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Repositing honey incorporated electrospun nanofiber membranes to provide anti-oxidant, anti-bacterial and anti-inflammatory microenvironment for wound regeneration

  • Tissue Engineering Constructs and Cell Substrates
  • Original Research
  • Published:
Journal of Materials Science: Materials in Medicine Aims and scope Submit manuscript

Abstract

Topical application of honey for tissue regeneration, has recently regained attention in clinical practice with controlled studies affirming its efficacy and indicating its role in regeneration over repair. Parallely, to overcome difficulties of applying raw honey, several product development studies like nanofibrous matrices have been reported. However, one approach concentrated on achieving highest possible honey loading in the nanofiber membranes while other studies have found that only specific honey dilutions result in differential cellular responses on wound healing and re-epithelization. From these results, it can be suggested that high honey loading provides optimum external microenvironment, low-loaded membranes could provide a more conducive internal microenvironment for tissue regeneration. With this hypothesis, this paper sought to evaluate ability of low-honey loaded nanofibers to modulate the anti-oxidant, anti-biofilm and anti-inflammatory properties which are important to be maintained in wound micro-environment. A loading-dependent reduction of biofilm formation and anti-oxidant activity was noted in different concentration ranges investigated. After scratch assay, a certain honey loading (0.5%) afforded the maximum re-epithelization. Since there is lack of methods to determine anti-inflammatory properties of nanofiber membranes during epithelial healing process, we performed anti-inflammatory assessment of nano-fibers by evaluating the expressions of pro-inflammatory markers-Cycloxygenase-2 (COX-2) and Interleukin-6 (IL-6) and to confirm the optimized concentration. Considering the role of COX-2 and IL-6, the novel methodology used in this study can also be developed as an assay for anti-inflammatory matrices for wound healing.

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Abbreviations

COX-2:

Cycloxygenase-2

IL6:

Interleukin-6

PNL:

Polymorphonuclear luekocyte

MMPs:

Metalloproteinases

PDGF:

Platelet derived growth factor

VEGF:

Vascular endothelial growth factor

TGF-β:

Transforming growth factor

ECM:

Extra cellular matrix

MTT:

3-[4,5-dimethylthiazol-2-yl]-diphenyltetrazolium bromide

BrdU:

Bromodeoxyuridine

FBS:

Fetal bovine serum

PVA:

Poly vinyl alcohol

SEM:

Scanning Electron Microscopy

MTT:

3-[4,5-dimethylthiazol-2-yl]-diphenyltetrazolium bromide

BrdU:

Bromodeoxyuridine

FBS:

fetal bovine serum

PVA:

Poly vinyl alcohol

SEM:

Scanning Electron Microscopy

DMEM:

Dulbecco’s modified Eagle’s medium

DIC:

Differential interference contrast

PH:

PVA-honey

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Acknowledgements

AB would like to acknowledge SERB, Govt. of India financial assistance through Fast Track project numbers SB/FTP/ETA/265-2012 and DST INSPIRE faculty award to PD via IFA2012-LSBM-48. Assistance from TEQIP Phase II to IIEST Shibpur for procurement of Inverted Florescence microscope and TEQIP COE for AFM is also acknowledged.

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  1. Centre for Healthcare Science and Technology, Indian Institute of Engineering Science and Technology Shibpur, P.O. Botanic Garden, Howrah, West Bengal, 711103, India

    Ripon Sarkar, Aritri Ghosh, Ananya Barui & Pallab Datta

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Correspondence to Pallab Datta.

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Sarkar, R., Ghosh, A., Barui, A. et al. Repositing honey incorporated electrospun nanofiber membranes to provide anti-oxidant, anti-bacterial and anti-inflammatory microenvironment for wound regeneration. J Mater Sci: Mater Med 29, 31 (2018). https://doi.org/10.1007/s10856-018-6038-4

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