Alkali-thermostable and cellulase-free xylanase production by an extreme thermophile Geobacillus thermoleovorans

Original Paper

Abstract

The optimization of cultural variables resulted in a marked enhancement in the secretion of cellulase-free and alkali-thermostable xylanase (EC 3.2.1.8) by an extreme thermophile Geobacillus thermoleovorans. The enzyme secretion was enhanced when the medium was supplemented with xylan (0.15%) and Tween-80 (0.1% v/v). In wheat bran-tryptone medium, the peak in enzyme production was attained within 42 h in a fermenter as compared to 72 h in shake flasks. Optimization of the culture conditions resulted in a 7.72-fold enhancement in enzyme production. The cellulase-free xylanase was optimally active at pH 8.5 and 80°C, and it was found to be useful in the pre-bleaching process of paper pulps.

Keywords

Cellulase-free xylanase Extreme thermophile Geobacillus thermoleovorans Optimization Paper pulp Pre-bleaching 

Notes

Acknowledgements

AS and SK are grateful to the University Grants Commission (UGC), Government of India for providing financial assistance during the course of this investigation.

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Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  1. 1.Department of MicrobiologyUniversity of Delhi South CampusNew DelhiIndia

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