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Micrografting of Protea cynaroides

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Abstract

The inability to induce rooting of in vitro-established Protea cynaroides microshoots has prevented the production of complete plantlets. A successful shoot-tip micrografting technique was developed using in vitro-germinated P. cynaroides seedlings as rootstocks and axenic microshoots established from pot plants as microscions. Thirty-day old seedlings, germinated on growth-regulator-free, half-strength Murashige and Skoog medium, were decapitated and a vertical incision made from the top end. The bottom ends of microshoots established on modified Murashige and Skoog medium were cut into a wedge (‘V’) shape, and placed into the incision. The micrografted explants were cultured in a growth chamber with the temperature adjusted to 25 ± 2°C, with a 12-h photoperiod. Best results were obtained by placing the microscions directly onto the rootstock without any pre-treatments. Dipping the explants in anti-oxidant solution or placing a layer of medium around the graft area led to the blackening of the microscion.

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Abbreviations

EDTA:

Ethylenediaminetetraacetate

BAP:

6-Benzylaminopurine

GA3 :

Gibberellic acid

PAR:

Photosynthetic active radiation

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Authors and Affiliations

  1. Department of Plant Production and Soil Science, Faculty of Natural and Agricultural Sciences, University of Pretoria, Pretoria, 0002, South Africa

    H. C. Wu, E. S. du Toit & C. F. Reinhardt

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  1. H. C. Wu
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  2. E. S. du Toit
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  3. C. F. Reinhardt
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Correspondence to E. S. du Toit.

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Wu, H.C., du Toit, E.S. & Reinhardt, C.F. Micrografting of Protea cynaroides . Plant Cell Tiss Organ Cult 89, 23–28 (2007). https://doi.org/10.1007/s11240-007-9208-5

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  • DOI: https://doi.org/10.1007/s11240-007-9208-5

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