Quenching of chlorophyll triplet states by carotenoids in algal light-harvesting complexes related to fucoxanthin-chlorophyll protein

  • Petro Khoroshyy
  • David Bína
  • Zdenko Gardian
  • Radek Litvín
  • Jan Alster
  • Jakub Pšenčík
Original Article
  • 103 Downloads

Abstract

We have used time-resolved absorption and fluorescence spectroscopy with nanosecond resolution to study triplet energy transfer from chlorophylls to carotenoids in a protective process that prevents the formation of reactive singlet oxygen. The light-harvesting complexes studied were isolated from Chromera velia, belonging to a group Alveolata, and Xanthonema debile and Nannochloropsis oceanica, both from Stramenopiles. All three light-harvesting complexes are related to fucoxanthin-chlorophyll protein, but contain only chlorophyll a and no chlorophyll c. In addition, they differ in the carotenoid content. This composition of the complexes allowed us to study the quenching of chlorophyll a triplet states by different carotenoids in a comparable environment. The triplet states of chlorophylls bound to the light-harvesting complexes were quenched by carotenoids with an efficiency close to 100%. Carotenoid triplet states were observed to rise with a ~5 ns lifetime and were spectrally and kinetically homogeneous. The triplet states were formed predominantly on the red-most chlorophylls and were quenched by carotenoids which were further identified or at least spectrally characterized.

Keywords

Algae Energy transfer Light harvesting Photoprotection Photosynthesis Transient spectroscopy 

Supplementary material

11120_2017_416_MOESM1_ESM.pdf (661 kb)
Supplementary material 1 (PDF 662 KB)

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Copyright information

© Springer Science+Business Media B.V. 2017

Authors and Affiliations

  • Petro Khoroshyy
    • 1
  • David Bína
    • 2
    • 3
  • Zdenko Gardian
    • 2
    • 3
  • Radek Litvín
    • 2
    • 3
  • Jan Alster
    • 1
  • Jakub Pšenčík
    • 1
  1. 1.Department of Chemical Physics and Optics, Faculty of Mathematics and PhysicsCharles UniversityPrague 2Czech Republic
  2. 2.Biological CentreCzech Academy of SciencesČeské BudějoviceCzech Republic
  3. 3.Faculty of ScienceUniversity of South BohemiaČeské BudějoviceCzech Republic

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