Promotion of adhesion and proliferation of endothelial progenitor cells on decellularized valves by covalent incorporation of RGD peptide and VEGF

  • Jianliang ZhouEmail author
  • Jingli Ding
  • Bin’en Nie
  • Shidong Hu
  • Zhigang Zhu
  • Jia Chen
  • Jianjun Xu
  • Jiawei Shi
  • Nianguo DongEmail author
Engineering and Nano-engineering Approaches for Medical Devices Original Research
Part of the following topical collections:
  1. Engineering and Nano-engineering Approaches for Medical Devices


Tissue engineered heart valve is a promising alternative to current heart valve surgery, for its capability of growth, repair, and remodeling. However, extensive development is needed to ensure tissue compatibility, durability and antithrombotic potential. This study aims to investigate the biological effects of multi-signal composite material of polyethyl glycol-cross-linked decellularized valve on adhesion and proliferation of endothelial progenitor cells. Group A to E was decellularized valve leaflets, composite material of polyethyl glycol-cross-linked decellularized valves leaflets, vascular endothelial growth factor-composite materials, Arg-Gly-Asp peptide-composite materials and multi-signal modified materials of polyethyl glycol-cross-linked decellularized valve leaflets, respectively. The endothelial progenitor cells were seeded for each group, cell adhesion and proliferation were detected and neo-endothelium antithrombotic function of the multi-signal composite materials was evaluated. At 2, 4, and 8 h after the seeding, the cell numbers and 3H-TdR incorporation in group D were the highest. At 2, 4, and 8 days after the seeding, the cell numbers and 3H-TdR incorporation were significantly higher in groups C, D, and E compared with groups A and B (P < 0.05) and cell numbers and the expression of t-PA and eons in the neo-endothelium were quite similar to those in the human umbilical vein endothelial cells at 2, 4, and 8 days after the seeding. The Arg-Gly-Asp- peptides (a sequential peptide composed of arginine (Arg), glycine (Gly) and aspartic acid (Asp)) and VEGF-conjugated onto the composite material of PEG-crosslinked decellularized valve leaflets synergistically promoted the adhesion and proliferation of endothelial progenitor cells on the composite material, which may help in tissue engineering of heart valves.


Vascular Endothelial Growth Factor Endothelial Progenitor Cell Valve Leaflet Acryloyl Biological Valve 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



This study was funded by a grant from the National High-tech Research and Development Program (863 Program) of China (No. 2014AA020539), the National Natural Science Foundation of China (No. 81260047, No. 81270297, and No. 31330029), and the Youth Science Foundation of Jiangxi Province, China (20122BAB215016).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Jianliang Zhou
    • 1
    Email author
  • Jingli Ding
    • 2
  • Bin’en Nie
    • 3
  • Shidong Hu
    • 1
  • Zhigang Zhu
    • 1
  • Jia Chen
    • 1
  • Jianjun Xu
    • 1
  • Jiawei Shi
    • 4
  • Nianguo Dong
    • 4
    Email author
  1. 1.Department of Cardiothoracic Surgerythe Second Affiliated Hospital of Nanchang UniversityNanchangChina
  2. 2.Department of Gastroenterologythe Second Affiliated Hospital of Nanchang UniversityNanchangChina
  3. 3.Shanghai Key Laboratory of Orthopedic ImplantsDepartment of Orthopedic Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of MedicineShanghaiChina
  4. 4.Department of Cardiovascular Surgerythe Union Hospital Affiliated to Tongji Medical College of Huazhong University of Science and TechnologyWuhanChina

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