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Molecular genotyping of Mycobacterium bovis isolated from cattle tissues in the North West Region of Cameroon

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Abstract

An epidemiological study was carried out to determine the Mycobacterium bovis strains causing bovine tuberculosis (TB) in cattle in North West Cameroon. Suspected TB lesions from slaughtered cattle were cultured on Lowenstein–Jensen and Middlebrook 7 H9 media to isolate mycobacteria agents for molecular genotyping using deletion analysis and spoligotyping. PCR-based genomic deletion typing showed that 54 of 103 tubercle bacilli isolated from cattle tissue were M. bovis strains and the African 1 clonal complex was widespread in affected cattle. Spoligotyping analysis revealed a closely related group of five M. bovis strains. SB0953, the dominant spoligotype pattern, and four new patterns identified as SB2161, SB2162, SB2663 and SB2664 according to the www.Mbovis.org international spoligotype database were identified. These spoligotypes were similar to other M. bovis strains recovered from bordering regions and other parts of Africa. The findings provided useful facts on the zoonotic risks of bovine TB and overwhelming evidence of the significance of M. bovis infection to human TB in the North West Region of Cameroon. The study revealed that bovine TB was widespread in cattle destined for human consumption and also has important implications for the control of TB in animals and humans in Cameroon.

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Acknowledgments

This study was supported by a PhD grant of the Commonwealth Scholarship Commission in UK. NH Smith was supported by the Department of Environment, Food and Rural Affairs, UK (project SB4020). The authors are also grateful to the veterinary staff for allowing the collection of samples and other abattoir workers for their generous cooperation.

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Authors and Affiliations

  1. Department of Animal Sciences, University of Dschang, Dschang, Cameroon

    Julius Awah-Ndukum

  2. School of Veterinary Medicine and Sciences, University of Ngaoundere, Ngaoundéré, Cameroon

    Julius Awah-Ndukum

  3. School of Biomedical and Biological Sciences, University of Plymouth, Plymouth, UK

    Julius Awah-Ndukum, Ayuba Caleb Kudi & Graham Bradley

  4. Department of Veterinary Medicine, Ahmadu Bello University, Zaria, Nigeria

    Ayuba Caleb Kudi

  5. Animal Health and Veterinary Laboratories Agency, Weybridge, Surrey, UK

    Noel H. Smith

  6. Department of Biochemistry and Microbiology, University of Buea, Buea, Cameroon

    Irene Ane-Anyangwe, Fidelis Cho-Ngwa & Vicent Pryde Kehdinga Titanji

Authors
  1. Julius Awah-Ndukum
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  2. Ayuba Caleb Kudi
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  3. Graham Bradley
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  4. Noel H. Smith
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  5. Irene Ane-Anyangwe
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  6. Fidelis Cho-Ngwa
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  7. Vicent Pryde Kehdinga Titanji
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Correspondence to Julius Awah-Ndukum.

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Awah-Ndukum, J., Kudi, A.C., Bradley, G. et al. Molecular genotyping of Mycobacterium bovis isolated from cattle tissues in the North West Region of Cameroon. Trop Anim Health Prod 45, 829–836 (2013). https://doi.org/10.1007/s11250-012-0295-x

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  • DOI: https://doi.org/10.1007/s11250-012-0295-x

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