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Activatable T 1 and T 2 Magnetic Resonance Imaging Contrast Agents

Abstract

Magnetic resonance imaging (MRI) has become one of the most important diagnosis tools available in medicine. Typically MRI is not capable of sensing biochemical activities. However, recently emerged activatable MRI contrast agents (CAs), whose relaxivity is variable in response to a specific parameter change in the surrounding physiological microenvironment, potentially allow for MRI to indicate biological processes. Among the various factors influencing the relaxivity of a CA, the number of inner-sphere water molecules (q) directly coordinated to the metal center, the residence time of the coordinated water molecule (τ m), and the rotational correlation time representing the molecular tumbling time of a complex (τ R) contribute strongly to the relaxivity of an activatable CA. Tuning the ligand structure and properties has been the subject of intensive research for activatable MR CA designs. This review summarizes a variety of activatable MRI CAs sensitive to common variables in microenvironment in vivo, i.e., pH, luminescence, metal ions, redox, and enzymes, etc., with emphasis on the influence of ligand design on parameters q, τ m, and τ R.

Introduction

Since the first inception of X-ray technology for medical imaging over a century ago, many non-invasive imaging methodologies have been developed and applied to biomedical science with the help of the significant advances in electronics, information technology, and nanotechnology.22,73,86 Today imaging has become an indispensable tool in laboratory research, clinical trials, and medical practice. Because of advantages of high spatial resolution, the best soft-tissue contrast in all currently available imaging modalities, low toxicity, and the absence of ionizing radiation, magnetic resonance imaging (MRI) has been singled out as the in vivo diagnostic technology for the next generation.46,84,88

MRI provides anatomical images by measuring proton (1H) relaxation processes of water and soft tissues in biological systems.12 The spinning of the 1H nucleus generates a weak magnetic dipole. In thermodynamic state, all the nuclear dipoles are oriented randomly thus the net magnetic moment is zero. When an external field B 0 is applied, the 1H nuclei experience a torque which forces them “align” with the field in two separate, quantized energy states. The energy difference of the two quantized states (ΔE) is a function of the gyromagnetic ratio of the spin (γ), reduced Planck’s constant \( (\hbar), \) and external magnetic field (B 0) (Eq. 1).

$$ \Updelta E = \gamma \hbar B_{0} $$
(1)

The frequency of the energy (ν) (Eq. 2) required for an NMR transition is commonly represented by Larmor frequency (ω) (Eq. 3).

$$ \nu = \gamma B_{0} /2\pi $$
(2)
$$ \omega = 2\pi \nu = \gamma B_{0} $$
(3)

The energy required for a 1H nuclear spin (γ = 42.576 MHz T−1) transition at currently available external fields (up to 15 T) is at the magnitude of 10−25 J which corresponds to radiowaves in the electromagnetic spectrum. The low energy of NMR means it is a very insensitive technique. When a resonant radio frequency (RF) transverse pulse is perpendicularly applied to B 0, it causes resonant excitation of the magnetic moment precession into the perpendicular plane. Once the RF perturbation is removed, the dipoles return or “relax” to thermodynamic equilibrium. The relaxation behavior of the dipoles is described by longitudinal T 1 relaxation (loss of energy from the excited state to its surroundings, termed as spin–lattice interaction) and transverse T 2 relaxation (random variations in local field strength, caused by neighboring spins, de-synchronize the signals, termed as spin–spin interaction).13

The signal emitted by the hydrogen nuclei during relaxation is referred to as the free-induction decay (FID) response signal. To produce an MRI image, the FID resonance signal must be encoded from each dimension. The encoding in the Z axial direction of B 0 is accomplished by adding a gradient magnetic field to B 0. The gradient causes the Larmor frequency ω to change linearly in the B 0 direction. A tiny axial slice in that direction is then selected and ω of hydrogen nuclei in the slice is assumed to be identical. The spatial reconstruction of each axial slice is realized by using frequency and phase encoding. A gradient G y is applied, causing the resonant frequencies of the nuclei in the slice to vary according to their position in the Y axial direction. G y is then removed and another gradient G x is applied perpendicular to G y. As a result, the resonant frequencies of the nuclei vary in the X axial direction due to G x and have a phase variation in the Y axial direction due to the previously applied G y . Therefore, samples are encoded by frequency in the X axial direction and by phase in the Y axial direction. A Fourier Transform is then used to transform the encoded image to a plane image.13

