Tropical Animal Health and Production

, Volume 45, Issue 3, pp 829–836

Molecular genotyping of Mycobacterium bovis isolated from cattle tissues in the North West Region of Cameroon

  • Julius Awah-Ndukum
  • Ayuba Caleb Kudi
  • Graham Bradley
  • Noel H. Smith
  • Irene Ane-Anyangwe
  • Fidelis Cho-Ngwa
  • Vicent Pryde Kehdinga Titanji
Regular Articles

Abstract

An epidemiological study was carried out to determine the Mycobacterium bovis strains causing bovine tuberculosis (TB) in cattle in North West Cameroon. Suspected TB lesions from slaughtered cattle were cultured on Lowenstein–Jensen and Middlebrook 7 H9 media to isolate mycobacteria agents for molecular genotyping using deletion analysis and spoligotyping. PCR-based genomic deletion typing showed that 54 of 103 tubercle bacilli isolated from cattle tissue were M. bovis strains and the African 1 clonal complex was widespread in affected cattle. Spoligotyping analysis revealed a closely related group of five M. bovis strains. SB0953, the dominant spoligotype pattern, and four new patterns identified as SB2161, SB2162, SB2663 and SB2664 according to the www.Mbovis.org international spoligotype database were identified. These spoligotypes were similar to other M. bovis strains recovered from bordering regions and other parts of Africa. The findings provided useful facts on the zoonotic risks of bovine TB and overwhelming evidence of the significance of M. bovis infection to human TB in the North West Region of Cameroon. The study revealed that bovine TB was widespread in cattle destined for human consumption and also has important implications for the control of TB in animals and humans in Cameroon.

Keywords

Cattle Tuberculous lesions Mycobacterium bovis Molecular genotyping Zoonosis North West Cameroon 

