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Effect of polymorphism in the peroxisome proliferator-activated receptor gamma gene on litter size of pigs

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Abstract

The association of polymorphisms in peroxisome proliferator-activated receptor γ (PPARγ) gene with litter size was studied in Large White and Landrace pig. Three SNP loci (P1, P2 and P7) on PPARγ2 gene were determined by PCR–SSCP and the results showed that there were A → G mutations at 220 and 324 bp in 5′-regulator region and at 147 bp in exon 6, respectively. Allele frequencies were analysed in two breeds. Information on 2341 litter records from 564 sows was used to analyse the trait total number born (TNB) and number born alive (NBA). In Large White, TNB and NBA of genotype BB for P2 locus were the lowest, and the TNB and NBA of third and following parities and all parities were 0.74 and 0.51 piglets per litter less (P < 0.001) than those of the highest genotype AB, respectively, but for P1 and P7 locus the beneficial genotype AA were more 0.4–0.8 piglets per litter (P < 0.05) than the inferior genotype AB. In landrace, TNB and NBA of the first parity of genotype BB for P1 locus were 2.0 piglets per litter higher than AA (P < 0.05), but for all parities the TNB and NBA of genotype BB were 0.66 and 0.97 piglets per litter (P < 0.05) higher than AA, respectively. At P2 locus, the TNB and NBA of the second parity of genotype AA were obviously higher than those of AB (P < 0.05). And at P7 locus, the TNB and NBA of each parity of genotype AA were both about 2 piglets per litter more than those of BB (P < 0.05). The results indicated that PPARγ gene was significantly associated with litter size in pigs.

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Acknowledgments

The authors greatly appreciate Beijing Huadu Swine Breeding Company Limited and Beijing Breeding Swine Center for providing samples. This study was funded by National Natural Science Foundation of China (No. 30771540) and National High-Technology Research Development Program of China (No. 2007AA10Z166).

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Authors and Affiliations

  1. College of Animal Science and Technology, China Agricultural University, Beijing, 100193, People’s Republic of China

    Guiying Wang, Lujun Kong, Peng Hu, Jinlian Fu & Aiguo Wang

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  1. Guiying Wang
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  2. Lujun Kong
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  3. Peng Hu
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  4. Jinlian Fu
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  5. Aiguo Wang
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Corresponding authors

Correspondence to Jinlian Fu or Aiguo Wang.

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Guiying Wang and Lujun Kong contributed equally to this work.

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Wang, G., Kong, L., Hu, P. et al. Effect of polymorphism in the peroxisome proliferator-activated receptor gamma gene on litter size of pigs. Mol Biol Rep 38, 1807–1812 (2011). https://doi.org/10.1007/s11033-010-0296-7

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  • DOI: https://doi.org/10.1007/s11033-010-0296-7

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