Intensity of signals from each volume element (voxel) of a given tissue type is a function of proton density of the tissue. The higher the proton density, the stronger the FID response signal. The MR image contrast also depends on tissue-specific parameters T 1 and T 2. When MR images are acquired, the RF pulse is repeated at a predetermined rate. The period of the RF pulse sequence is the repetition time, TR. The time between which the RF pulse is applied and the response signal is measured is the echo delay time, TE. By adjusting TR and TE the acquired MR image can possess contrast for different tissue types.13

Because the endogenous MR differences among various types of tissues can be small, a contrast agent (CA) is often used in MRI to provide additional contrast to distinguish a target tissue from its surroundings. Clinical CAs are in the form of either paramagnetic gadolinium (Gd3+) or manganese (Mn2+) chelates, or superparamagnetic iron oxide (SPIO) and ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles that take effect by catalytically shortening the relaxation times of bulk water protons. Paramagnetic chelates increase the longitudinal relaxation rate (R 1) by roughly the same extent as that they increase the transverse relaxation rate (R 2). They exhibit “positive” (bright) contrast where they localize and are referred to as T 1 agents. In contrast to paramagnetic chelates, iron oxide nanoparticles increase the transverse relaxation rate (R 2) to a much greater extent than they increase the longitudinal relaxation rate (R 1). They exhibit “negative” (dark) contrast where they localize and are referred to as T 2 agents.34

The proportion of clinical MRI protocols using CAs has increased rapidly, from approximately 30% in 1999 to a current estimation of 40–50%.16,85 “Positive” CAs generally are more preferable in the clinic compared to those that induce “negative” contrast or signal loss. It is easier to ascribe signal enhancement, rather than signal loss, to CA accumulation in tissue because there are potentially other sources of signal loss in MR images beyond iron oxide nanoparticle accumulation.25 The majority of MRI CAs currently used in the clinic are chelates of Gd3+ ions, as shown in Fig. 1 and Table 1, because the lanthanide ion has a large magnetic moment and long electronic relaxation time, therefore offering excellent “positive” image contrast.16,38

Figure 1
figure1

Chemical structures including one coordinated water molecule, chemical names, and trademark names of Gd-based MRI CAs approved for clinical use in the EU and USA

Table 1 Properties of clinically approved, Gd-based MRI CAs2,85

The most important property of a CA is its relaxivity (r) that reflects the ability of the CA to generate imaging signal contrast. The r 1 relaxivity of a CA is the agent’s ability to affect the longitudinal relaxation rate r 1 (=1/T 1) of water protons and its value is expressed in mM−1 s−1.77 According to the Solomon–Bloembergen–Morgan (SBM) theory, relaxivity originates from the dipole–dipole interactions between the proton nuclear spins and the fluctuating local magnetic field caused by the unpaired electron spins of the paramagnetic substance.17 Relaxivity depends on a number of factors including the number of inner-sphere water molecules (q) directly coordinated to the metal center, the residence time of the coordinated water molecule (τ m), the rotational correlation time representing the molecular tumbling time of a complex (τ R), electronic parameters, paramagnetic metal ion-proton distance, external field, and temperature.15,17,38,77 The water molecules coordinated to the metal center in Gd-based clinical CAs are defined as the inner-sphere or the first-sphere water molecules (Fig. 2). The Gd CAs have typical values of more than 100 ns for τ m and 0.1 ns for τ R, which results in a low r 1 relaxivity (~5 mM−1 s−1 at 1.5 T) because the optimal values of these agents at 1.5 T should be approximately 10 ns for τ m and at least 10 ns for τ R according to SBM theory. The τ values can be altered via ligand designs that allow faster water exchange and slower molecular tumbling. The tuning of the ligand structure has been the subject of intensive research for the past two decades because an increase in the relaxivity allows for a dramatic decrease in the application dose.15,26,85,87

Figure 2
figure2

Schematic illustration of molecular parameters influencing relaxivity of Gd-based MRI CAs in solution. Image modified from Fig. 2 in Werner et al.

