Skip to main content
SpringerLink
Log in

Effect of Benzene on liver functions in rats (Rattus norvegicus)

  • Published:
Environmental Monitoring and Assessment Aims and scope Submit manuscript

Abstract

Rats (Rattus norvegicus) were intraperitoneally injected with a 100 mg kg − 1 dosage of benzene, a toxic and carcinogenic agent widely used for industrial purposes. Changes in lactate dehydrogenase (LDH), alkaline phosphatase (ALP), alanine amino transferase (ALT), aspartate amino transferase (AST) activities in serum of rats were investigated at 2, 4, 8, 16, 32, and 64 h following injection. Serum physiological was administered to control group. Activities were measured using autoanalyzer. Benzene caused significant activations in LDH, ALP, and AST activities in the serum at some test hours (p < 0.05). When compared with the control groups, although an increase occurred in ALT activity, it was seem that this increase wasn’t significant (p > 0.05).

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Aksoy, M. (1980). Different types of malignancies due to occupational exposure to benzene: A review of recent observations in Turkey. Environmental Research, 23, 181–190. doi:10.1016/0013-9351(80)90104-8.

    Article  CAS  Google Scholar 

  • Aksoy, M. (1981). Problem with benzene in Turkey. Regulatory Toxicology and Pharmacology, 1, 147–155, doi:10.1016/0273-2300(81)90091-X.

    Article  CAS  Google Scholar 

  • ASTDR (2007). Agency for toxic substances and disease registry. Toxicological profile for Benzene. U.S. Department of Health and Human Services, Public Health Service (pp. 1–7). Atlanta, GA.

  • Borges, V. C., Rocha, J. B. T., Savegnago, L., & Nogueira, C. W. (2007). Repeated administration of diphenyl ditelluride induces hematological disorders in rats. Food and Chemical Toxicology, 45, 1453–1458. doi:10.1016/j.fct.2007.02.022.

    Article  CAS  Google Scholar 

  • de Paula, F. C. S., Silveira, J. N., Goncalves, J. R., & Alvarez, L. E. M. (2003). Assessment of urinary trans, trans-muconic acid as a biomarker of exposure to benzene. Revista de Saude Publica, 37, 780–785. doi:10.1590/S0034-89102003000600014.

    Google Scholar 

  • Dees, C., Askari, M., & Henley, D. (1996). Carcinogenic potential of benzene and toluene when evaluated using cyclin-dependent kinase activation and p53-DNA binding. Environmental Health Perspectives, 104, 1289–1292. doi:10.2307/3433178.

    Article  CAS  Google Scholar 

  • Dere, E., Giborova, S., & Aydin, H. (2003). The effect of benzene on serum hormones and the activity of some enzymes in different tissues of rats. Acta Veterinaria Beograd, 53, 87–101, doi:10.2298/AVB0303087D.

    Article  Google Scholar 

  • Egeli, Ü., Tunca, B., Tuncel, P., Kahraman, M., Sevinir, B., Çeçener, G., et al. (2000). Genotoxic, hematoxic, pathological, and biochemical effects of hexane on Swiss Albino rats. Teratogenesis, Carcinogenesis and Mutagenesis, 20, 329–340. doi: 10.1002/1520-6866(2000)20:6<329::AID-TCM2>3.0.CO;2-M.

    Article  CAS  Google Scholar 

  • Engelke, M., Bergmann, U., & Diehl, H. A. (1993). Fluidity of the microsomal membrane and cytochrome P-450 reduction kinetics of pig liver microsomes as a consequence of organic solvent impact. Xenobiotica, 23, 71–78.

    Article  CAS  Google Scholar 

  • Farris, G. M., Robinson, S. N., Wong, B. A., Wong, V. A., Hahn, W. P., & Shah, R. (1997). Effects of benzene on splenic, thymic, and femoral lymphocytes in mice. Toxicology, 118, 137–148. doi:10.1016/S0300-483X(96)03606-2.

    Article  CAS  Google Scholar 

  • Glass, D. C., Gray, C. N., Jolley, D. J., Gibbons, C., Sim, M. R., Fritschi, L., et al. (2003). Leukemia risk associated with low-level benzene exposure. Epidemiology, 14, 569–577. doi:10.1097/01.ede.0000082001.05563.e0.

    Article  Google Scholar 

  • Kang, H. G., Jeong, S. H., Cho, M. H., & Cho, J. H. (2007). Changes of biomarkers with oral exposure to benzo(a)pyrene, phenanthrene and pyrene in rats. Journal of Veterinary Science, 8, 361–368.

    Article  Google Scholar 

  • Khan, I. A., Reddy, B. V., Mahboob, M., Rahman, M. F., & Jamil, K. (2001). Effect of phosphorothionate on the reproductive system of male rats. Journal of Environmental Science and Health, Part B, 36, 445–456.

    Article  CAS  Google Scholar 

  • Kolachana, P., Subrahmanyam, V. V., Meyer, K. B., Zhang, L., & Smith, M. T. (1993). Benzene and its phenolic metabolites produce oxidative DNA damage in HL60 cells in vitro and in the bone marrow in vivo. Cancer Research, 53, 1023–1026.

