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Identification of herbaceous hosts of the Grapevine Pinot gris virus (GPGV)

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Abstract

Grapevine Pinot gris Virus (GPGV) is a single stranded RNA of the genus Trichovirus infecting grapevine (Vitis vinifera) and associated with stunting, chlorotic mottling and leaf deformation symptoms. During a monitoring of GPGV infection in vineyards of the Trentino region in Italy, we have detected the virus in the herbaceous plants Silene latifolia subsp. Alba (Mill.) (bladder campion) and Chenopodium album L. (white goosefoot), which showed symptoms of viral infection. The full-length GPGV RNA genome, amplified from these infected hosts, was sequenced and a phylogenetic analysis revealed that its closest relative is the strain SK13, recently isolated in Slovakia. Our results indicate that herbaceous plants can be considered as a reservoir for the GPGV virus. This finding is important for studying the epidemiological aspects of GPGV disease and to formulate appropriate control measures.

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Fig. 1
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Abbreviations

aa:

Amino Acids

CP:

Coat Protein

dsRNA:

Double stranded RNA

GPGV:

Grapevine Pinot gris Virus

kDa:

Kilo Daltons

MP:

Movement protein

ORF:

Open reading frame

PCR:

Polymerase chain reaction

ssRNA:

Single stranded RNA

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Acknowledgments

This research was supported by “Progetto di ricerca per una nuova malattia della vite in Trentino” funded by FEM-IASMA, Centre for Technology Transfer, via E. Mach,1 San Michele all’ Adige (TN), Italy.

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Authors and Affiliations

  1. Fondazione Edmund Mach, Via E. Mach 1, 38010, San Michele All’Adige (TN), Italy

    Valeria Gualandri, Elisa Asquini, Pierluigi Bianchedi, Laura Covelli, Matteo Brilli, Umberto Malossini, Paola Bragagna & Azeddine Si-Ammour

  2. CNR Istituto per la protezione sostenibile delle piante, UOS-Bari, via Amendola 165/A, 70126, Bari, Italy

    Pasquale Saldarelli

Authors
  1. Valeria Gualandri
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  2. Elisa Asquini
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  3. Pierluigi Bianchedi
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  4. Laura Covelli
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  5. Matteo Brilli
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  6. Umberto Malossini
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  7. Paola Bragagna
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  8. Pasquale Saldarelli
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  9. Azeddine Si-Ammour
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Corresponding author

Correspondence to Azeddine Si-Ammour.

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Gualandri, V., Asquini, E., Bianchedi, P. et al. Identification of herbaceous hosts of the Grapevine Pinot gris virus (GPGV). Eur J Plant Pathol 147, 21–25 (2017). https://doi.org/10.1007/s10658-016-0989-4

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  • DOI: https://doi.org/10.1007/s10658-016-0989-4

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