Abstract
Salicylic acid (SA) is one of the important signal molecules modulating plant responses to environmental stress. In this study, the effects of exogenous SA on leaf rolling, one of drought avoidance mechanisms, and antioxidant system were investigated in Ctenanthe setosa during long term drought stress. The plants were subjected to 38-day drought period and they were treated with or without SA (10−6 M) on the 25th, 27th and 29th days of the period. Leaf samples were harvested on the 30th, 34th and 38th days. Some antioxidant enzyme activities (superoxide dismutase, catalase, ascorbate peroxidase, dehydroascorbate reductase, monodehydroascorbate reductase, glutathione reductase), reactive oxygen species (hydrogen peroxide and superoxide) and lipid peroxidation were determined during the drought period. Treatment with SA prevented water loss and delayed leaf rolling in comparison with control leaves. Exogenous SA induced all antioxidant enzyme activities more than control leaves during the drought. Ascorbate and glutathione, α-tocopherol, carotenoid and endogenous SA level were induced by the SA treatment. Levels of reactive oxygen species were higher in SA treated plants than control ones on the 34th day. Their levels on the 38th day, however, fastly decreased in SA treated plants. SA treatment prevented lipid peroxidation while the peroxidation increased in control plants. The results showed that exogenous SA can alleviate the damaging effect of long term drought stress by decreasing water loss and inducing the antioxidant system in the plant having leaf rolling, alternative protection mechanism to drought.
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Abbreviations
- APX:
-
Ascorbate peroxidase
- ASC:
-
Ascorbic acid
- CAT:
-
Catalase
- DHA:
-
Dehydroascorbate
- DHAR:
-
Dehydroascorbate reductase
- gs :
-
Stomatal conductance
- GR:
-
Glutathione reductase
- GSH:
-
Reduced glutathione
- H2O2 :
-
Hydrogen peroxide
- MDA:
-
Malondialdehyde
- MDHAR:
-
Monodehydroascorbate reductase
- NBT:
-
Nitro blue tetrazolium
- O •−2 :
-
Superoxide anion radical
- ROS:
-
Reactive oxygen species
- RWC:
-
Relative water content
- SOD:
-
Superoxide dismutase
- Ψleaf :
-
Leaf water potential
References
Aebi H (1983) Catalase. In: Bergmeyer H (ed) Methods of enzymatic analysis. Weinheim-Verlag Chemie, Weinheim, pp 273–286
Al-Hakimi AMA, Hamada AM (2001) Counteraction of salinity stress on wheat plants by grain soaking in ascorbic acid, thiamin or sodium salicylate. Biol Plant 44:253–261
Arfan M, Athar HR, Ashraf M (2007) Does exogenous application of salicylic acid through the rooting medium modulate growth and photosynthetic capacity in two differently adapted spring wheat cultivars under salt stress? J Plant Physiol 6(4):685–694
Beauchamp C, Fridovich I (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem 44:276–287
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
Castillo FJ (1996) Antioxidative protection in the inducible CAM plant Sedum album L. following the imposition of severe water stress and recovery. Oecologia 107:469–477
Chen Z, Silva H, Klessig DF (1993) Active oxygen species in the induction of plant systemic acquired resistance by salicylic acid. Science 262:1883–1886
Clarke JM (1986) Effect of leaf rolling on leaf water loss in Triticum spp. Can J Plant Sci 66:885–891
Dat JF, Foyer CH, Scott IM (1998) Changes in salicylic acid and antioxidants during induced thermotolerance in mustard seedlings. Plant Physiol 118:1455–1461
Dat JF, Lopez-Delgado H, Foyer CH, Scott IM (2000) Effects of salicylic acid on oxidative stress and thermotolerance in tobacco. J Plant Physiol 156:659–665
Dhindsa RS, Matowe W (1981) Drought tolerance in two mosses: correlated with enzymatic defence against lipid peroxidation. J Exp Bot 32:79–91
Farooq M, Basra SMA, Wahid A, Ahmad N, Saleem BA (2009) Improving the drought tolerance in rice (Oryza sativa L.) by exogenous application of salicylic acid. J Agron Crop Sci 195:237–246
Foyer CH, Halliwell B (1976) Presence of glutathione and glutathione reductase in chloroplast: a proposed role in ascorbic acid metabolism. Planta 133:21–25
Foyer CH, Noctor G (2003) Redox sensing and signalling associated with reactive oxygen in chloroplasts, peroxisomes and mitochondria. Physiol Plant 119:355–364