References

  1. Ameni, G., Desta, F., Firdessa, R., 2010. Molecular typing of Mycobacterium bovis isolated from tuberculosis lesions of cattle in north eastern Ethiopia. Veterinary Record 167, 138-141.PubMedCrossRefGoogle Scholar
  2. AU/IBAR, 2006. Pan African Animal Health Yearbook 2006. African Union/Inter African Bureau for Animal Resources Nairobi, Kenya, 84.Google Scholar
  3. Awah-Ndukum, J., Kudi, A.C., Bradley, G., Ane-Anyangwe, I.N., Fon-Tebug, S., Tchoumboue, J., 2010. Prevalence of bovine tuberculosis in abattoirs of the Littoral and Western highland regions of Cameroon: a cause for public health concern. Veterinary Medicine International 2010, 8 pages; doi:10.4061/2010/495015.
  4. Awah-Ndukum, J., Kudi, A.C., Bah, G.S., Bradley, G., Tebug, S.F., Dickmu, P.L., Njakoi, H.N., Agharih, W.N., 2012a. Bovine Tuberculosis in Cattle in the Highlands of Cameroon: Seroprevalence Estimates and Rates of Tuberculin Skin Test Reactors at Modified Cut-Offs. Veterinary Medicine International 2012 13 pages. doi:10.1155/2012/798502.
  5. Awah-Ndukum, J., Kudi, A.C., Bradley, G., Ane-Anyangwe, I., Titanji, V.P.K., Fon-Tebug, S., Tchoumboue, J., 2012b. Prevalence of bovine tuberculosis in cattle in the highlands of Cameroon based on the detection of lesions in slaughtered cattle and tuberculin skin tests of live cattle. Veterinarni Medicina 57, 59–76.Google Scholar
  6. Ayele, W.Y., Neill, S.D., Zinsstag, J., Weiss, M.G., Pavlik, I., 2004. Bovine tuberculosis: an old disease but a new threat to Africa. The International Journal of Tuberculosis and Lung Disease 8, 924-937.PubMedGoogle Scholar
  7. Brosch, R., Gordon, S.V., Marmiesse, M., Brodin, P., Buchrieser, C., Eiglmeier, K., Garnier, T., Gutierrez, C., Hewinson, G., Kremer, K., Parsons, L.M., Pym, A.S., Samper, S., van Soolingen, D., Cole, S.T., 2002. A new evolutionary scenario for the Mycobacterium tuberculosis complex. Proceedings of the National Academy of Sciences of the United States of America 99, 3684-3689.PubMedCrossRefGoogle Scholar
  8. Cadmus, S., Palmer, S., Okker, M., Dale, J., Gover, K., Smith, N., Jahans, K., Hewinson, R.G., Gordon, S.V., 2006. Molecular Analysis of Human and Bovine Tubercle Bacilli from a Local Setting in Nigeria. Journal of Clinical Microbiology 44, 29-34.PubMedCrossRefGoogle Scholar
  9. Diguimbaye-Djaibé, C., Hilty, M., Ngandolo, R., Mahamat, H.H., Pfyffer, G.E., Baggi, F., Hewinson, G., Tanner, M., Zinsstag, J., Schelling, E., 2006. Mycobacterium bovis Isolates from Tuberculous Lesions in Chadian Zebu Carcasses. Emerging Infectious Diseases 12, 769-771.PubMedCrossRefGoogle Scholar
  10. FAO, 1994. Manual on meat inspection for developing countries. In: Herenda, D., Chambers, P.G., Ettriqui, A., Seneviratna, P., da Silva, T.J.P. (Eds.), FAO Animal Production and Health Paper 119. Food and Agriculture Organization of the United Nations, Rome, Italy, 388.Google Scholar
  11. Gibson, A.L., Hewinson, G., Goodchild, T., Watt, B., Story, A., Inwald, J., Drobniewski, F.A., 2004. Molecular Epidemiology of Disease Due to Mycobacterium bovis in Humans in the United Kingdom. Journal of Clinical Microbiology 42, 431-434.PubMedCrossRefGoogle Scholar
  12. Grist, A., 2008. Bovine Meat Inspection—Anatomy, Physiology and Disease Conditions. Nottingham University Press, Nottingham; 296.Google Scholar
  13. Kamerbeek, J., Schouls, L., Kolk, A., van Agterveld, M., van Soolingen, D., Kuijper, S., Bunschoten, A., Molhuizen, H., Shaw, R., Goyal, M., van Embden, J., 1997. Simultaneous detection and strain differentiation of Mycobacterium tuberculosis for diagnosis and epidemiology. Journal of Clinical Microbiology 35, 907–914.PubMedGoogle Scholar
  14. Kazwala, R.R., Kusiluka, L.J.M., Sinclair, K., Sharp, J.M., Daborn, C.J., 2006. The molecular epidemiology of Mycobacterium bovis infections in Tanzania. Veterinary Microbiology 112, 201-210.PubMedCrossRefGoogle Scholar
  15. MINEPIA, 2002. La stratégie sectoriel de l’élevage, des peches et industries animales. In: Doufissa, A. (Ed.) Cabinet Management 2000 MINEPIA. Ministry of Livestock, Fisheries and Animal Industries, Yaounde, Cameroon.Google Scholar
