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Long Non-coding RNA MINCR Regulates miR-876-5p/GSPT1 Axis to Aggravate Glioma Progression

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Abstract

Emerging evidence underlined the crucial roles played by long non-coding RNAs (lncRNAs) in glioma. MINCR has been reported in multiple malignancies. Here, we studied its function and potential mechanism in glioma, which remain unclear. Gene expressions were analyzed by qRT-PCR assay. Both in vitro and in vivo assays were conducted to evaluate the cellular function of MINCR in glioma. The subcellular situation of MINCR was detected by subcellular fractionation and FISH assays. Luciferase reporter, RNA pull-down and RNA immunoprecipitation (RIP) assays were combined to investigate potential mechanisms of relevant genes. MINCR was up-regulated in glioma. MINCR depletion markedly refrained glioma cell proliferation, migration and invasion via sponging miR-876-5p. MiR-876-5p suppressed the malignant behaviors of glioma via binding to GSPT1. MINCR shared the binding sites with the 3’-untranslated region of GSPT1 and prevented the binding of miR-876-5p to GSPT1 mRNA, thus up-regulating the level of GSPT1. Moreover, miR-876 inhibition and GSPT1 up-regulation counteracted the functional effect induced by silencing MINCR on glioma progression. Our findings uncovered that MINCR might aggravated glioma cell proliferation and migration via acting as competing endogenous RNA (ceRNA), indicating prospective novel therapeutic target for glioma.

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Acknowledgements

The current study was supported of all laboratory staff.

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Authors and Affiliations

  1. Department of Neurosurgery, Deyang People’s Hospital, Deyang, 618000, Sichuan, China

    Zheng Li

  2. Department of Emergency, Deyang People’s Hospital, Deyang, 618000, Sichuan, China

    Xiaoxia Xie

  3. General Medical Wards (A), The People’s Hospital of Binzhou City, Binzhou, 25660, Shandong, China

    Xiangyun Fan

  4. Department of Neurosurgery, Taizhou First People’s Hospital, No. 218 Hengjie Road, Dongcheng Street, Huangyan District, Zhejiang, 318020, Taizhou, China

    Xing Li

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Li, Z., Xie, X., Fan, X. et al. Long Non-coding RNA MINCR Regulates miR-876-5p/GSPT1 Axis to Aggravate Glioma Progression. Neurochem Res 45, 1690–1699 (2020). https://doi.org/10.1007/s11064-020-03029-8

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