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Genetic Resources and Crop Evolution

, Volume 65, Issue 6, pp 1625–1635 | Cite as

Chromosomal distributions of oligo-Am1 and (TTG)6 trinucleotide and their utilization in genome association analysis of sixteen Avena species

  • Xiaomei Luo
  • Nick A. Tinker
  • Yonghong Zhou
  • Juncheng Liu
  • Wenlin Wan
  • Liang Chen
Research Article

Abstract

Fluorescence in situ hybridization was used to investigate the physical location of oligo-Am1 and (TTG)6 trinucleotide repeats in the metaphase chromosomes of seven diploid species (AA or CC genomes), seven tetraploid species (AABB or AACC genomes), and two hexaploid species (AACCDD genomes) belonging to the genus Avena. The oligo-Am1 probe produced signals that were particularly enriched on almost whole C-genome chromosomes, whereas the (TTG)6 probe was located in the pericentromeric (M), and, occasionally, their telomeric (T) chromosome regions, but showed low matching to C genome. All the species possessed (TTG)6 loci in M regions, and the CC, AABB, and AACCDD species also possessed (TTG)6 loci in T regions. The (TTG)6 signal number is constant in both the AA and CC species but slightly differs in signal intensity, whereas the (TTG)6 signal pattern shows wide diversity in the AABB, AACC and AACCDD species. The probe hybridization results provide key information that can be used in the physical assignment of genome sequences to chromosomes.

Keywords

Avena Chromosome markers FISH Signal distribution (TTG)6 

Abbreviations

DAPI

4,6-Diamidino-2-phenylindole

FAM

6-Carboxyfluorescein

TAMRM

6-Carboxytetramethylrhodamine

FISH

Fluorescence in situ hybridization

GISH

Genomic in situ hybridization

GBS

Genotyping-by-sequencing

M

Pericentromic

T

Telomeric

Notes

Acknowledgements

This study was supported by the Natural Science Foundation of China (31500993). The authors greatly appreciate the American National Plant Germplasm System (Pullman, WA, USA) and Plant Gene Resources of Canada (Saskatoon, SK, Canada) for providing the material for the investigation.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.College of ForestrySichuan Agricultural UniversityChengdu CityChina
  2. 2.Ottawa Research and Development CentreAgriculture and Agri-Food CanadaOttawaCanada
  3. 3.Triticeae Research InstituteSichuan Agricultural UniversityChengdu CityChina

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