Optimization of a Technique to Standardize the Rodent Roux-En-Y Gastric Bypass Model and Troubleshooting of Postoperative Failures
- 106 Downloads
Rodent models are required in studies on the mechanism of Roux-en-Y gastric bypass (RYGB). However, the construction of the model is hard, and there are various causes of death after surgery in rats.
RYGB models with procedures containing a series of anatomic landmark were established in rats. Optimized procedures during surgery, possible complications after surgery, and corresponding solutions were studied.
With the introduction of perioperative nursing and optimized surgery procedures, less time-consuming surgeries were performed and higher survival rates were achieved. Trouble-shooting data based on death time points are listed and discussed for various causes of failure.
This study provides practical suggestions for investigators to perform RYGB surgery on rats. The troubleshooting suggestions will help operators to efficiently identify problems in their procedures.
KeywordsRYGB Rodent model Surgical procedure Trouble shooting
This study was supported by National Key Basic Research Program of China (No. 2015CB5540007); National Natural Science Foundation of China (Nos. 81472740, 81200276, and 81700488); Natural Science Foundation of Hubei Province of China (No. 2014CFA060 and 2015CFB710); Research Fund of Public Welfare in Health Industry, Health and Family Plan Committee of China (No. 201402015); Natural Science Foundation of Huazhong University of Science and Technology (No. 5001530030); and Health and Family Planning Youth Project Foundation of Hubei Province, China (No. WJ2015Q001).
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no conflicts of interest.
A Statement of Animal Rights/Ethical Approval
The Ethics Committee for Animal Research of Tongji Medicine College approved all procedures in this study.
- 1.NCD Risk Factor Collaboration (NCD-RisC). Trends in adult body-mass index in 200 countries from 1975 to 2014: a pooled analysis of 1698 population-based measurement studies with 19·2 million participants. Lancet. 2016;387(1002 6):1377–96.Google Scholar
- 6.Bueter M, Abegg K, Seyfried F, et al. Roux-en-Y gastric bypass operation in rats. J Vis Exp. 2012;(64):e3940. https://doi.org/10.3791/3940.
- 8.Mason EE, Printen KJ, Lewis JW, et al. Gastric bypass criteria for effectiveness. Int J Obes. 1981;5(4):405–11.Google Scholar
- 17.Scopinaro N, Gianetta E, Pandolfo N, et al. Bilio-pancreatic bypass. Proposal and preliminary experimental study of a new type of operation for the functional surgical treatment of obesity. Minerva Chir. 1976;31(10):560–6.Google Scholar
- 18.Patrício BG, Morais T, Guimarães M, et al. Gut hormone release after gastric bypass depends on the length of the biliopancreatic limb. Int J Obes (Lond). 2018. https://doi.org/10.1038/s41366-018-0117-y.
- 27.Shan CX, Qiu NC, Liu ME, et al. Effects of diet on bile acid metabolism and insulin resistance in type 2 diabetic rats after Roux-en-Y gastric bypass. Obes Surg. 2018;28(10):3044–53. https://doi.org/10.1007/s11695-018-3264-2.