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Behavioral effects of citrus paradisi in rats

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Abstract

Anxiety and depression adversely affect behavior though these conditions may be handled through environmental and dietary changes. Diet rich in flavonoids and vitamins may support reducing anxiety and depression. Depression is most common but serious illness making life miserable; however the usage of dietary and herbal complements to treat anxiety and depression had been grown by the time. The purpose of this investigation was to characterize the behavioral properties of Citrus paradisi in rats at diverse doses i.e. 0.1, 0.3 and 0.5 ml/kg. Anxiolytic and antidepressant actions were particularly measured twice in 15 days through elevated plus maze, open field and forced swimming tests. C. paradisi, revealed increase in the locomotor activity and the exploratory skills of the animals, as assessed in the open-field. Indeed C. paradisi had a strong anxiolytic effect in elevated plus-maze, as assessed by an augmented number of entries and the proportion of time spent in the open arms. Moreover there was decline in duration of immobility and rise in duration of climbing during forced swimming test. At the tested doses these results suggest that C. paradisi have the potential to exert a range of CNS-mediated biological activities and thus encourage more investigations in this field.

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References

  • Alves R, de Carvalho JGB, Benedito MCA (2005) High and low rearing subgroups of rats selected in the open field differ in the activity of K+ − stimulated p-nitrophenylphosphatase in the hippocampus. Brain Res 1058(1–2):178–182.

  • Arieti S, Bemporad J (1980) The psychological organization of depression. Am J Psychiatr 137:1360–1365

    Article  CAS  PubMed  Google Scholar 

  • Buddenberg TE, Komorowski M, Ruoccob LA, Silva MA (2009) Attenuating effects of testosterone on depressive-like behavior in the forced swim test in healthy male rats. Brain Res Bull 79:182–186. doi:10.1016/j.brainresbull.2009.02.008

    Article  CAS  PubMed  Google Scholar 

  • Budzynska B, Boguszewska-Czubara A, Kruk-Slomka M, Skalicka- Woziniak K, Michalak A, Musik I, Biala G, Glowniak K (2013) Effects of imperatorin on nicotine induced anxiety and memory related responses and oxidative stress in mice. Physiol Behav 122:46–55.

  • Carbajal D, Ravelo Y, Molina V, Mas R, Arruzazabala ML (2009) D-004 a lipid extract from royal palm fruit, exhibits antidepressant effects in the forced swim test and the tail suspension test in mice. Pharmacol Biochem Behav 92:465–468

    Article  CAS  PubMed  Google Scholar 

  • Council NR (1996) Guide for the care and use of laboratory animals. National Academy Press, Washington, pp. 1–7

    Google Scholar 

  • Czech Donald A (2002) A simple integrated circuit device for measuring distances traveled and determining speed in open-field environments. Pharmacol Biochem Behav 72(1–2):73–75

    Article  CAS  PubMed  Google Scholar 

  • Evans DL, Charney DS, Lewis L, Golden JM, Krishnan KRR, Nemeroff CB (2005) Mood disorders in the medically ill: scientific review and recommendations. Biol Psychiatry 58:175–189

    Article  PubMed  Google Scholar 

  • Griebel G, Perrault G, Tan S, Schoemaker H, Sanger DJ (1999) Pharmacological studies on synthetic flavonoids: comparison with diazepam. Neuropharmacology 38:965–977

    Article  CAS  PubMed  Google Scholar 

  • Gupta V, Bansal P, Kumar P, Kaur G (2010a) Pharmacopoeial standards and pharmacognostical studies of leaves of citrus paradisi Var. Foster. Res J Pharmacog Phytochem 2:140–143

    Google Scholar 

  • Gupta V, Bansal P, Kumar P, Shri R (2010b) Anxiolytic and antidepressant activities of different extracts from citrus paradisi var. Duncan Asian. J Pharm Clin Res 3(2):98–100

    Google Scholar 

  • Gupta V, Bansal P, Niazi J, Kaur G (2010c) Anti-anxiety activity of citrus paradisi var. star ruby extracts. Int J PharmTech Res 2(3):1655–1657

    Google Scholar 

  • Gupta V, Kohli K, Ghaiye P, Bansal P, Lather A (2011) Pharmacological potentials of citrus paradisi – an overview. Int J Phytotherapy Res 1(1):8–17

    Google Scholar 

  • Gureje O, Von Korff M, Simon GE, Gater R (1998) Persistent pain and wellbeing: a World Health Organization study in primary care. JAMA 280:147–151

    Article  CAS  PubMed  Google Scholar 

  • Herskin MS, Jensen KH (2002) Effects of open field testing and associated handling v. handling alone on the adrenocortical reactivity of piglets around weaning. Anim Sci 74:485–491

    Google Scholar 

  • Hogg S (1996) A review of the validity and variability of the elevated plus-maze as an animal model of anxiety. Pharmacol Biochem Behav 54(1):21–30

    Article  CAS  PubMed  Google Scholar 

  • Kennett GA, Dourish CT, Curzon G (1987) Antidepressant-like action of 5-HT1A agonists and conventional antidepressants in an animal model of depression. Eur J Pharmacol 134(3):265–274

    Article  CAS  PubMed  Google Scholar 

  • Komiya M, Takeuchi T, Harada E (2006) Lemon oil vapor causes an anti-stress effect via modulating the 5-HT and DA activities in mice. Behav Brain Res 172:240–249

    Article  CAS  PubMed  Google Scholar 

  • Kumar S, Sharma A (2005) Anti-anxiety activity studies of various extracts of turnera aphrodisiaca ward. J Herb Pharmacother 5:13–21