Most of clinically approved Gd CAs are extracellular fluid (ECF) MRI CAs. Their low molecular weight allows them to equilibrate rapidly between the intravascular and interstitial space and then clear almost exclusively by renal filtration on the time scale of a few hours. The ECF CAs are generally non-specific. They do not interact specifically with any type of cells, though their distribution in the body is far from homogeneous.34 MRI with ECF CAs is typically used for a whole body scan, and is not capable of sensing biochemical activities. During the last decade, new types of CAs have been developed—targeted CAs that accumulate in specific tissues and organs and allow for an improved diagnosis of these body regions,41,42,79,82,83 as well as activatable CAs whose relaxivity reports about a specific parameter of the microenvironment in which they distribute and allow for MRI to indicate biological processes.10,27,35,36,41,49,64,67,68,75 Most, though not all, activatable CAs reported are typically small molecular Gd chelates. The principle behind the design of such CAs is that the access of water molecules to the first coordination sphere of the chelated gadolinium ion is controllable through intramolecular rearrangement of the CA, leading to a change in relaxivity. The percent change in relaxivity before and after stimulation is the most important factor in evaluating the efficiency of an activatable MRI CA. Among various factors influencing the relaxivity of a CA, q, τ m, and τ R contribute the most to the relaxivity of an activatable CA.41 Increasing the number of protons coordinated to the paramagnetic ions (q), increasing size, and increasing exchange rate are all known to increase relaxivity.15 Herein, we briefly introduce recent advances in activatable MRI CAs sensitive to common variables in biological microenvironment, i.e., pH, bioluminescence, metal ions, redox, and enzymes.

pH-responsive MRI CAs

Decreased extracellular pH has been observed in cancer and various ischemic diseases and could be a biomarker for early detection or to monitor treatment efficacy.61 Thus, the ability to non-invasively and reliably assess tissue pH is of great interest to scientists and clinicians. One of the earliest and most extensively characterized pH-sensitive MRI CAs, Gd-DOTA-4AmP5−, was developed by Sherry and coworkers.90 The agent, depicted in Fig. 3, consists of Gd3+ chelated by an amido-phosphonate appended derivative of DOTA (1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid). pH sensing for this agent involves an altered proton exchange mechanism due to protonation of the phosphonate appendages that presents a 1.5-fold increase in r 1 over the pH range 9.5–6. The four phosphonate groups have pK as in the range 6.5–8 that, upon protonation, provide hydrogen-bond donors to the Gd3+ bound water molecule allowing catalytic exchange of inner-sphere bound water protons with those of bulk water. A drawback of this system for pH mapping is that the tissue concentration of CA must be known for quantitation.

Figure 3
figure3

Structure of MRI CAs: Gd-DOTA-4AmP, Gd-DOTP, and dual-modality PET/MRI CA: Gd-DOTA-4Amp-F

In response to this obstacle, Gillies and colleagues applied the Gd-DOTA-4AmP5− and a similar, yet pH-insensitive Gd3+-based agent, Gd-DOTP5− (Fig. 3), sequentially (approx 1 h apart), to a rat tumor model.33 With the assumption that the similar CAs had comparable biodistribution and pharmacokinetics, the researchers inferred the concentration of Gd-DOTA-4Amp5− based on the observed concentration of Gd-DOTP5−. The image intensity differences between the agents at maximum enhancement were used to successfully map extracellular tissue pH in the rat glioma model. Another solution for the quantitation dilemma was recently reported by Caravan and coworkers.32 Rather than injecting two CAs, the Gd-DOTA-4AmP5− was modified to incorporate a fluorine radiotracer that allows quantitation via positron emission tomography (PET) imaging. The fluorine modified CA (Fig. 3) retained its pH-responsive relaxivity measuring between 3.9 mM−1 s−1 at pH 6.0 and 7.4 mM−1 s−1 at pH 8.5. Dual-modality imaging of this activatable agent permits simultaneous quantification of the CAs tissue concentration and relaxivity thus providing a region’s pH based on the linear pH-r 1 relationship.

