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Genetic factors that influence moenomycin production in streptomycetes

  • Original Paper
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

Moenomycin, a natural phosphoglycolipid product that has a long history of use in animal nutrition, is currently considered an attractive starting point for the development of novel antibiotics. We recently reconstituted the biosynthesis of this natural product in a heterologous host, Streptomyces lividans TK24, but production levels were too low to be useful. We have examined several other streptomycetes strains as hosts and have also explored the overexpression of two pleiotropic regulatory genes, afsS and relA, on moenomycin production. A moenomycin-resistant derivative of S. albus J1074 was found to give the highest titers of moenomycin, and production was improved by overexpressing relA. Partial duplication of the moe cluster 1 in S. ghanaensis also increased average moenomycin production. The results reported here suggest that rational manipulation of global regulators combined with increased moe gene dosage could be a useful technique for improvement of moenomycin biosynthesis.

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Acknowledgments

This work was supported by grant Bg-01F from the Ministry of Education and Science of Ukraine (to V. F.) and NIH grant AI50855 (to S. W.).

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Authors and Affiliations

  1. Department of Genetics and Biotechnology, Ivan Franko National University of L’viv, Grushevskogo st. 4, L’viv, 79005, Ukraine

    Roman Makitrynskyy, Bohdan Ostash, Nestor Zaburannyi, Mariia Rabyk & Victor Fedorenko

  2. Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, MA, USA

    Yuriy Rebets & Suzanne Walker

Authors
  1. Roman Makitrynskyy
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  2. Yuriy Rebets
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  3. Bohdan Ostash
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  4. Nestor Zaburannyi
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  6. Suzanne Walker
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  7. Victor Fedorenko
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Correspondence to Victor Fedorenko.

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Makitrynskyy, R., Rebets, Y., Ostash, B. et al. Genetic factors that influence moenomycin production in streptomycetes. J Ind Microbiol Biotechnol 37, 559–566 (2010). https://doi.org/10.1007/s10295-010-0701-1

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