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Plant Growth Regulation

, Volume 73, Issue 1, pp 91–101 | Cite as

Multifarious plant growth promoting characteristics of chickpea rhizosphere associated Bacilli help to suppress soil-borne pathogens

  • Rajesh Kumar Singh
  • D. Praveen Kumar
  • Pratiksha Singh
  • Manoj Kumar Solanki
  • Supriya Srivastava
  • Prem Lal Kashyap
  • Sudheer KumarEmail author
  • Alok K. Srivastava
  • Pradeep K. Singhal
  • Dilip K. Arora
Original paper

Abstract

Wilt and root rot are the major constraints in chickpea production and very difficult to manage through agrochemicals. Hence, for an ecofriendly and biological management, 240 strains of Bacillus and Bacillus derived genera were isolated from chickpea rhizosphere, further narrowed down to 14 strains on the basis of in vitro production of indole acetic acid, siderophore, phosphate solubilization, hydrolytic enzymes and were evaluated for antagonism against chickpea pathogens (Fusarium oxysporum f. sp. ciceri race 1, F. solani and Macrophomina phaseolina). The strains were identified on the basis of physiological characters and 16S RNA gene sequencing. The genotypic comparisons of strains were determined by BOX-polymerase chain reaction profiles and amplified rDNA restriction analysis. These isolates were evaluated in greenhouse assay in which B. subtilis (B-CM191, B-CV235, B-CL-122) proved to be effective in reducing wilt incidence and significant enhancement in growth (root and shoot length) and dry matter of chickpea plants. PCR amplification of bacillomycin (bmyB) and β-glucanase genes suggests that amplified genes from the Bacillus could have a role to further define the diversity, ecology, and biocontrol activities in the suppression of soil-borne pathogens.

Keywords

Bacillus Chickpea Fusarium wilt Genetic fingerprinting Plant growth promoting rhizobacteria 

Notes

Acknowledgments

This work has been carried out under the network project ‘Application of Microorganisms in Agricultural and Allied Sectors’ and financially supported by Indian Council of Agricultural Research (ICAR), New Delhi, India.

Supplementary material

10725_2013_9870_MOESM1_ESM.docx (17 kb)
Supplementary material 1 (DOCX 16 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Rajesh Kumar Singh
    • 1
  • D. Praveen Kumar
    • 1
  • Pratiksha Singh
    • 1
  • Manoj Kumar Solanki
    • 1
  • Supriya Srivastava
    • 1
  • Prem Lal Kashyap
    • 1
  • Sudheer Kumar
    • 1
    Email author
  • Alok K. Srivastava
    • 1
  • Pradeep K. Singhal
    • 2
  • Dilip K. Arora
    • 1
  1. 1.National Bureau of Agriculturally Important MicroorganismsMauIndia
  2. 2.Department of Biological ScienceRani Durgavati UniversityJabalpurIndia

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