Google Scholar 

  • Fahad S, Hussain S, Saud S, Hassan S, Tanveer M, Ihsan MZ, Shah AN, Ullah A, Nasrullah KF, Ullah S, Alharby H, Wu C, Huang J (2016d) A combined application of biochar and phosphorus alleviates heat-induced adversities on physiological, agronomical and quality attributes of rice. Plant Physiol Biochem 103:191–198

    Article  CAS  PubMed  Google Scholar 

  • Fahad S, Bajwa AA, Nazir U, Anjum SA, Farooq A, Zohaib A, Sadia S, Nasim W, Adkins S, Saud S, Ihsan MZ, Alharby H, Wu C, Wang D, Huang J (2017) Crop production under drought and heat Stress: plant responses and management options. Front Plant Sci 8:1147. https://doi.org/10.3389/fpls.2017.01147

    Article  PubMed  PubMed Central  Google Scholar 

  • Fahad S, Muhammad ZI, Abdul K, Ihsanullah D, Saud S, Saleh A, Wajid N, Muhammad A, Imtiaz AK, Chao W, Depeng W, Jianliang H (2018) Consequences of high temperature under changing climate optima for rice pollen characteristics-concepts and perspectives. Arch Agron Soil Sci. https://doi.org/10.1080/03650340.2018.1443213

    Article  Google Scholar 

  • Food and Agricultural Organization (FAO) (2011) Land and plant nutrition management service. http://www.fao.org/wsfs/forum2050/

  • Griffith OW (1980) Determination of glutathione and glutathione disulfide using glutathione reductase and 2-vinylpyridine. Anal Biochem 106(1):207

    Article  CAS  PubMed  Google Scholar 

  • Guo TR, Zhang GP, Zhang YH (2007) Physiological changes in barley plants under combined toxicity of aluminum, copper and cadmium. Colloids Surf B Biointerfaces 57:182–188

    Article  CAS  PubMed  Google Scholar 

  • Gupta N, Thind SK, Bains NS (2014) Glycine betaine application modifies biochemical attributes of osmotic adjustment in drought stressed wheat. Plant Growth Regul 72(3):221–228

    Article  CAS  Google Scholar 

  • Hafiz MH, Wajid F, Farhat A, Fahad S, Shafqat S, Wajid N, Hafiz FB (2016) Maize plant nitrogen uptake dynamics at limited irrigation water and nitrogen. Environ Sci Pollut Res. https://doi.org/10.1007/s11356-016-8031-0

    Article  Google Scholar 

  • Hafiz MH, Farhat A, Shafqat S, Fahad S, Artemi C, Wajid F, Chaves CB, Wajid N, Muhammad M, Hafiz FB (2018) Offsetting land degradation through nitrogen and water management during maize cultivation under arid conditions. Land Degrad Dev 29:1366–1375

    Article  Google Scholar 

  • Harris D, Pathan AK, Gothkar P, Joshi A, Chivasa W, Nyamudeza P (2001) On farm seed priming: using participatory methods to revive and refine a key technology. Agric Syst 69:151–164

    Article  Google Scholar 

  • Hasanuzzaman M, Nahar K, Alam M, Roychowdhury (2013) Physiological, biochemical, and molecular mechanisms of heat stress tolerance in plants. Int J Mol Sci 14:9643–9684

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Archiv Biochem Biophy 125:189–198

    Article  CAS  Google Scholar 

  • Hsiao TC, Xu LK (2000) Sensitivity of growth of roots versus leaves to water stress: biophysical analysis and relation to water transport. J Exp Bot 51:1595–1616

    Article  CAS  PubMed  Google Scholar 

  • Jabbari H, Akbari GA, Khosh Kholgh Sima NA, Shirani Rad AH, Alahdadi I, Hamed A, Shariatpanahi ME (2013) Relationships between seedling establishment and soil moisture content for winter and spring rapeseed genotypes. Ind Crops Prod 49:177–187

    Article  Google Scholar 

  • Jagtap V, Bhargava S (1995) Variation in the antioxidant metabolism of drought tolerant and drought susceptible varieties of sorghum bicolor (L.). J Plant Physiol 145:195–197

    Article  CAS  Google Scholar 

  • Jaleel CA, Manivannan P, Lakshmanan GMA, Gomathinayagam M, Panneerselvam R (2008) Alterations in morphological parameters and photosynthetic pigment responses of Catharanthus roseus under soil water deficits. Coll Surf B: Biointer 61(2):298–303

