World Journal of Microbiology and Biotechnology

, Volume 26, Issue 12, pp 2291–2295 | Cite as

Bioemulsifier production by Aspergillus niger MYA 135: presumptive role of iron and phosphate on emulsifying ability

  • Verónica Leticia Colin
  • Mario Domingo Baigorí
  • Licia María Pera
Short Communication

Abstract

Microbial emulsifiers are compounds employed in primary mechanisms for bioremediation of petroleum and other hydrocarbon pollutants from the environment. Although emulsifiers of biological origin are produced by microorganisms generally in response to growth on hydrocarbons, Aspergillus niger MYA 135 produced a bioemulsifier during fermentation in a sucrose-based culture medium at an initial pH of 5.0 and at 30°C. The production of bioemulsifiers can be strongly influenced by environmental factors. In this connection, a study of the effect of initial pH, the incubation temperature and presence of CaCl2 or FeCl3 in the culture medium was conducted. Emulsification index was increased by 112 and 206% at an initial pH 2.0 or in medium supplemented with FeCl3, respectively. On the other hand, emulsifying ability of Aspergillus niger supernatants was detected during the exponential phase, suggesting that bioemulsifiers accumulation and microbial growth would be related. Interestingly, this study suggests that iron and/or phosphate ions would play a key role in maintaining the emulsifying ability. Finally, factorial design was also employed to study the effects of the initial pH, the presence of FeCl3 and the concentration of KH2PO4 on the emulsification index.

Keywords

Aspergillus niger Bioemulsifier accumulation Emulsification index Factorial design 

Notes

Acknowledgments

This work was partially supported by grants Resolution Nº 693/04 from the Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET) and CIUNT 26/D 409.

References

  1. Amaral PFF, da Silva JM, Lehocky M, Barros-Timmons AMV, Coelho MAZ, Marrucho LM, Coutinho JAO (2006) Production and characterization of a bioemulsifier from Yarrowia lipolytica. Process Biochem 41:1894–1898CrossRefGoogle Scholar
  2. Bosch MP, Robert M, Mercade ME, Espuny MJ, Parra JL, Guinea J (1988) Surface-active compounds on microbial cultures. Tenside Surfactants Deterg 25:208–211Google Scholar
  3. Calvo C, Manzanera M, Silva-Castro GA, Uad I, González-López J (2009) Application of bioemulsifiers in soil oil bioremediation processes. Future prospects. Sci Total Environ 407:3634–3640CrossRefGoogle Scholar
  4. Colin VL, Baigori MD, Pera LM (2010) Effect of environmental conditions on extracellular lipases production and fungal morphology from Aspergillus niger MYA 135. J Basic Microbiol 50:52–58CrossRefGoogle Scholar
  5. Cooper DG, Goldenberg BG (1987) Surface-active agents from two Bacillus species. Appl Environ Microbiol 53:224–229Google Scholar
  6. Gutierrez T, Shimmield T, Haidon C, Black K, Green DH (2008) Emulsifying and metal ion binding activity of a glycoprotein exopolimer produced by Pseudoalteromonas sp. strain TG12. Appl Environ Microbiol 15:4867–4876CrossRefGoogle Scholar
  7. Luna-Velasco MA, Esparza-García F, Cañizares-Villanueva RO, Rodríguez-Vázquez R (2007) Production and properties of a bioemulsifier synthesized by phenanthrene-degrading Penicillium sp. Process Biochem 42:310–314CrossRefGoogle Scholar
  8. Martínez-Checa F, Toledo FL, El Mabrouki K, Quesada E, Calvo C (2007) Characteristics of Bioemulsifier V2–7 synthesized in culture media added of hydrocarbons: chemical composition, emulsifying activity and rheological properties. Biotechnol Resour 98:3130–3135Google Scholar
  9. Miller GL (1969) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31:426–428CrossRefGoogle Scholar
  10. Pera LM, Callieri DA (1997) Influence of calcium on fungal growth, hyphal morphology and citric acid production in Aspergillus niger. Folia Microbiol 42:551–556CrossRefGoogle Scholar
  11. Zinjarde SS, Pant A (2002) Emulsifier from a tropical marine yeast, Yarrowia lipolytica NCIM 3589. J Basic Microbiol 42:67–73CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • Verónica Leticia Colin
    • 1
  • Mario Domingo Baigorí
    • 1
  • Licia María Pera
    • 1
  1. 1.PROIMI-CONICETTucumánArgentina

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