Abstract
A liquid paraffin overlay (LPO) was used for storage of rapidly multiplying somatic embryos of Dendrocalamus hamiltonii under slow-growth conditions. Slow growth was associated with changes in sugar metabolism. In rapidly growing embryogenic tissues, a sharp decline in starch and non-reducing sugars indicated rapid utilization of starch. In contrast, under slow-growth conditions in somatic embryos stored under LPO, a gradual decline in starch indicated its slower utilization. As a result, growth of somatic embryos under LPO was suppressed and subculturing was not required. Following retrieval from growth under LPO after 30, 90, 180, 270, and 365 d of storage, the somatic embryos showed high recovery and germination (79.78%, 77.49%, 71.22%, 67.13%, and 59.99%, respectively) and were able to proliferate following transfer to Murashige and Skoog’s (Physiol. Plant. 15:473–497, 1962) medium containing 1 mgl−1 BAP and 2% sucrose. The study provides useful information on in vitro storage of embryogenic tissue of D. hamiltonii.
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References
Adams C. A.; Rinnie R. W.; Fjerstad M. C. Starch deposition and carbohydrates activity in developing and germinating soybean seeds. Ann. Bot. 45: 577–582; 1980.
Augereau J. M.; Courtois D.; Petiard V. Long term storage of callus at low temperatures or under mineral oil layer. Plant Cell Rep. 5: 372–376; 1986.
Barclay A. M.; Carwford R. M. M. The effect of anaerobiosis on carbohydrate levels in storage tissues of wetland plants. Ann. Bot. 51: 255–259; 1983.
Bhattacharya A.; Saini U.; Sharma P.; Nagar P. K.; Ahuja P. S. Osmotin-regulated reserve accumulation and germination in genetically transformed tea somatic embryos: a step towards regulation of stress tolerance and seed recalcitrance. Seed Sci. Res. 16: 203–211; 2006.
Caplin S. M. Mineral oil overlay for conservation of plant tissue cultures. Am. J. Bot. 46: 324–329; 1959.
Cervelli R.; Seneranta T. Economic aspects of somatic embryogenesis. In: Aitken J. M.; Kozai T.; Smith M. A. (eds) Automation and environmental control in plant tissue culture. Kluwer, Dordrecht, The Netherlands, pp 29–64; 1995.
Chambers S. M.; Heuch J. H. R.; Pirrie A. Micropropagation and in vitro flowering of bamboo Dendrocalamus hamiltonii Munro. Plant Cell Tiss. Org. Cult. 27: 45–48; 1991.
Dubois M.; Gilles K. A.; Hamilton J. K.; Rebers P. A.; Smith F. Colorimetric method for determination of sugars and related substances. Anal. Chem. 28: 350–356; 1956.
Godbole S.; Sood A.; Sharma M.; Nagar P. K.; Ahuja P. S. Starch deposition and amylase accumulation during somatic embryogenesis in bamboo (Dendrocalamus hamiltonii). J. Plant Physiol. 161: 245–248; 2004.
Godbole S.; Sood A.; Thakur R.; Sharma M.; Ahuja P. S. Somatic embryogenesis and its conversion into plantlets in a multipurpose bamboo Dendrocalamus hamiltonii Nees et Arn. Ex. Munro. Curr. Sci. 83: 885–889; 2002.
Harding K. The methylation status of DNA derived from patato plants recovered from slow growth. Plant Cell Tiss. Org. Cult. 37: 31–38; 1994.
Jang J. C.; Zhou P. L.; Sheen J. Hexokinase as a sugar sensor in higher plants. Plant Cell 9: 5–19; 1997.
Kushwaha R.; Pandey S.; Chanda S.; Bhattacharya A.; Ahuja P. S. GA3 induced changes in slow growing endangered Himalayan plant Podophyllum hexandrum and hastening of vegetative growth. Plant Growth Regul. 51: 207–215; 2007.
Miller G. L. Use of dinitrosalicylic acid reagent for the determination of reducing sugar. Anal. Chem. 31: 426–428; 1959.
Moriguchi T.; Kozaki I.; Matsuta N.; Yamaki S. Plant regeneration from grape callus under a combination of low temperature and silicone treatment. Plant Cell Tiss. Org. Cult. 15: 67–71; 1988.
Murashige T.; Skoog F. A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol. Plant. 15: 473–497; 1962.
Ogita S. Callus and cell suspension culture of bamboo plant, Phyllostachys nigra. Plant Biotehnol. 22: 119–125; 2005.
Rawyler A.; Arpagaus S.; Braendle R. Impact of oxygen stress and energy availability on membrane stability of plant cells. Ann. Bot. 90: 499–507; 2002.
Sharma YML (1980) Bamboo research in Asia Pacific region. In: Lessard G, Chouinard A (eds) Proceedings of a workshop on bamboo research in Asia, Singapore, International Development Research Centre, Ottawa, Canada, pp. 99–120
Sood A.; Ahuja P. S.; Sharma M.; Sharma O. P.; Godbole S. In vitro protocols and field performance of elites of an important bamboo Dendrocalamus hamiltonii Nees et Arn. Ex. Munro. Plant Cell Tiss. Org. Cult. 71: 55–63; 2002a.
Sood A.; Palni L. M. S.; Sharma M.; Chand G.; Sharma O. P. Micropropagation of Dendrocalamus hamiltonii Munro (Magar Bamboo) using explants taken from seed-raised and field-tested plants. J. Plant Biol. 29(2): 125–132; 2002b.
Sutton B. C. S.; Polonenko D. R. Commercialization of plant somatic embryogenesis. In: Jain S. M.; Gupta P. K.; Newton R. J. (eds) Somatic embryogenesis in woody plants, forestry sciences, vol. 4. Kulwer, Dordrecht, The Netherlands, pp 263–291; 1999.
Withers L. A. In vitro conservation. Biol. J. Linn. Soc. 43: 31–42; 1991.
Acknowledgments
The authors gratefully acknowledge the Council of Scientific and Industrial Research and the Director, Dr. P S Ahuja, for facilitating the entire work. The financial assistance provided by the Department of Biotechnology, Govt. of India is gratefully acknowledged by the authors. The authors also thank Dr. R D Singh for his help in statistical analyses of the results.
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Editor: J. Finer
IHBT Publication No.: 0837
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Kaur, D., Ogra, R.K., Bhattacharya, A. et al. Changes in sugar levels during slow growth of Dendrocalamus hamiltonii somatic embryos due to liquid paraffin overlay. In Vitro Cell.Dev.Biol.-Plant 48, 120–126 (2012). https://doi.org/10.1007/s11627-011-9392-1
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DOI: https://doi.org/10.1007/s11627-011-9392-1