  16. Müller, B., Steiner, B., Bonfoh, B., Fane, A., Smith, N., Zinsstag, J., 2008. Molecular characterisation of Mycobacterium bovis isolated from cattle slaughtered at the Bamako abattoir in Mali. BMC Veterinary Research 4, 26.PubMedCrossRefGoogle Scholar
  17. Müller, B., Hilty, M., Berg, S., Garcia-Pelayo, M.C., Dale, J., Boschiroli, M.L., Cadmus, S., Ngandolo, B.N.R., Godreuil, S., Diguimbaye-Djaibe, C., Kazwala, R., Bonfoh, B., Njanpop-Lafourcade, B.M., Sahraoui, N., Guetarni, D., Aseffa, A., Mekonnen, M.H., Razanamparany, V.R., Ramarokoto, H., Djonne, B., Oloya, J., Machado, A., Mucavele, C., Skjerve, E., Portaels, F., Rigouts, L., Michel, A., Muller, A., Kallenius, G., van Helden, P.D., Hewinson, R.G., Zinsstag, J., Gordon, S.V., Smith, N.H., 2009. African 1, an Epidemiologically Important Clonal Complex of Mycobacterium bovis Dominant in Mali, Nigeria, Cameroon, and Chad. Journal of Bacteriology 191, 1951-1960.PubMedCrossRefGoogle Scholar
  18. Niobe-Eyangoh, S.N., Kuaban, C., Sorlin, P., Cunin, P., Thonnon, J., Sola, C., Rastogi, N., Vicent, V., Gutierrez, M.C., 2003. Genetic biodiversity of Mycobacterium tuberculosis complex strains from patients with pulmonary tuberculosis in Cameroon. Journal of Clinical Microbiology 41, 2547-2553.PubMedCrossRefGoogle Scholar
  19. Njanpop-Lafourcade, B.M., Inwald, J., Ostyn, A., Durand, B., Hughes, S., Thorel, M.-F., Hewinson, G., Haddad, N., 2001. Molecular Typing of Mycobacterium bovis Isolates from Cameroon. Journal of Clinical Microbiology 39, 222-227.PubMedCrossRefGoogle Scholar
  20. Noeske, J., Kuaban, C., Cunin, P., 2004. Are smear-positive pulmonary tuberculosis patients a ‘sentinel’ population for the HIV epidemic in Cameroon? The International Journal of Tuberculosis and Lung Disease 8, 346-351.PubMedGoogle Scholar
  21. Parsons, L.M., Brosch, R., Cole, S.T., Somoskövi, Á., Loder, A., Bretzel, G., van Soolingen, D., Hale, Y.M., Salfinger, M., 2002. Rapid and Simple Approach for Identification of Mycobacterium tuberculosis Complex Isolates by PCR-Based Genomic Deletion Analysis. Journal of Clinical Microbiology 40, 2339-2345PubMedCrossRefGoogle Scholar
  22. Pinsky, B.A., Banaei, N., 2008. Multiplex Real-Time PCR assay for rapid identification of Mycobacterium tuberculosis complex members to the species Level. Journal of Clinical Microbiology 46, 2241–2246.PubMedCrossRefGoogle Scholar
  23. Pollock, J.M., Neill, S.D., 2002. Mycobacterium bovis Infection and Tuberculosis in Cattle. The Veterinary Journal 163, 115-127.PubMedCrossRefGoogle Scholar
  24. Smith, N.H., Upton, P., 2012. Naming spoligotype patterns for the RD9-deleted lineage of the Mycobacterium tuberculosis complex; www.Mbovis.org. Infection, Genetics and Evolution 12, 873-876.CrossRefGoogle Scholar
  25. Warren, R.M., Pittius, N.C.G.v., Barnard, M., Hesseling, A., Engelke, E., Kock, M.d., Gutierrez, M.C., Chege, G.K., Victor, T.C., Hoal, E.G., Helden, P.D.v., 2006. Differentiation of Mycobacterium tuberculosis complex by PCR amplification of genomic regions of difference. The International Journal of Tuberculosis and Lung Disease 10, 818-822.PubMedGoogle Scholar
  26. Whipple, D.L., Bolin, C.A., Miller, J.M., 1996. Distribution of lesions in cattle infected with Mycobacterium bovis. Journal of Veterinary Diagnostic Investigation 8, 351-354.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Julius Awah-Ndukum
    • 1
    • 2
    • 3
  • Ayuba Caleb Kudi
    • 3
    • 4
  • Graham Bradley
    • 3
  • Noel H. Smith
    • 5
  • Irene Ane-Anyangwe
    • 6
  • Fidelis Cho-Ngwa
    • 6
  • Vicent Pryde Kehdinga Titanji
    • 6
  1. 1.Department of Animal SciencesUniversity of DschangDschangCameroon
  2. 2.School of Veterinary Medicine and Sciences, University of NgaoundereNgaoundéréCameroon
  3. 3.School of Biomedical and Biological SciencesUniversity of PlymouthPlymouthUK
  4. 4.Department of Veterinary MedicineAhmadu Bello UniversityZariaNigeria
  5. 5.Animal Health and Veterinary Laboratories AgencyWeybridgeUK
  6. 6.Department of Biochemistry and Microbiology, University of BueaBueaCameroon

Personalised recommendations