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Introgression of Xa4, Xa7 and Xa21 for resistance to bacterial blight in thermosensitive genetic male sterile rice (Oryza sativa L.) for the development of two-line hybrids

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Abstract

Bacterial blight (BB) of rice caused by X. oryzae pv. oryzae is a major production constraint in commercial hybrid rice production in the Philippines because most of the parental lines used in hybrid production do not carry resistance genes against the pathogen. In this study, three bacterial blight resistance genes, Xa4, Xa7 and Xa21, were introgressed to a temperature-sensitive genetic male sterile (TGMS1) line. A three-way cross of AR32-19-3-3/TGMS1//IRBB4/7 (PR36944) was made to produce 1,364 F2 plants carrying various combinations of Xa4, Xa7 and Xa21. Individual plants were characterized for reaction to bacterial blight PXO61 (race 1), PXO86 (race 2), PXO99 (race 6) and pollen sterility. Of 144 F2 plants demonstrating resistance against PXO61, PXO86 and PXO99, 22 exhibited highly resistant phenotypes with mean lesion lengths ranging from 0.37–2.97 cm. Analysis of disease reaction identified 20 potential TGMS F2 plants containing Xa4, Xa7 and Xa21 while 78 plants with Xa4 + Xa7. Phenotypic and polymerase chain reaction (PCR) analyses confirmed PR36944-450, PR36944-473 and PR36944-700 as homozygous for Xa7 and Xa21 and highly resistant to all three Xoo races. Fertility of PR36944-450 and PR36944-700 was restored at permissive temperature in a growth chamber. BB-resistant TGMS lines should facilitate breeding two-line hybrids in the tropics.

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Abbreviations

BB:

Bacterial blight

CMS:

Cytoplasmic male sterile

PCR:

Polymerase chain reaction

STS:

Sequence tagged site

TGMS:

Thermo-sensitive genetic male sterile

Xoo :

Xanthomonas oryzae pv. oryzae

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Acknowledgements

This work was conducted as part of the MS Thesis Research Scholarship (LM Perez) provided by the International Rice Research Institute (IRRI) and the Philippine Rice Research Institute (PhilRice).

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Authors and Affiliations

  1. Plant Breeding and Biotechnology Division, Philippine Rice Research Institute, Maligaya, Science City of Munoz, Nueva Ecija, 3119, Philippines

    Loida M. Perez

  2. International Rice Research Institute, DAPO 7777, Metro Manila, Philippines

    Edilberto D. Redoña, Casiana M. Vera Cruz & Hei Leung

  3. Institute of Biological Sciences, University of the Philippines Los Baños, Laguna, 4031, Philippines

    Merlyn S. Mendioro

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  1. Loida M. Perez
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  2. Edilberto D. Redoña
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  3. Merlyn S. Mendioro
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  4. Casiana M. Vera Cruz
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  5. Hei Leung
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Correspondence to Loida M. Perez.

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Perez, L.M., Redoña, E.D., Mendioro, M.S. et al. Introgression of Xa4, Xa7 and Xa21 for resistance to bacterial blight in thermosensitive genetic male sterile rice (Oryza sativa L.) for the development of two-line hybrids. Euphytica 164, 627–636 (2008). https://doi.org/10.1007/s10681-008-9653-1

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