Abstract
In this work, the biosynthesis of silver nanoparticles by Galega officinalis extract using AgNO3 as a precursor was reported. The reaction parameters for the biosynthesis and efficiency in their antimicrobial control against Escherichia coli, Staphylococcus aureus and Pseudomonas syringae were determined. For biosynthesis, a central composite design combined with response surface methodology was used to optimize the process parameters (pH, AgNO3 and extract concentration), and the design was assessed through the size distribution, zeta potential and polydispersity index of the nanoparticles. The results demonstrated that at pH 11, 1.6 mM of AgNO3 and 15% vv−1 of G. officinalis extract were the optimal reaction parameters. Transmission electron microscope (TEM) images and X-ray diffraction (XRD) confirmed the formation of small spherical silver nanoparticles. Antimicrobial assays showed a high inhibitory effect against E. coli, S. aureus and P. syringae, and that effect was larger with silver nanoparticles of a smaller size (23 nm). This work demonstrates that G. officinalis extract is a feasible medium for the synthesis of silver nanoparticles and that the control of the reaction parameters can determine the nanoparticle characteristics and therefore their antimicrobial effectiveness.
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Acknowledgements
This works was financed by FONDECYT (Project Nos. 1130854 and 1161713), REDES-CONICYT 180003, MEC-CONICYT 80170089 and 80170096, CONICYT/FONDAP/15130015 and Vicerrectoría de Investigación y Postgrado UFRO DI17-1002 and DI17-2015.
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Manosalva, N., Tortella, G., Cristina Diez, M. et al. Green synthesis of silver nanoparticles: effect of synthesis reaction parameters on antimicrobial activity. World J Microbiol Biotechnol 35, 88 (2019). https://doi.org/10.1007/s11274-019-2664-3
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DOI: https://doi.org/10.1007/s11274-019-2664-3