Kinetic and regulatory properties of plant ADP-glucose pyrophosphorylase genetically modified by heterologous expression of potato upreg mutants in vitro and in vivo

  • Si-Myung Lee
  • Tae-Hun Ryu
  • Su-Il Kim
  • Thomas Okita
  • Donghern KimEmail author
Original Paper


In this study, the uses of the mutated genes, upreg1 and upreg2, encoding upregulated ADP-glucose pyrophosphorylase (AGPase) large subunits with increased enzymatic activity, to improve crop yield productivity was evaluated in vitro and in planta. For in vitro examination, wild type and upregs were co-expressed with three different AGPase small subunit genes from potato and perilla to produce nine AGPase isoforms. In kinetic experiments, 3-Phosphoglycerate increased the V max and decreased the K M for the recombinant AGPase. Regardless of the specific small subunit, Upreg-type AGPases had much larger increases in enzymatic activity with concomitant decreases in values as compared to the wild type enzyme. Transformation of lettuce with the upreg1 gene altered the regulatory properties of leaf AGPase. AGPases from transgenic lettuce showed greater 3-PGA activation and lower Pi inhibition than was observed for wild type AGPase. Fresh weights of the aerial parts of transgenic plants were larger than non-transgenic controls. Based on these results, upreg mutant genes could be used for the genetic improvement of plant AGPases other than potato and effectively increase crop yield productivity.


ADP-glucose pyrophosphorylase Lettuce Upreg 





ADP-glucose pyrophosphorylase









This research was supported by National Institute of agricultural Biotechnology, RDA (05-5-11-22-3).


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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Si-Myung Lee
    • 1
  • Tae-Hun Ryu
    • 1
  • Su-Il Kim
    • 2
  • Thomas Okita
    • 3
  • Donghern Kim
    • 1
    Email author
  1. 1.Biosafety DivisionNational Institute of Agricultural Biotechnology, Rural Development AdministrationSuwonSouth Korea
  2. 2.Department of Agricultural Biotechnology and Center for Agricultural Biomaterials, College of Agriculture and Life SciencesSeoul National UniversitySeoulSouth Korea
  3. 3.Institute of Biological ChemistryWashington State UniversityPullmanUSA

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