    Article  CAS  Google Scholar 

  • Kalefetoglu Macar T, Ekmekci Y (2009) Alterations in photochemical and physiological activities of chickpea (Cicer arietinum L.) cultivars under drought stress. J Agron Crop Sci 195(5):335–346

    Article  CAS  Google Scholar 

  • Kaur K, Kaur N, Gupta AK, Singh I (2013) Exploration of the antioxidative defense system to characterize chickpea genotypes showing differential response towards water deficit conditions. Plant Growth Regul 70(1):49–60

    Article  CAS  Google Scholar 

  • Ku Zniak E, Skłodowska M (2001) Ascorbate, glutathione and related enzymes in chloroplasts of tomato leaves infected by Botrytis cinerea. Plant Sci 160:723–731

    Article  CAS  Google Scholar 

  • Lee SS, Kim JH (2000) Total sugars, a-amylase activity, and emergence after priming of normal and aged rice seeds. Korean Crop Sci 45:108–111

    Google Scholar 

  • Lefèvre I, Marchal G, Ghanem ME, Correal E, Lutts S (2010) Cadmium has contrasting effects on polyethylene glycol-sensitive and resistant cell lines in the mediterranean halophytes species Atriplex halimus L. Plant Physiol 167:365–374

    Article  CAS  Google Scholar 

  • Leng GY, Tang QH, Rayburg S (2015) Climate change impacts on meteorological, agricultural and hydrological droughts in China. Glob Planet Change 126:23–34

    Article  Google Scholar 

  • Li Z, Wu BJ, Lu GY, Chen Y, Zou CS, Zhang XK (2012) Differences in physiological responses of brassica napus genotypes under water stress during seedling stage. J Oil Crops Sci 34:033–039

    Google Scholar 

  • Lichtenthaler HK, Wellburn AR (1983) Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents. Biochem Soc Trans 11:591–592

    Article  CAS  Google Scholar 

  • Liu D, Pei ZF, Naeem MS, Ming DF, Liu DF, Khan F, Zhou WJ (2011) 5-Aminolevulinic acid activates antioxidative defence system and seedling growth in Brassica napus L. under water-deficit stress. J Agron Crop Sci 197(4):284–295

    Article  CAS  Google Scholar 

  • Liu D, Wu L, Naeem MS, Liu H, Deng X, Xu L, Zhang F, Zhou W (2013) 5-Aminolevulinic acid enhances photosynthetic gas exchange, chlorophyll fluorescence and antioxidant system in oilseed rape under drought stress. Acta Physiol Plant 35:2747–2759

    Article  CAS  Google Scholar 

  • Liu D, Hu LY, Ali B, Yang AG, Wan GL, Xu L, Zhou WJ (2016) Influence of 5-aminolevulinic acid on photosynthetically related parameters and gene expression in Brassica napus L. under drought stress. Soil Sci Plant Nutri 62(3):254–262

    Article  CAS  Google Scholar 

  • Ma Z, Fu C (2003) Interannual characteristics of the surface hydrological variables over the arid and semi-arid areas of northern China. Glob Planet Change 37:189–200. https://doi.org/10.1016/S0921-8181(02)00203-5

    Article  Google Scholar 

  • Michel BE, Kaufmann MR (1973) The osmotic potential of polyethylene glycol 6000. Plant Physiol 51:914–916

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Muhammad T, Shakeel A, Fahad S, Ghulam A, Sajjad H, Zartash F, Wajid N, Muhammad M, Muhammad HUR, Muhammad AK, Muhammad A, Carol JW, Gerrit H (2018) The impact of climate warming and crop management on phenology of sunflower-based cropping systems in Punjab, Pakistan. Agric For Meteorol 256–257:270–282

    Google Scholar 

  • Naya L, Ladrera R, Ramos J, Gonzalez EM, Arrese-Igor C, Minchin FR, Becana M (2007) The response of carbon metabolism and antioxidant defenses of alfalfa nodules to drought stress and to the subsequent recovery of plants. Plant Physiol 144:1104–1114

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Porcel R, Ruiz-Lozano JM (2004) Arbuscular mycorrhizal influence on leaf water potential, solute accumulation and oxidative stress in soybean plants subjected to drought stress. J Exp Bot 55:1743–1750

    Article  CAS  PubMed  Google Scholar 

  • Puckette MC, Weng H, Mahalingam R (2007) Physiological and biochemical responses to acute ozone-induced oxidative stress in Medicago truncatula. Plant Physiol Biochem 45:70–79

    Article  CAS  PubMed  Google Scholar 

  • Qaderi MM, Kurepin LV, Reid DM (2012) Effects of temperature and watering regime on growth gas exchange and abscisic acid content of canola (Brassica napus L.) seedlings. Environ Exp Bot 75:107–113

