Abstract
Four yeast strains (RIFY 10001T, RIFY 10002, RIFY 10003, and RIFY 10004) were isolated from flowers growing in fields of mustard and broad beans in Japan. Ascospore formation was not observed. Sequence analysis of the D1/D2 domain of the large subunit ribosomal RNA (LSU rRNA) gene of the four strains indicated that they belong to the genus Metschnikowia and are closely related to Metschnikowia hawaiiana strain CBS 9146T and Metschnikowia orientalis strain CBS 10331T. The D1/D2 domain of the LSU rRNA gene and internal transcribed spacer regions of strain RIFY 10001T were 85.7% identical to those of M. hawaiiana strain CBS 9146T. All four strains were distinguished from the M. hawaiiana strain CBS 9146T by their inability to ferment glucose. Hence, these four strains are novel species and were named as Metschnikowia miensis (holotype: RIFY 10001T; isotypes: NBRC 112445T = CBS 14749T).
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Acknowledgements
We are grateful to Atsushi Yamazaki from Biological Resource Center, National Institute of Technology and Evaluation (NBRC) for assisting with the deposition of the strains.
Funding
This study was supported in part by a research grant from the Institute for Fermentation, Osaka, Japan and by JSPS KAKENHI Grant Number JP26304006 to MO and 17K07837 to CN.
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MO and FY planned the experiments. KS performed the laboratory experiments, analysed the data, and drafted the manuscript. CN contributed to the phylogenetic analysis. MO completed the manuscript and revised the whole manuscript. All authors approved the final version of the manuscript.
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The GenBank/EMBL/DDBJ accession numbers for the D1/D2 domains of the LSU rRNA gene and ITS region of the strain RIFY 10001T are LC337945 and LC339488, respectively, and of the strains RIFY 10002, RIFY 10003, and RIFY 10004 are LC411767–LC411769 and LC411770–LC411772, respectively.
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Shibayama, K., Otoguro, M., Nakashima, C. et al. Metschnikowia miensis f.a., sp. nov., isolated from flowers in Mie prefecture, Japan. Antonie van Leeuwenhoek 113, 321–329 (2020). https://doi.org/10.1007/s10482-019-01340-1
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DOI: https://doi.org/10.1007/s10482-019-01340-1