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Alkali-thermostable and cellulase-free xylanase production by an extreme thermophile Geobacillus thermoleovorans

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Abstract

The optimization of cultural variables resulted in a marked enhancement in the secretion of cellulase-free and alkali-thermostable xylanase (EC 3.2.1.8) by an extreme thermophile Geobacillus thermoleovorans. The enzyme secretion was enhanced when the medium was supplemented with xylan (0.15%) and Tween-80 (0.1% v/v). In wheat bran-tryptone medium, the peak in enzyme production was attained within 42 h in a fermenter as compared to 72 h in shake flasks. Optimization of the culture conditions resulted in a 7.72-fold enhancement in enzyme production. The cellulase-free xylanase was optimally active at pH 8.5 and 80°C, and it was found to be useful in the pre-bleaching process of paper pulps.

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Acknowledgements

AS and SK are grateful to the University Grants Commission (UGC), Government of India for providing financial assistance during the course of this investigation.

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  1. Department of Microbiology, University of Delhi South Campus, Benito Juarez Road, New Delhi, 110 021, India

    A. Sharma, S. Adhikari & T. Satyanarayana

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  1. A. Sharma
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  2. S. Adhikari
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  3. T. Satyanarayana
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Correspondence to T. Satyanarayana.

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Sharma, A., Adhikari, S. & Satyanarayana, T. Alkali-thermostable and cellulase-free xylanase production by an extreme thermophile Geobacillus thermoleovorans . World J Microbiol Biotechnol 23, 483–490 (2007). https://doi.org/10.1007/s11274-006-9250-1

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