Abstract
The effects of protein synthesis inhibitors on short-term sensitization of a defensive reaction in common snails and the potentiation of the cholinosensitivity of command neurons were studied. The protein synthesis inhibitor anisomycin did not prevent behavioral sensitization. Anisomycin and the irreversible protein synthesis inhibitor saporin changed the dynamics of potentiation of command neuron cholinosensitivity. We suggest that the sensitization of the defensive response of the common snail studied here does not require the synthesis of new proteins.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
M. S. Abramova, E. I. Drozdova, V. L. Nistratova, and A. S. Pivovarov, “Relationship between posttetanic potentiation of the cholinosensitivity of neurons in the common snail and the humoral factor,” Zh. Vyssh. Nerv. Deyat., 53, No. 5, 533–536 (2003).
M. S. Abramova, V. L. Nistratova, A. A. Moskvitin, and A. S. Pivovarov, “Methiothepin-sensitive serotonin receptors are involved in the postsynaptic mechanism of sensitization of the defensive reaction in the common snail,” Zh. Vyssh. Nerv. Deyat., 55, No. 3, 408–415 (2005).
P. M. Balaban, “Sensitization and acclimation in defensive behavior command neurons in the common snail,” Zh. Vyssh. Nerv. Deyat., 28, No. 2, 356–363 (1978).
Kh. L. Gainutdinov, V. V. Andrianov, and T. Kh. Gainutdinova, Membrane Mechanisms of Behavioral Plasticity During Learning [in Russian], KazFTI, Kazan Scientific Center, Russian Academy of Sciences, Kazan (2002).
T. Kh. Gainutdinova, R. R. Tagirova, A. I. Ismailova, L. N. Muranova, Kh. L. Gainutdinov, and P. M. Balaban, “Protein synthesis-dependent reactivation of a contextual conditioned reflex in the common snail,” Zh. Vyssh. Nerv. Deyat., 54, No. 6, 795–800 (2004).
L. N. Grinkevich, P. D. Lisachev, and M. B. Shtark, “Neurochemical correlates of plasticity,” Zh. Vyssh. Nerv. Deyat., 43, No. 5, 963–968 (1993).
E. Kandel, The Cellular Bases of Behavior [Russian translation] Mir, Moscow (1980).
O. A. Maksimovich and P. M. Balaban, “Neural Mechanisms of Behavioral Plasticity [in Russian], Nauka, Moscow (1983).
A. A. Moskvitin and A. S. Pivovarov, “Apparatus for recording defensive responses in terrestrial snails to tactile stimulation,” Zh. Vyssh. Nerv. Deyat., 53, No. 2, 249–252 (2003).
V. P. Nikitin, “Molecular-cellular mechanisms of learning in the common snail,” Zh. Vyssh. Nerv. Deyat., 43, No. 2, 377–388 (1993).
V. P. Nikitin and S. A. Kozyrev, “Long-term synaptic facilitation in the defensive behavior neuron in snails during acquisition of sensitive depends on RNA synthesis,” Zh. Vyssh. Nerv. Deyat., 54, No. 2, 260–268 (2004).
B. P. Nikitin, S. A. Kozyrev, M. M. Gvozdeva, A. V. Shevelkin, and V. V. Sherstnev, “Protein synthesis inhibitors reproduce the effects of nociceptive sensitization on defensive and food-related responses in the common snail,” Zh. Vyssh. Nerv. Deyat., 44, No. 6, 1004–1015 (1994).
A. S. Pivovarov and I. E. Drozdova, “Dose-dependence of the excitatory effects of acetylcholine on common snail neurons after orthodromic tetanization,” Zh. Vyssh. Nerv. Deyat., 51, No. 1, 117–119 (2001).
A. S. Pivovarov and E. I. Drozdova, “Identification of cholinoreceptors on the body of neurons LPa3 and RPa3 in the common snail,” Neirofiziologiya, 24, No. 1, 77–86 (1992).
A. S. Pivovarov, E. I. Drozdova, and A. A. Moskvitin, “Generalized posttetanic changes in excitatory postsynaptic and acetylcholine-evoked currents in common snail neurons,” Zh. Vyssh. Nerv. Deyat., 49, No. 6, 990-998 (1999).
C. H. Bailey, D. Bartsch, and E. R. Kandel, “Toward a molecular definition of long-term memory storage,” Proc. Natl. Acad. Sci. USA, 26, No. 93, 13445–13452 (1996).
