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Streptomyces ghanaensis pleiotropic regulatory gene wblA gh influences morphogenesis and moenomycin production

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Abstract

The wblA gh gene, encoding a homologue of the WhiB-family of proteins, was identified in the sequenced genome of moenomycin producer Streptomyces ghanaensis. Deletion of the gene blocked aerial mycelium sporulation and caused a 230% increase in moenomycins production. S. ghanaensis overexpressing SSFG-01620: a homologue of extracellular protease inhibitor SCO0762, whose expression in Streptomyces coelicolor is down-regulated by wblA: showed deficiencies in sporulation similar to that of wblA gh knockout strain. The wblA gh gene of S. ghanaensis appears to play a negative role in the control of moenomycin biosynthesis and is essential for sporulation.

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Acknowledgments

This work was supported by grant Bg-01F from Ministry of education, science, youth and sport of Ukraine (to V. F.), and NIH grant 5R01GM076710 (to S. W.). We thank John Santa Maria (Harvard Medical School, USA) for careful reading of the manuscript.

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Authors and Affiliations

  1. Department of Genetic and Biotechnology, Ivan Franko National University of Lviv, Grushevskogo St. 4, Lviv, 79005, Ukraine

    Mariia Rabyk, Bohdan Ostash & Victor Fedorenko

  2. Department of Microbiology and Molecular Genetics, Harvard Medical School, 200 Longwood ave, Boston, MA, 02115, USA

    Yurij Rebets & Suzanne Walker

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  1. Mariia Rabyk
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  2. Bohdan Ostash
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  3. Yurij Rebets
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  4. Suzanne Walker
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  5. Victor Fedorenko
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Correspondence to Victor Fedorenko.

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Rabyk, M., Ostash, B., Rebets, Y. et al. Streptomyces ghanaensis pleiotropic regulatory gene wblA gh influences morphogenesis and moenomycin production. Biotechnol Lett 33, 2481–2486 (2011). https://doi.org/10.1007/s10529-011-0728-z

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  • DOI: https://doi.org/10.1007/s10529-011-0728-z

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