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Allelic variability of estrogen receptor (ESR) gene and its effect on litter traits of Doom pigs

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Abstract

The present study was conducted to identify the polymorphism of estrogen receptor (ESR) gene and its biological association with litter traits (litter size at birth, litter size at weaning, litter weight at birth and litter weight at weaning) of Doom pigs native to Assam. A total of 50 adult pigs (12 males and 38 females) chosen randomly from three different herds (Herd I, Herd II, and Herd III) were utilized in the present study. Detection of polymorphism of ESR gene was done by means of PCR-RFLP method. The amplified PCR product was digested with Pvu II restriction enzyme. PCR-RFLP analysis of ESR gene revealed polymorphic banding pattern. Two genotypes viz. AA and AB were identified. AA genotype yielded one fragment (120 bp) and AB genotype yielded three fragments (120, 65, and 55 bp). BB genotype was not found in the population under study. The frequencies of A and B alleles were found to be 0.650 and 0.350, respectively, and the genotypic frequencies of ESR gene were found to be 0.300 and 0.700 for AA and AB genotypes, respectively. There was no significant (P > 0.05) effect of ESR genotype on litter traits and the population under study was not in Hardy-Weinberg Equilibrium for ESR gene. Clustal W Multiple alignment of partial sequence of ESR gene revealed single nucleotide changes at 33, 65, 70, 83, and 92nd nucleotide positions. The presence of Pvu II polymorphism and identification of single nucleotide variation of ESR gene opens interesting prospects for improvement of litter traits in Doom pig through selective breeding program, especially based on marker-assisted selection.

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Abbreviations

ESR:

estrogen receptor

DNA:

deoxyribonucleic Acid

EDTA:

ethylenediamine tetraacetic acid

PCR:

polymerase chain reaction

RFLP:

restriction fragment length polymorphism

SDS:

sodium dodecyl sulfate

OD:

optical density

DMRT:

Duncan’s multiple range test

Bp:

base pair

SNP:

single nucleotide polymorphism

LSM:

least squares mean

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Acknowledgements

The authors thankfully acknowledge Assam Agricultural University (AAU), Khanpara, Assam, India, for providing the necessary facilities to conduct the research. The authors also acknowledge Molbiogen (Apical Scientific) for their significant contribution in the experiment.

Funding

Assam Agricultural University, Khanapara, India-781022

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Authors and Affiliations

  1. Department of Animal Genetics and Breeding, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, Assam, 781022, India

    Mokidur Rahman

  2. Department of Animal Genetics and Breeding, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, Assam, 781022, India

    Arundhati Phookan, Galib Uz Zaman, Arpana Das & Lopa M. Sarma

  3. Goat Research Station, Assam Agricultural University, Burnihat, Assam, 782402, India

    Farzin Akhtar

  4. Department of Veterinary Biochemistry, College of Veterinary Science, Assam Agricultural University, Khanapara, Guwahati, Assam, 781022, India

    Shantanu Tamuly

  5. Krishi Vigyan Kendra, Dudhnoi, Assam, 783124, India

    Hitu Choudhury

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  1. Mokidur Rahman
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  2. Arundhati Phookan
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  3. Galib Uz Zaman
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  4. Arpana Das
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  5. Farzin Akhtar
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  6. Shantanu Tamuly
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  7. Hitu Choudhury
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  8. Lopa M. Sarma
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Contributions

MR: Conducted the experiment, AP: Design and guided the experiment, GUZ and AD: Statistical Analysis, FA and ST: Molecular analysis of data, HC and LMS: Helps in data collection, recording and manuscript writing.

Corresponding author

Correspondence to Mokidur Rahman.

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Ethics approval and consent to participate

The Research was conducted after getting approval from Institutional Animal Ethics Committee (IEAC), AAU, Khanapara, India-781022 (Approval No. 770/ac/CPCSEA/F.V.Sc./AAU/IEAC/17-18/477).

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The authors declare that there is no conflict of interest for this study.

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Rahman, M., Phookan, A., Zaman, G.U. et al. Allelic variability of estrogen receptor (ESR) gene and its effect on litter traits of Doom pigs. Trop Anim Health Prod 53, 316 (2021). https://doi.org/10.1007/s11250-021-02756-6

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