    Article  CAS  Google Scholar 

  • Reddy AR, Chaitanya KV, Vivekanandan M (2004) Drought-induced responses of photosynthesis and antioxidant metabolism in higher plants. J Plant Physiol 161:1189–1202

    Article  CAS  Google Scholar 

  • Sabra A, Daayf F, Renault S (2012) Differential physiological and biochemical responses of three Echinacea species to salinity stress. Scientia Hort 135:23–31

    Article  CAS  Google Scholar 

  • Sajid H, Chu Z, Zhigang B, Xiaochuang C, Lianfeng Z, Azhar H, Chunquan Z, Fahad S, Allen BJ, Junhua Z, Qianyu J (2018) Effects of 1-Methylcyclopropene on rice growth characteristics and superior and inferior spikelet development under salt stress. J Plant Growth Regul. https://doi.org/10.1007/s00344-018-9800-4

    Article  Google Scholar 

  • Sangtarash MH, Qaderi MM, Chinnappa CC, Reid DM (2009) Differential sensitivity of canola (Brassica napus L.) seedlings to ultraviolet-B radiation, water stress and abscisic acid. Environ Exp Bot 66:212–219

    Article  CAS  Google Scholar 

  • Saud S, Chen Y, Long B, Fahad S, Sadiq A (2013) The different impact on the growth of cool season turf grass under the various conditions on salinity and draught stress. Int J Agric Sci Res 3:77–84

    Google Scholar 

  • Saud S, Li X, Chen Y, Zhang L, Fahad S, Hussain S, Sadiq A, Chen Y (2014) Silicon application increases drought tolerance of Kentucky bluegrass by improving plant water relations and morphophysiological functions. Sci World J https://doi.org/10.1155/2014/368694

    Article  Google Scholar 

  • Saud S, Chen Y, Fahad S, Hussain S, Na L, Xin L, Alhussien SAAFE (2016) Silicate application increases the photosynthesis and its associated metabolic activities in Kentucky bluegrass under drought stress and post-drought recovery. Environ Sci Pollut Res. https://doi.org/10.1007/s11356-016-6957-x

    Article  Google Scholar 

  • Saud S, Fahad S, Yajun C, Ihsan MZ, Hammad HM, Nasim W, Amanullah Jr, Arif M, Alharby H (2017) Effects of nitrogen supply on water stress and recovery mechanisms in kentucky bluegrass plants. Front Plant Sci 8:983. https://doi.org/10.3389/fpls.2017.00983

    Article  PubMed  PubMed Central  Google Scholar 

  • Sheffield J, Wood EF (2008) Projected changes in drought occurrence under future global warming from multi-model, multi-scenario, IPCC AR4 simulations. Clim Dyn 31:79–105. https://doi.org/10.1007/s00382-007-0340-z

    Article  Google Scholar 

  • Szalai G, Kellos T, Galiba G, Kocsy G (2009) Glutathione as an antioxidant and regulatory molecule in plants under abiotic stress conditions. J Plant Growth Regul 28(1):66–80

    Article  CAS  Google Scholar 

  • Tian H, Ren W, Tao B, Sun G, Chappelka A, Wang X, Pan S, Yang J, Liu J, Felzer B, Melillo J, Reilly J (2016) Climate extremes and ozone pollution: a growing threat to China’s food security. Ecosyst Health Sustain 2(1):1203. https://doi.org/10.1002/ehs2.1203

    Article  Google Scholar 

  • Trenberth KE, Dai A, Schrier G, Jones PD, Barichivich J, Briffa KR, Sheffield J (2014) Global warming and changes in drought. Nat Clim Change 4:17–22. https://doi.org/10.1038/nclimate2067

    Article  Google Scholar 

  • Turkan I, Bor M, Ozdemir F, Koca H (2005) Differential responses of lipid peroxidation and antioxidants in the leaves of drought-tolerant P. acutifolius Gray and drought-sensitive P. vulgaris L. subjected to polyethylene glycol mediated water stress. Plant Sci 168:223–231

    Article  CAS  Google Scholar 

  • Verbrugeen N, Hermans C (2008) Proline accumulation in plants: a review. Amino Acids 35:753–759

    Article  CAS  Google Scholar 

  • Wang WB, Kim YH, Lee HS, Kim KY, Deng XP, Kwak SS (2009) Analysis of antioxidant enzyme activity during germination of alfalfa under salt and drought stresses. Plant Physiol Biochem 47:570–577