    CAS  Google Scholar 

  • Krewski, D., Snyder, R., Beatty, P., Granville, G., Meek, B., & Sonawane, B. (2000). Assessing the health risks of benzene: A report on the benzene state of the science workshops. Journal of Toxicology and Environmental Health, 61, 307–338. doi:10.1080/00984100050166325.

    Article  CAS  Google Scholar 

  • Levay, G., & Bodell, W. J. (1992). Potentiation of DNA adduct formation in HL-60 cells by combinations of benzene metabolites. Proceedings of the National Academy of Sciences, 89, 7105–7109. doi:10.1073/pnas.89.15.7105.

    Article  CAS  Google Scholar 

  • Lindbohm, M. L. (1995). Effects of parental exposure to solvents on pregnancy outcome. Journal of Occupational and Environmental Medicine, 37, 908–914. doi:10.1097/00043764-199508000-00004.

    Article  CAS  Google Scholar 

  • Lindstrom, A. B., Yeowell-O’connell, K., Waidyanatha, S., Golding, B. T., Tonero-Velez, R., & Rappaport, S. M. (1997). Measurement of benzene oxide in the blood of rats following administration of benzene. Carcinogenesis, 18, 1637–1641. doi:10.1093/carcin/18.8.1637.

    Article  CAS  Google Scholar 

  • Mehlman, M. A. (2002). Carcinogenic effects of benzene; Cesare Maltoni’s contributions. Annals of the New York Academy of Sciences, 982, 137–148.

    Article  CAS  Google Scholar 

  • Ozdikicioglu, F. & Dere, E. (2004). Effects of benzene on glycogen levels of liver and muscle tissues and on blood glucose of rats. Acta Veterinaria Beograd, 54, 379–394. doi:10.2298/AVB0406379O.

    Article  Google Scholar 

  • Rahman, M. F., Siddiqui, M. K., & Jamil, K. (2000). Acid and alkaline phosphatase activities in a novel phosphorothionate (RPR-11) treated male and female rats; evidence of dose and time-dependent response. Drug and Chemical Toxicology, 23, 497–509. doi:10.1081/DCT-100100131.

    Article  CAS  Google Scholar 

  • Robles, H. (1998). Benzene. In: P. Wexler (Ed.), Encyclopedia of toxicology 4 (pp. 133–134). San Diego, CA: Academic Press.

    Google Scholar 

  • Snyder, R. (2000). Overview of the toxicology of benzene. Journal of Toxicology and Environmental Health, 61, 339–346. doi:10.1080/00984100050166334.

    Article  CAS  Google Scholar 

  • Snyder, R., & Hedli, C. C. (1996). An overview of benzene metabolism. Environmental Health Perspectives, 104, 1165–1171. doi:10.2307/3433158.

    Article  CAS  Google Scholar 

  • Sukhodub, A. L., & Padalko, V. I. (1999). Age-dependent changes in rat liver microsomal membrane structure and functions under benzene treatment. Mechanisms of Ageing and Development, 106, 273–282. doi:10.1016/S0047-6374(98)00120-1.

    Article  CAS  Google Scholar 

  • Taskinen, H. K. (1990). Effects of parental occupational exposures on spontaneous abortion and congenital malformation. Scandinavian Journal of Work Environment & Health, 16, 297–314.

    CAS  Google Scholar 

  • Turhan, A., & Dere, E. (2007). The effect of benzene on the activity of adenosine deaminase in tissues of rats. Journal of Biochemistry and Molecular Biology, 40, 295–301.

    CAS  Google Scholar 

  • Turteltaub, K. W., & Mani, C. (eds.) (2003). Research Report 113: Benzene metabolism in rodents at doses relevant to human exposure from urban air (pp. 1–26). Boston MA: Health Effects Institute.

    Google Scholar 

  • Wang, X., & Zhai, W. (1988). Cellular and biochemical factors in bronchoalveolar lavage fluids of rats exposed to fenvalerate. Zhongguo Yaolixue Yu Dulixue Zoghi, 2, 271–276.

    CAS  Google Scholar 

  • Weaver, C. V., Liu, S. P., Lu, J. F., & Lin, B. S. (2007). The effects of benzene exposure on apoptosis in epithelial lung cells: Localization by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) and the immunocytochemical localization of apoptosis-related gene products. Cell Biology and Toxicology, 23, 201–220. doi:10.1007/s10565-006-0165-2.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biology, Faculty of Science and Art, Uludag University, 16059, Nilüfer/Bursa, Turkey

    Egemen Dere & Ferda Ari

Authors
  1. Egemen Dere
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ferda Ari
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Egemen Dere.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Dere, E., Ari, F. Effect of Benzene on liver functions in rats (Rattus norvegicus). Environ Monit Assess 154, 23–27 (2009). https://doi.org/10.1007/s10661-008-0374-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10661-008-0374-7

Keywords

Search

Navigation