Aime and coworkers investigated a responsive Gd agent that measures pH in a concentration-independent fashion through r 2/r 1 ratiometric analysis.3 The Gd-DOTA functionalized polypeptide (poly-l-ornithine), shown in Fig. 4a, assembles randomly at low pH or into an ordered helix at higher pH; this pH-dependent change in conformation affects the rotational mobility of the complex yielding variation in the r 2/r 1 ratio. Similarly, Sherry and colleagues reported an MRI CA that utilizes altered molecular tumbling and proton exchange kinetics to magnify pH sensitivity.4 The relaxivity and responsiveness of the previously discussed, small-molecule agent, Gd-DOTA-4AmP5−, was improved upon through conjugation to the surface of a PAMAM dendrimer (Fig. 4b) thus producing a macromolecular sensor with ~96 pH-responsive Gd chelates. This approach affecting both molecular tumbling and exchange kinetics of inner- and outer-sphere water molecules provided increased overall relaxivity by a factor of nearly 5 and enhanced pH responsiveness for the dendrimer by means of a greater amplitude change in r 1 (a 2.2-fold increase was observed over the pH range 9.5–6) over the measured pH range. While the large size and ~140 kDa molecular weight of the agent positively affected its pH response, they may prove detrimental to its effectiveness in vivo. Dendrimers have the potential for toxicity due to slow clearance through the liver, and they may not diffuse from the vasculature as easily as small molecule CAs. Further experiments are needed to assess pharmacokinetics and biodistribution of the dendrimer based CA.

Figure 4
figure4

Structures of the macromolecular, pH-sensitive CAs (a) (Gd-DOTAam)33-Orn205 and (b) Gd-DOTA-4AmP-PAMAM dendrimer. Graphic (a) modified from Chart 1 in Aime et al. 3 Graphic (b) modified from Ali et al. 4

Two unconventional, Gd-based pH-responsive CAs were reported by Wilson, Toth, and coworkers in the form of gadofullerenes76 and gadonanotubes.37 The water-soluble gadofullerenes, Gd@C60[C(COOH)2]10 and Gd@C60(OH) x , were shown to exhibit a pH-dependent reversible aggregation that results in variation of the rotational correlation time and thus variable relaxivity. As pH was decreased, the researchers observed aggregation of the agent by dynamic light scattering (DLS) size measurement. Correspondingly, the proton relaxivities of Gd@C60[C(COOH)2]10 and Gd@C60(OH) x increase by a factor of 3.8 and 2.6, respectively, with decreasing pH over the range 12–2. These gadofullerenes offer two distinct advantages towards translating them into clinically viable MRI CAs for pH sensing. First, water-soluble fullerenes have shown the ability to cross cell membranes, and second, the entrapment of Gd3+ within the fullerene cage prevents the toxicity observed from free, unchelated Gd3+ ion. Gadonanotubes, represented in Fig. 5, are 20–80 nm segments of single-walled carbon nanotubes that have sidewall defects in which 3–10 Gd3+ ions cluster. The short nanotubes were prepared by a chemical cutting process that creates defects in which Gd3+ ions are loaded with chloride as counter ions. The gadonanotubes display remarkably high relaxivity (180 mM−1 s−1 at 1.5 T) compared to current clinically used MRI CAs. In addition, the authors report a >3-fold increase in relaxivity observed from pH 8.3 to 6.7. While the mechanism of the pH-dependent contrast enhancement is unclear, experiments verified that Gd3+ ion are not leaked from the nanotube clusters upon pH cycling or physiological challenges such as phosphate buffer solution (PBS), bovine serum, or heat. These results show potential for development of gadonanotubes as efficient, ultra-sensitive pH-responsive MRI CAs.

Figure 5
figure5

Pictorial representation of Gd3+ ions ( ) within the sidewall defects of a nanotube. Gd clusters measure roughly 1 × 5 nm with 3–10 Gd3+ ions per cluster. Graphic from Fig. 1 in Hartman et al. 37

Light-Responsive MRI CAs

One of the more recent and novel advances in the field of activatable MRI probes is the development of light-responsive CAs. Bioluminescence imaging has found wide use in cell and molecular biology. Scientists and clinicians are able to use luminescence from markers such as green fluorescent protein or the luciferase system as reporters of gene expression or for cell-tracking purposes.28 While optical imaging of bioluminescence offers the benefit of high sensitivity, it suffers the drawback of limited tissue penetration depth. Therefore, a photo-responsive, reversibly activatable MRI CA would provide a means of imaging such bioluminescence in deep tissues. Louie and coworkers have developed a series of MRI CAs that respond to visible and UV light. Utilizing light-responsive molecular switches from the spiropyran family of compounds, they have developed both T 1 78,81 and T 2 57 CAs that can modulate MR signal with light stimulus.

