Tropical Animal Health and Production

, Volume 45, Issue 3, pp 829–836

Molecular genotyping of Mycobacterium bovis isolated from cattle tissues in the North West Region of Cameroon

  • Julius Awah-Ndukum
  • Ayuba Caleb Kudi
  • Graham Bradley
  • Noel H. Smith
  • Irene Ane-Anyangwe
  • Fidelis Cho-Ngwa
  • Vicent Pryde Kehdinga Titanji
Regular Articles


An epidemiological study was carried out to determine the Mycobacterium bovis strains causing bovine tuberculosis (TB) in cattle in North West Cameroon. Suspected TB lesions from slaughtered cattle were cultured on Lowenstein–Jensen and Middlebrook 7 H9 media to isolate mycobacteria agents for molecular genotyping using deletion analysis and spoligotyping. PCR-based genomic deletion typing showed that 54 of 103 tubercle bacilli isolated from cattle tissue were M. bovis strains and the African 1 clonal complex was widespread in affected cattle. Spoligotyping analysis revealed a closely related group of five M. bovis strains. SB0953, the dominant spoligotype pattern, and four new patterns identified as SB2161, SB2162, SB2663 and SB2664 according to the international spoligotype database were identified. These spoligotypes were similar to other M. bovis strains recovered from bordering regions and other parts of Africa. The findings provided useful facts on the zoonotic risks of bovine TB and overwhelming evidence of the significance of M. bovis infection to human TB in the North West Region of Cameroon. The study revealed that bovine TB was widespread in cattle destined for human consumption and also has important implications for the control of TB in animals and humans in Cameroon.


Cattle Tuberculous lesions Mycobacterium bovis Molecular genotyping Zoonosis North West Cameroon 


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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Julius Awah-Ndukum
    • 1
    • 2
    • 3
  • Ayuba Caleb Kudi
    • 3
    • 4
  • Graham Bradley
    • 3
  • Noel H. Smith
    • 5
  • Irene Ane-Anyangwe
    • 6
  • Fidelis Cho-Ngwa
    • 6
  • Vicent Pryde Kehdinga Titanji
    • 6
  1. 1.Department of Animal SciencesUniversity of DschangDschangCameroon
  2. 2.School of Veterinary Medicine and Sciences, University of NgaoundereNgaoundéréCameroon
  3. 3.School of Biomedical and Biological SciencesUniversity of PlymouthPlymouthUK
  4. 4.Department of Veterinary MedicineAhmadu Bello UniversityZariaNigeria
  5. 5.Animal Health and Veterinary Laboratories AgencyWeybridgeUK
  6. 6.Department of Biochemistry and Microbiology, University of BueaBueaCameroon

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