    Article  CAS  PubMed  Google Scholar 

  • Wetherald RT, Manabe S (2002) Simulation of hydrologic changes associated with global warming. J Geophys Res Atmos. https://doi.org/10.1029/2001jd001195. (ACL 7-1-ACL 7–15)

    Article  Google Scholar 

  • Wu W, Ma BL (2018) Assessment of canola crop lodging under elevated temperatures for adaptation to climate change. Agric For Meteorol 248:329–338. https://doi.org/10.1016/j.agrformet.2017.09.017

    Article  Google Scholar 

  • Wu W, Ma BL, Whalen JK (2018) Enhancing rapeseed tolerance to heat and drought stresses in a changing climate: perspectives for stress adaptation from root system architecture. Adv Agron 151:1–60

    Article  Google Scholar 

  • Zare M, Mehrabioladi AA, Sharafzadeh S (2006) Investigation of GA3 and kinetin effects on seed germination and seedling growth of wheat under salinity stress. J Agric Sci 12(4):855–865

    Google Scholar 

  • Zhang X (1992) The measurement and mechanism of lipid peroxidation and SOD, POD and CAT activities in biological system. Research Methodology of Crop Physiology. Agriculture Press, Beijing, pp 208–211

    Google Scholar 

  • Zhang J, Kirkham MB (1996) Antioxidant responses to drought in sunflower and sorghum seedlings. New Phytol 132:361–373

    Article  CAS  PubMed  Google Scholar 

  • Zhang X, Lu G, Long W, Zou X, Li F, Nishio T (2014) Recent progress in drought and salt tolerance studies in Brassica crops. Breeding Sci 64:60–73

    Article  CAS  Google Scholar 

  • Zhang J, Jiang F, Yang P, Li J, Yan G, Hu L (2015a) Responses of canola (Brassica napus L.) cultivars under contrasting temperature regimes during early seedling growth stage as revealed by multiple physiological criteria. Acta Phyiol Plant 37:7

    Article  CAS  Google Scholar 

  • Zhang J, Mason AS, Liu JWS, Zhang X, Luo T, Redden R, Batley J, Hu L, Yan G (2015b) Identification of putative candidate genes for water stress tolerance in canola (Brassica napus). Front Plant Sci 6:1–13

    Google Scholar 

  • Zhang M, Jin ZQ, Zhao J, Zhang G, Wu F (2015c) Physiological and biochemical responses to drought stress in cultivated and Tibetan wild barley. Plant Growth Regul 75:567–574

    Article  CAS  Google Scholar 

  • Zhou ZS, Huang SQ, Guo K, Kant Meht S, Zhang PC, Yang ZM (2007) Metabolic adaptations to mercury-induced oxidative stress in roots of Medicago sativa L. J Inorg Biochem 10:11–19

    Google Scholar 

  • Zhou ZS, Wang SJ, Yang ZM (2008) Biological detection and analysis of mercury toxicity to alfalfa (Medicago sativa) plants. Chemosphere 70:1500–1509

    Article  CAS  PubMed  Google Scholar 

  • Zhou ZS, Guo K, Abdou A, Elbaz Yang ZM (2009) Salicylic acid alleviates mercury toxicity by preventing oxidative stress in roots of Medicago sativa. Environ Exp Bot 65:27–34

    Article  CAS  Google Scholar 

Download references

Acknowledgements

The authors are grateful to National Natural Science Foundation of China (31271664) for funding this research.

Author information

Authors and Affiliations

  1. MOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China

    Mohammad Nauman Khan, Jing Zhang, Tao Luo, Jiahuan Liu, Fei Ni, Shah Fahad & Liyong Hu

  2. Department of Agriculture, The University of Swabi, Khyber Pakhtunkhwa, Pakistan

    Shah Fahad

  3. Microelement Research Centre, College of Resources and Environment, Huazhong Agricultural University, Wuhan, 430070, China

    Muhammad Rizwan

Authors
  1. Mohammad Nauman Khan
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jing Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Tao Luo
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jiahuan Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Fei Ni
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Muhammad Rizwan
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Shah Fahad
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Liyong Hu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Liyong Hu.

Additional information

Communicated by W. Zhou.

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 515 KB)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Khan, M.N., Zhang, J., Luo, T. et al. Morpho-physiological and biochemical responses of tolerant and sensitive rapeseed cultivars to drought stress during early seedling growth stage. Acta Physiol Plant 41, 25 (2019). https://doi.org/10.1007/s11738-019-2812-2

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s11738-019-2812-2

Keywords

Search

Navigation