Abstract
A total of 575 commercial grapevine (Vitis vinifera) samples from Australia have been tested for a newly emerging virus, Grapevine Pinot gris virus (Trichovirus, Betaflexiviridae) during 2015–2017. Nine samples from two states tested positive. Six of these were from New South Wales and 3 from South Australia in a total of eight varieties. All these varieties were imported from Europe within the last 4–19 years. A fragment of 431 nucleotides on the coat protein gene of Grapevine Pinot gris virus was targeted for virus identification by RT-PCR. The virus specificity of each positive sample was confirmed by sequencing followed by the BLASTn analysis which showed an identity of up to 99.3% to the virus sequences in the NCBI database. The phylogenetic tree as well as pairwise sequence identity showed that, although the virus sequence in each variety was unique, they all grouped with the isolates from Europe and well away from the South Korean and several Chinese isolates. This is the first report of the occurrence of Grapevine Pinot gris virus in Australia.
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Acknowledgements
The sequence of the PCR primers was kindly provided by E. Angelini (Italy) before publication.
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Neither Nuredin Habili, nor my co-author, Ms Qi Wu has any conflict of interest with this work. Qi Wu did most of the work and Nuredin Habili did the analysis and writing up.
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Edited by Seung-Kook Choi.
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Wu, Q., Habili, N. The recent importation of Grapevine Pinot gris virus into Australia. Virus Genes 53, 935–938 (2017). https://doi.org/10.1007/s11262-017-1475-6
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DOI: https://doi.org/10.1007/s11262-017-1475-6