    Article  PubMed  Google Scholar 

  • Lather A, Gupta V, Chaudhary AK, Singh R, Bansal P, Ghaiye P, Bansal R (2012) In vitro evaluation of antimicrobial activity of Kutajghan Vati - an Ayurvedic Formulation Pak. J Pharm Sci 25(3):693–696

    CAS  Google Scholar 

  • Mansouri MT, SoltaniM NB, Farbood Y, Mashak A, Sarkaki A (2014) A possible mechanism for the anxiolytic-like effect of gallic acid in the rat elevated plus maze. Pharmacol Biochem Behav 117:40–46

    Article  CAS  PubMed  Google Scholar 

  • More S, Sathe S, Sonawane A, Jadhav A, Kadam V (2013) Citrus paradisi: an overview, Indo American. J Pharm Res 3(10):8070–8077

    Google Scholar 

  • Novas ML, Wolfman C, Jorge H, De Robertis E (1988) Proconvulsantand ‘anxiogenic’ effects of n-butyl β carboline-3-carboxylate, an endogenous benzodiazepine binding inhibitor from brain. Pharmacol Biochem Behav 30:331–336

    Article  CAS  PubMed  Google Scholar 

  • Palazzolo E, Laudicina VA, Germanà MA (2013) Current and potential use of citrus essential oils. Curr Org Chem 17:3042–3049

    Article  CAS  Google Scholar 

  • Pellow S, Sandra FE (1986) Anxiolytic and anxiogenic drug effects on exploratory activity in an elevated plus-maze:anovel test of anxiety in the rat. Pharmacol Biochem Behav 24(3):525–529

    Article  CAS  PubMed  Google Scholar 

  • Petit-Demouliere B, Chenu F, Bourin M (2005) Forced swimming test in mice: a review of antidepressant activity. Psychopharmacology 177:245–255

    Article  CAS  PubMed  Google Scholar 

  • Pine DS, Wasserman GA, Workman SB (1999) Memory and anxiety in pre-pubertal boys at risk for delinquency. J Am Acad Child Adolesc Psychiatry 38:1024–1031

    Article  CAS  PubMed  Google Scholar 

  • Porsolt RD, Anton G, Blavet N, Jalfre M (1987) Behavioral despair in rats: a new model sensitive to antidepressant treatments. Eur J Pharmacol 47(4):379–391

    Article  Google Scholar 

  • Porsolt RD, Le Pichon M, Jalfre M (1977) Depression: a new animal model sensitive to antidepressant treatments. Nature 266:730–732

    Article  CAS  PubMed  Google Scholar 

  • Prut L, Belzung (2003) The open field as a paradigm to measure the effects of drugs on anxiety-like behaviors: a review. Eur J Pharmacol 463(1–3):3–33

  • Reynolds EH (2003) Brain and mind: a challenge for WHO. Lancet 361:1924–1925

    Article  CAS  PubMed  Google Scholar 

  • Sadock BJ, Sadock VA, Sadock’s K (2003) Synopsis of psychiatry— behavioral sciences/clinical psychiatry, 9th edn. Lippincott Williams and Wilkins, Philadelphia

    Google Scholar 

  • Soderpalm B, Svensson L, Hulthe P, Johannessen K, Engel JA (1991) Evidence for a role for dopamine in the diazepam locomotor stimulating effect. Psychopharmacology 104:97–102

    Article  CAS  PubMed  Google Scholar 

  • Spinella M (2001) Herbal medicines and epilepsy: the potential for benefit and adverse effects. Epilepsy Behav 2:524–532

    Article  PubMed  Google Scholar 

  • Taiwo Adefunmilayo E, Leite Franco B, Lucena Greice M, Barros M, Silveira D, Silva Monica V, Ferreira Vania M (2012) Anxiolytic and antidepressant-like effects of Melissa officinalis (lemon balm) extract in rats: influence of administration and gender. Indian J Pharmacol 44:189–192

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Thase ME, Howland RH (1995) Biological processes in depression: an update and integration. In: Beckham EE, Leber WR (eds) Handbook of depression, 2nd edn. Guilford, New York, pp. 213–279

    Google Scholar 

  • The World Health Report (2001) Mental health: new understanding new hope. WHO, Geneva

    Google Scholar 

  • Wilner P (1984) The validity of animal models of depression. Psychopharmacology 83:1–16

    Article  Google Scholar 

  • Wolfman C, Viola H, Paladini A, Dajas F, Medina JH (1994) Possible anxiolytic effects of chrysin, a central benzodiazepine receptor ligand isolated from passiflora coerulea. Pharmacol Biochem Behav 47:1–4

    Article  CAS  PubMed  Google Scholar 

  • Yang S-A, S-Kyung J, E-Jung L, C-Hyun S, In-Seon L (2010) Comparative study of the chemical composition and antioxidant activity of six essential oils and their components. Nat Prod Res 24:140–151

    Article  CAS  PubMed  Google Scholar 

  • Zhang ZJ (2004) Therapeutic effects of herbal extracts and constituents in animal models of psychiatric disorders. Life Sci 75:1659–1699

    Article  CAS  PubMed  Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Pharmacology, Faculty of Pharmacy, University of Karachi, Karachi, 75270, Pakistan

    Neelam Mallick & Rafeeq Alam Khan

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  1. Neelam Mallick
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  2. Rafeeq Alam Khan
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Correspondence to Rafeeq Alam Khan.

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Mallick, N., Khan, R.A. Behavioral effects of citrus paradisi in rats. Metab Brain Dis 31, 329–335 (2016). https://doi.org/10.1007/s11011-015-9754-1

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