Foyer CH, Rowell J, Walker D (1983) Measurement of the ascorbate content of spinach leaf protoplasts and chloroplasts during illumination. Planta 157:239–244
Foyer CH, Descouvieres P, Kunert KJ (1994) Protection against oxygen radicals: an important mechanism studied in transgenic plants. Plant Cell Environ 17:507–523
Frahry G, Schopfer P (2001) NADH-stimulated, cyanide-resistant superoxide production in maize coleoptiles analyzed with a tetrazolium-based assay. Planta 212:175–183
Gonzales L, Gonzales-Vilar M (2001) Determination of relative water content. In: Reigosa MJ (ed) Handbook of plant ecophysiology techniques. Kluwer Academic Publishers, Dordrecht, pp 207–212
Griffith OW (1980) Determination of glutathione and glutathione disulfide using glutathione reductase and 2-vinylpyridine. Anal Biochem 106:207–221
Gruszka J, Kruk J (2007) RP-LC for determination of plastochromanol, tocotrienols and tocopherols in plant oils. Chromatographia 66:909–913
Hayat S, Hasan SA, Fariduddin Q, Ahmad A (2008) Growth of tomato (Lycopersicon esculentum) in response to salicylic acid under water stress. J Plant Interact 3(4):297–304
Hayat Q, Hayat S, Irfan M, Ahmad A (2010) Effect of exogenous salicylic acid under changing environment: a review. Environ Exp Bot 68(1):14–25
Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplast. I. Kinetics and stochiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189–198
Hossain MA, Asada K (1984) Purification of dehydroascorbate reductase from spinach and its characterization as a thiol enzyme. Plant Cell Physiol 25:85–92
Hossain MA, Nakano Y, Asada K (1984) Monodehydroascorbate reductase in spinach chloroplasts and its participation in regeneration of ascorbate for scavenging hydrogen peroxide. Plant Cell Physiol 25:385–395
Hsiao TC, O’Toole JC, Yambao EB, Turner NC (1984) Influence of osmotic adjustment on leaf rolling and tissue death in rice (Oryza sativa L.). Plant Physiol 75:338–341
Huang RH, Liu JH, Lu YM, Xia RX (2008) Effect of salicylic acid on the antioxidant system in the pulp of ‘Cara cara’ navel orange (Citrus sinensis L. Osbeck) at different storage temperatures. Postharvest Biol Technol 47:168–175
Janda T, Szalai G, Tari I, Paldi E (1999) Hydroponic treatment with salicylic acid decreases the effect of chilling injury in maize (Zea mays L.) plants. Planta 208:175–180
Janda T, Szalai G, Rios-Gonzales K, Veisz O, Paldi E (2003) Comparative study of frost tolerance and antioxidant activity in cereals. Plant Sci 164:301–306
Jaspars EMJ (1965) Pigmentation of tobacco crown gall tissues cultured in vitro in dependence of the composition of the medium. Physiol Plant 18:933–940
Kadioglu A, Terzi R (2007) A dehydration avoidance mechanism: leaf rolling. Bot Rev 73:290–302
Kadioglu A, Turgut R, Palavan-Ünsal N, Saruhan N (2002) Effect of polyamines on leaf rolling in Ctenanthe setosa. Isr J Plant Sci 50:19–23
Klessig DF, Durner J, Noad R, Navarre A, Wendehenne D, Kumar D, Zhou JM, Shah J, Zhang S, Kachroo P, Trifa Y, Pontier D, Lam E, Slva H (2000) Nitric oxide and salicylic acid signalling in plant defense. Proc Natl Acad Sci USA 97:8849–8855
Landberg T, Greger M (2002) Differences in oxidative stress in heavy metal resistant and sensitive clones of Salix viminalis. J Plant Physiol 159:69–75
Lang YZ, Zhang ZJ, Gu XY, Yang JC, Zhu QS (2004) A physiological and ecological effect of crimpy leaf character in rice (Oryza sativa L.) II. Photosynthetic character, dry mass production and yield forming. Acta Agron Sin 30:883–887
Li L, Staden JV (1998) Effect of plant growth regulators on the antioxidant system in callus of two maize cultivars subjected to water stress. Plant Growth Regul 24:55–66
Minibaeva FV, Gordon LK, Kolesnikov OP (2001) Role of extracellular peroxidase in the superoxide production by wheat root cells. Protoplasma 217:125–128
Mittler R (2002) Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci 7:405–410
Möller IM (2001) Plant mitochondria and oxidative stress: electron transport, NADPH turnover, and metabolism of reactive oxygen species. Annu Rev Plant Physiol Plant Mol Biol 52:561–591
Munné-Bosch S, Alegre L (2002) The function of tocopherols and tocotrienols in plants. Crit Rev Plant Sci 21:31–57
