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Multifarious plant growth promoting characteristics of chickpea rhizosphere associated Bacilli help to suppress soil-borne pathogens

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Abstract

Wilt and root rot are the major constraints in chickpea production and very difficult to manage through agrochemicals. Hence, for an ecofriendly and biological management, 240 strains of Bacillus and Bacillus derived genera were isolated from chickpea rhizosphere, further narrowed down to 14 strains on the basis of in vitro production of indole acetic acid, siderophore, phosphate solubilization, hydrolytic enzymes and were evaluated for antagonism against chickpea pathogens (Fusarium oxysporum f. sp. ciceri race 1, F. solani and Macrophomina phaseolina). The strains were identified on the basis of physiological characters and 16S RNA gene sequencing. The genotypic comparisons of strains were determined by BOX-polymerase chain reaction profiles and amplified rDNA restriction analysis. These isolates were evaluated in greenhouse assay in which B. subtilis (B-CM191, B-CV235, B-CL-122) proved to be effective in reducing wilt incidence and significant enhancement in growth (root and shoot length) and dry matter of chickpea plants. PCR amplification of bacillomycin (bmyB) and β-glucanase genes suggests that amplified genes from the Bacillus could have a role to further define the diversity, ecology, and biocontrol activities in the suppression of soil-borne pathogens.

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Acknowledgments

This work has been carried out under the network project ‘Application of Microorganisms in Agricultural and Allied Sectors’ and financially supported by Indian Council of Agricultural Research (ICAR), New Delhi, India.

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Authors and Affiliations

  1. National Bureau of Agriculturally Important Microorganisms, Mau, 275101, Uttar Pradesh, India

    Rajesh Kumar Singh, D. Praveen Kumar, Pratiksha Singh, Manoj Kumar Solanki, Supriya Srivastava, Prem Lal Kashyap, Sudheer Kumar, Alok K. Srivastava & Dilip K. Arora

  2. Department of Biological Science, Rani Durgavati University, Jabalpur, 482001, Madhya Pradesh, India

    Pradeep K. Singhal

Authors
  1. Rajesh Kumar Singh
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  2. D. Praveen Kumar
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  3. Pratiksha Singh
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  4. Manoj Kumar Solanki
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  5. Supriya Srivastava
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  6. Prem Lal Kashyap
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  7. Sudheer Kumar
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  8. Alok K. Srivastava
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  9. Pradeep K. Singhal
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  10. Dilip K. Arora
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Correspondence to Sudheer Kumar.

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Singh, R.K., Kumar, D.P., Singh, P. et al. Multifarious plant growth promoting characteristics of chickpea rhizosphere associated Bacilli help to suppress soil-borne pathogens. Plant Growth Regul 73, 91–101 (2014). https://doi.org/10.1007/s10725-013-9870-z

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  • DOI: https://doi.org/10.1007/s10725-013-9870-z

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