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Chinese Science Bulletin

, Volume 58, Issue 9, pp 1019–1027 | Cite as

Spatial variability of cyanobacterial community composition in Sanya Bay as determined by DGGE fingerprinting and multivariate analysis

  • Juan Ling
  • YanYing Zhang
  • JunDe DongEmail author
  • YouShao Wang
  • Hui Huang
  • Lei Chen
  • XiaoFang Huang
  • LiJuan Long
  • Si Zhang
Open Access
Article Oceanology

Abstract

The cyanobacterial communities in the surface and bottom waters of Sanya Bay were investigated on April 24 and 25, 2010. Flow cytometry showed that the total cyanobacterial abundance in the surface and bottom layers ranged from 0.7×104 to 2.38×104 cells mL−1 and from 1×104 to 1.8×104 cells mL−1, respectively. Cyanobacterial diversity was analyzed using a molecular fingerprinting technique called denaturing gradient gel electrophoresis (DGGE), followed by DNA sequencing. The results were then interpreted through multivariate statistical analysis. Differences in the compositions of cyanobacterial communities were observed in the surface and bottom waters at the same station, with some bands obtained from both the surface and bottom layers, whereas some bands were present only in one layer. The predominant cyanobacterial species of the excised DGGE bands were related to Synechococcus or Synechococcus-like species (56.2%). Other phylogenetic groups identified included Chroococcidiopsis (6.3%), Cyanobium (6.3%) and some unclassified cyanobacteria (31.2%). A redundancy analysis (RDA) was conducted to reveal the relationships between the cyanobacterial community composition and environmental factors. Analysis results showed that the spatial variations in the cyanobacterial community composition in surface waters was significantly related to chlorophyll a (Chla), the biochemical oxygen demand (BOD), nitrate and phosphate (P<0.05). Meanwhile, the spatial variations in the bottom waters was significantly affected by nitrate, nitrite, and phosphate (P<0.05). Environmental parameters could explain 99.3% and 58.3% of the variations in the surface and bottom layers, respectively.

Keywords

cyanobacterial community composition PCR-DGGE Synechococcus redundancy analysis 

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Copyright information

© The Author(s) 2012

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 2.0 International License (https://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Authors and Affiliations

  • Juan Ling
    • 1
    • 2
    • 3
    • 4
  • YanYing Zhang
    • 1
    • 3
  • JunDe Dong
    • 1
    • 3
    Email author
  • YouShao Wang
    • 2
  • Hui Huang
    • 1
    • 3
  • Lei Chen
    • 1
    • 3
    • 4
  • XiaoFang Huang
    • 1
    • 3
    • 4
  • LiJuan Long
    • 1
  • Si Zhang
    • 1
  1. 1.Key Laboratory of Marine Bio-resourses Sustainable Utilization, South China Sea Institute of OceanologyChinese Academy of SciencesGuangzhouChina
  2. 2.State Key Laboratory of Tropical Oceanography, South China Sea Institute of OceanologyChinese Academy of SciencesGuangzhouChina
  3. 3.National Experiment Station of Tropical Marine BiologyChinese Academy of SciencesSanyaChina
  4. 4.Graduate University of Chinese Academy of SciencesBeijingChina

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