Virus Genes

, Volume 55, Issue 1, pp 117–121 | Cite as

Air potato (Dioscorea bulbifera) plants displaying virus-like symptoms are co-infected with a novel potyvirus and a novel ampelovirus

  • Kishore K. Dey
  • Jaylinn Sugikawa
  • Christopher Kerr
  • Michael J. MelzerEmail author


Air potato (Dioscorea bulbifera) plants being grown at the Florida Department of Agriculture and Consumer Services Division of Plant Industry Biological Control Laboratory II in Alachua County, Florida were observed exhibiting foliar mosaic symptoms characteristic of virus infection. A double-stranded RNA library generated from a symptomatic plant underwent high-throughput sequencing to determine if viral pathogens were present. Sequence data revealed the presence of two viral genomes, one with properties congruent with members of the genus Potyvirus (family Potyviridae), and the other with members of the genus Ampelovirus (family Closteroviridae). Sequence comparisons and phylogenetic placement indicate that both viruses represent novel species. The names “dioscorea mosaic virus” and “air potato virus 1” are proposed for the potyvirus and ampelovirus, respectively.


Air potato Ampelovirus Potyvirus High-throughput sequencing Genome characterization Plant virus 



The work in Hawaii was supported by the USDA National Institute of Food and Agriculture Hatch Project HAW09030-H awarded to M.J. Melzer and managed by the University of Hawaii’s College of Tropical Agriculture and Human Resources.

Compliance with ethical standards

Conflict of interest

The authors declare that there is no conflicts of interest associated with this study.

Research involving human and animal participants

This article does not contain any studies with human participants or animals performed by any of the authors.


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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Division of Plant IndustryFlorida Department of Agriculture and Consumer ServicesGainesvilleUSA
  2. 2.Department of Plant and Environmental Protection SciencesUniversity of HawaiiHonoluluUSA

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