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Chromosome mapping of H3 and H4 histone gene clusters in 35 species of acridid grasshoppers

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Abstract

We analyse chromosome location of H3 and H4 histone gene clusters by fluorescence in-situ hybridization (FISH) in 35 species of Acrididae grasshoppers belonging to seven subfamilies. As in other organisms, H3 and H4 co-localized in the same chromosome region in the 11 species where double FISH was performed with the H3 and H4 DNA probes. Chromosome location of H3-H4 histone gene clusters showed high regularity in the species analysed, with all of them carrying a single H3-H4 cluster in an autosome which, in most cases, was located interstitially in the proximal chromosome third. In 17 out of the 21 species with 2n♂ = 23 acrocentric chromosomes, the H3-H4-carrying autosome was about eighth in order of decreasing size. Two of the four exceptions changed H3-H4 localization to proximal (Pezotettix giornae) or distal (Tropidopola graeca) in the eighth-sized autosome, but the remainder (the two Eyprepocnemis species) showed the H3-H4 cluster distally located in the second-sized autosome. All 14 species with 2n♂ = 17 chromosomes (including three long metacentric autosome pairs, five acrocentric autosome pairs and an acrocentric X chromosome) carried an interstitial H3-H4 cluster in the short arm of the smallest of the three long metacentric pairs. These results suggest that chromosome location of H3-H4 histone gene clusters seem to be highly conservative in Acrididae grasshoppers. The change in H3-H4 location from the acrocentric medium-sized autosome in the 2n♂ = 23 karyotype to the long metacentric autosome in the 2n♂ = 17 karyotype is most parsimoniously explained by common ancestry, i.e. by the involvement of the H3-H4-carrying acrocentric in the centric fusion that gave rise to the smallest of the three long metacentric autosomes of 2n♂ = 17 species.

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Abbreviations

BLAST:

Basic Local Alignment Search Tool

CMA3:

chromomycin A3

DAPI:

4′,6-diamidino-2-phenylindole

FISH:

fluorescence in-situ hybridization

NCBI:

National Center for Biotechnology Information

NOR:

nucleolus organizer region

PCR:

polymerase chain reaction

rDNA:

ribosomal DNA

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Acknowledgements

We thank Alexander Bugrov and Dragan Chobanov for sending us fixed materials of Eyprepocnemis unicolor and Tropidopola graeca, respectively, and Walther Traut and two anonymous referees for useful comments. We also thank Tatiana López for technical assistance and David Martínez for English corrections. This study was supported by grants from the Spanish Ministerio de Ciencia y Tecnología (CGL2006-06307) and Plan Andaluz de Investigación (CVI-1664). This research was partially financed by FEDER funds.

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Authors and Affiliations

  1. Departamento de Genética, Universidad de Granada, 18071, Granada, Spain

    Josefa Cabrero, Ma. Dolores López-León, María Teruel & Juan Pedro M. Camacho

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  1. Josefa Cabrero
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  2. Ma. Dolores López-León
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  3. María Teruel
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  4. Juan Pedro M. Camacho
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Correspondence to Juan Pedro M. Camacho.

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Responsible Editor: Walther Traut

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Cabrero, J., López-León, M.D., Teruel, M. et al. Chromosome mapping of H3 and H4 histone gene clusters in 35 species of acridid grasshoppers. Chromosome Res 17, 397–404 (2009). https://doi.org/10.1007/s10577-009-9030-5

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  • DOI: https://doi.org/10.1007/s10577-009-9030-5

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