Munné-Bosch S, Penuelas J (2003) Photo and antioxidative protection, and a role for salicylic acid during drought and recovery in field grown Phillyrea angustifolia plants. Planta 217:758–766
Nakano Y, Asada K (1987) Purification of ascorbate peroxidase in spinach-chloroplasts-its inactivation in ascorbate-depleted medium and reactivation by monodehydroascorbate radical. Plant Cell Physiol 28:131–140
Nar H, Saglam A, Terzi R, Varkonyi Z, Kadioglu A (2009) Leaf rolling and photosystem II efficiency in Ctenanthe setosa exposed to drought stress. Photosynthetica 47:429–436
Noctor G, Foyer CH (1998) Ascorbate and glutathione: keeping active oxygen under control. Ann Rev Plant Physiol Plant Mol Biol 49:249–279
Popova LP, Maslenkova LT, Yordanova RY, Ivanova AP, Krantev AP, Szalai G, Janda T (2009) Exogenous treatment with salicylic acid attenuates cadmium toxicity in pea seedlings. Plant Physiol Biochem 47:224–231
Premachandra GS, Saneoka H, Fujita K, Ogata S (1993) Water stress and potassium fertilization in field grown maize (Zea mays L.): effects of leaf water relations and leaf rolling. J Agron Crop Sci 170:195–201
Rao MV, Paliyath G, Ormrod P, Murr DP, Watkins CB (1997) Influence of salicylic acid on H2O2 production, oxidative stress, and H2O2-metabolizing enzymes. Plant Physiol 115:137–149
Raskin I, Turner-Ivan M, Melander-Wayne R (1989) Regulation of heat production in the inflorescences of an Arum lily by endogenous salicylic acid. Proc Natl Acad Sci USA 86:2214–2218
Senaratna T, Touvhell D, Bunn E, Dixon K (2000) Acetyl salicylic acid (aspirin) and salicylic acid induce multiple stress tolerance in bean and tomato plant. Plant Growth Regul 30:157–161
Shakirova FM (2007) Role of hormonal system in the manifestation of growth promoting and antistress action of salicylic acid. In: Hayat S, Ahmad A (eds) Salicylic acid: a plant hormone. Springer, Dordrecht, pp 69–89
Shakirova FM, Sakhabutdinova AR, Bezrukova MV, Fatkhutdinova RA, Fatkhutdinova DR (2003) Changes in the hormonal status of wheat seedlings induced by salicylic acid and salinity. Plant Sci 164:317–322
Smirnoff N (1993) The role of active oxygen in response of plants to water deficit and desiccation. New Phytol 125:27–58
Talieva MN, Kondrat’eva VV (2002) Influence of exogenous salicylic acid on the level of phytohormones in tissues of Phlox paniculata and Phlox setacea leaves with special reference to resistance against the powdery mildew causative agent Erysiphe cichoracearum DC. f. phlogis Jacz. Biol Bull 29:551–554
Tari I, Csiszar J, Szalai G, Horvath F, Pecsvaradi A, Kiss G, Szepesi A, Szabo M, Erdei L (2002) Acclimation of tomato plants to salinity stress after a salicylic acid pre-treatment. Proceedings of the seventh Hungarian congress plant physiology. Acta Biol Szeged 46:55–56
Turgut R, Kadioglu A (1998) The effect of drought, temperature and irradiation on leaf rolling in Ctenanthe setosa. Biol Plant 41:629–663
Velikova V, Yordanov I, Edreva A (2000) Oxidative stress and some antioxidant systems in acid rain-treated bean plants. Protective role of exogenous polyamines. Plant Sci 151:59–66
Yalpani N, Enyedi AJ, Leon J, Raskin I (1994) Ultraviolet-light and ozone stimulate accumulation of salicylic acid, pathogenesis-related proteins and virus resistance in tobacco. Planta 193:372–376
Zhou SZ, Guo K, Elbaz AA, Yang ZM (2009) Salicylic acid alleviates mercury toxicity by preventing oxidative stress in roots of Medicago sativa. Environ Exp Bot 65:27–34
Acknowledgments
This work was supported by The Scientific and Technological Research Council of Turkey (TUBITAK, TBAG 108T298). The authors are grateful to Prof Dr Sevim Köse (Faculty of Marine Science, Karadeniz Technical University) and Serkan Koral (Faculty of Fisheries, Rize University) for determination of salicylic acid and α-tocopherol by HPLC.
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Kadioglu, A., Saruhan, N., Sağlam, A. et al. Exogenous salicylic acid alleviates effects of long term drought stress and delays leaf rolling by inducing antioxidant system. Plant Growth Regul 64, 27–37 (2011). https://doi.org/10.1007/s10725-010-9532-3
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DOI: https://doi.org/10.1007/s10725-010-9532-3