C. H. Bailey, M. Giustetto, H. Zhu, M. Chen, and E. R. Kandel, “A novel function for serotonin-mediated short-term facilitation in Aplysia: conversion of a transient, cell-wide homosynaptic Hebbian plasticity into a persistent, protein synthesis-independent synapsespecific enhancement,” Proc. Natl. Acad. Sci. USA, 97, No. 21, 11591–11586 (2000).
P. M. Balaban, D. A. Poteryaev, I. S. Zakharov, P. Uvarov, A. Malyshev, and A. V. Belyavsky, “Up-and down-regulation of Helix command-specific 2 (HCS2) gene expression in the nervous system of terrestrial snail Helix lucorum,” J. Neurosci., 103, No. 2, 551–559 (2001).
A. Bolognesi, F. Olivieri, L. Battelli, L. Barbieri, A. I. Falasca, A. Parente, F. Del Vecchio Blanco, and F. Stipe, “Ribosome-inactivating proteins (RNA N-glycosidases) from the seeds of Saponaria ocymoides and Vaccaria pyramidata,” Eur. J. Biochem., 228, No. 3, 935–940 (1995).
P. Calabresi, A. Pisani, N. B. Mercuri, and G. Bernardi, “Post-receptor mechanisms underlying striatal long-term depression,” J. Neurosci., 14, No. 8, 4871–4881 (1994).
M. Ghirardi, F. Benfenati, S. Giovedi, F. Fuimara, C. Milanese, and P. G. Montarolo, “Inhibition of neurotransmitter release by a non-physiological target requires protein synthesis and involves cAMP-dependent and mitogen-activated protein kinases,” J. Neurosci., 24, No. 21, 5054–5062 (2004).
M. Ghirardi, P. G. Montarolo, and E. R. Kandel, “A novel intermediate stage in the transition between short-and long-term facilitation in the sensory to motor neuron synapse in Aplysia,” Neuron, 14, No. 2, 413–420 (1995).
R. A. Chitwood, Q. Li, and D. L. Glanzman, “Serotonin facilitates AMPA-type responses in isolated siphon motor neurons of Aplysia in culture,” J. Physiol., 534, No. 2, 501–510 (2001).
J. Mauelshagen, G. R. Parker, and T. J. Carew, “Dynamics of induction and expression of long-term synaptic facilitation in Aplysia,” J. Neurosci., 16, No. 22, 7099–7108 (1996).
P. G. Montarolo, P. Goelet, V. F. Castellucci, J. Morgan, E. R. Kandel, and S. Schacher, “A critical period for macromolecular synthesis in long-term heterosynaptic facilitation in Aplysia,” Science, 234, No. 4781, 1249–1254 (1986).
P. C. Schwindt, W. J. Spain, and W. E. Crill, “Effects of intracellular calcium chelation on voltage-dependent and calcium-dependent currents in cat neocortical neurons,” Neurosci., 47, No. 3, 571–578 (1992).
M. A. Sutton, M. W. Bagnall, S. K. Sharma, J. Shobe, and T. J. Carew, “Intermediate-term memory for site-specific sensitization in Aplysia is maintained by persistent activation of protein kinase C,” J. Neurosci., 24, No. 14, 3600–2609 (2004).
B. Torocsik and J. Szeberenyi, “Anisomycin uses multiple mechanisms to stimulate mitogen-activated protein kinases and gene expression and to inhibit neuronal differentiation in PC12 phaeochromocytoma cells,” Eur. J. Neurosci., 12, No. 2, 527–532 (2000).
L. E. Trudeau and V. Castellucci, “Postsynaptic modifications in long-term facilitation in Aplysia: upregulation of excitatory amino acid receptors,” J. Neurosci., 15, No. 2, 1275–1284 (1995).
M. W. Wood, J. A. Segal, R. J. Mark, A. M. Ogden, and C. C. Felder, “Inflammatory cytokines enhance muscarinic-mediated arachidonic acid release through p38 mitogen-activated protein kinase in A2058 cells,” J. Neurochem., 74, No. 5, 2033–2040 (2000).
Author information
Authors and Affiliations
Additional information
__________
Translated from Zhurnal Vysshei Nervnoi Deyatel’nosti imeni I. P. Pavlova, Vol. 56, No. 3, pp. 355–362, May–June, 2006.
Rights and permissions
About this article
Cite this article
Abramova, M.S., Moskvitin, A.A. & Pivovarov, A.S. Effects of protein synthesis inhibitors on the sensitization of a defensive response in common snails and potentiation of the cholinosensitivity of command neurons. Neurosci Behav Physiol 37, 443–449 (2007). https://doi.org/10.1007/s11055-007-0033-4
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s11055-007-0033-4