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Comparative study of biochemical and immunological biomarkers in three marine bivalves exposed at a polluted site

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Abstract

A battery of biochemical and immunological biomarkers used for pollution assessment were measured for first time in the clams Venus verrucosa and Callista chione and were compared with those of the mussel Mytilus galloprovincialis, a well-established indicator organism utilized in numerous environmental monitoring programs. Clams and mussel were transplanted at a polluted and a reference site or maintained at the laboratory. Among biochemical biomarkers, acetylcholinesterase did not differ at the polluted site in all species, but there was a significant difference between the mussel and the clams, glutathione S-transferase showed a clear inhibition at the polluted site in all species and a significant difference between the two clams was also indicated, while catalase activities were increased only in V. verrucosa at the polluted site and not in mussel or the other clam. Immunological biomarkers responses were also pronounced at the polluted site. Lysozyme activity was species-dependent whereas respiratory burst activity measured as luminol-dependent chemiluminescence (CL) was site and stimulus dependent, and it was evident in M. galloprovincialis and V. verrucosa and not in C. chione. Further investigation focused on biochemical and immunological biomarkers related with the oxidative mechanisms in clams will strengthen and expand their use as bioindicators for pollution assessment.

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Acknowledgments

This study was partially supported by the General Secretariat of Research and Technology (Greece) in the framework of the Joint Research and Technology Programmes (Greece-Tunisia 2005–2008).

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  1. Institute of Aquaculture, Hellenic Centre for Marine Research (HCMR), Agios Kosmas, 16777, Ellinikon, Greece

    Efthimia Cotou & Morgane Henry

  2. Institute of Oceanography, Hellenic Centre for Marine Research (HCMR), 46.7 km Athinon-Souniou, 19013, Anavissos, Greece

    Catherine Tsangaris

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  1. Efthimia Cotou
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Correspondence to Efthimia Cotou.

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Cotou, E., Tsangaris, C. & Henry, M. Comparative study of biochemical and immunological biomarkers in three marine bivalves exposed at a polluted site. Environ Sci Pollut Res 20, 1812–1822 (2013). https://doi.org/10.1007/s11356-012-1150-3

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