The T 1 agents consisted of either a single nitro or dinitro-spiropyran derivative that was tethered to a DO3A chelated Gd3+ ion. Varying degrees of light response were observed depending on the spiropyran moiety that the agent possessed. The “single nitro” version showed a 21% decrease in relaxivity (3.72–2.93 mM−1 s−1) in solution studies upon visible light irradiation following storage in the dark. Whereas, the dinitrospiropyran-Gd-DO3A presented an 18% decrease in relaxivity with white light stimulus (2.51–2.05 mM−1 s−1). Both agents exhibited reversibility with UV irradiation (at 365 nm) or storage in the dark. The observed r 1 modulation was attributed to a change in hydration state, q, of the Gd3+ ion due to the ability of the tethered spiropyran to block water access upon absorbance of visible light. Beyond the light stimulated response, the authors note that the dinitrospiropyran-GdDO3A agent also possessed a novel NADH response that is discussed further in “Redox potential responsive MRI CAs” section of this review.

Most recently, the Louie group reported an activatable MRI T 2 agent, CLADIO-NH-SP, that modifies relaxation time through reversible aggregation of magnetic nanoparticles (Fig. 6).57 This probe employs a spiropyran derivative that is covalently bound to the surface of cross-linked and aminated dextran sulfate coated iron oxide nanoparticles. The spiropyran derivative changes conformation reversibly between hydrophobic and hydrophilic isomers with UV and visible light irradiation; this conformation switching directs the aggregation and dispersion of the nanoparticles, thus regulating T 2 relaxation time. The authors observed nanoparticle aggregation by DLS size measurement and a 34% decrease in T 2 relaxation time upon visible light irradiation. In addition, the reported absorbance maximum of the spiropyran terminated CA is 563 nm which provides overlap with the emission of firefly luciferin; therefore, this light-responsive MRI probe shows promise for development as a non-invasive reporter of gene expression.

Figure 6
figure6

Mechanism of reversible aggregation for light-responsive CLADIO-NH-SP. Image from Scheme 1 in Osborne et al. 57

Metal ion-responsive MRI CAs

Metal ions play significant roles in biological signaling events, redox homeostasis, and metabolism. Therefore, it is not surprising to see research effort in the last decade towards development of activatable MRI agents responsive to biologically relevant metal ions such as Ca2+, Zn2+, Fe2+, and Cu+/2+ ions. Meade and colleagues reported the first Ca2+-responsive MRI CA consisting of two Gd-DO3A chelates connected by a modified BAPTA (1,2-bis(o-aminophenoxyl)-ethane-N,N,N′,N′-tetreaacetic acid) linker (Fig. 7).45 In the absence of Ca2+, the carboxylate arms of the BAPTA moiety bind the chelated Gd3+ yielding a relaxivity of 3.26 mM−1 s−1. In the presence of Ca2+, the BAPTA selectively binds Ca2+ and frees up Gd3+ binding sites for bulk water, resulting in a relaxivity increase to 5.76 mM−1 s−1. The agent’s BAPTA linker possesses a preference for Ca2+ over Mg2+ with a binding affinity in the low micromolar range which makes this CA capable of detecting intracellular Ca2+. Since the Meade group contribution, several other lower affinity Ca2+-sensitive CAs have been reported that utilize dual Gd-DO3A chelates with varying linkers such as BAPTA bisamide,29 EDTA,53 DTPA,53 and EGTA.5 With millimolar binding affinities, these agents show promise as extracellular Ca2+ sensors.

Figure 7
figure7

Ca2+-sensitive MRI CAs: (a) BAPTA linked dual Gd-DO3A; (b) Gd-DOPTRA; and (c) Gd–do3apBP

Dhingra, Logothetis, and coworkers report an MRI CA, Gd-DOPTRA (Fig. 7), which provides nearly 100% contrast enhancement upon coordination of Ca2+ (3.5 mM−1 s−1 in the absence of Ca2+ and 6.9 mM−1 s−1 with addition of Ca2+).29 Gd-DOPTRA is composed of a Gd-DO3A unit linked to a micromolar affinity Ca2+ chelator, APTRA (o-amino-phenol-N,N,O-triacetate). This agent offers the benefits of micromolar Ca2+ binding in a more condensed structure than those previously developed; however, specificity for Ca2+ is to some extent compromised as this agent produces relaxivity changes in response to Mg2+ and Zn2+, as well. Kubicek et al. recently reported another activatable CA, Gd-do3apBP, constructed of a single Gd-DO3A core with a bisphosphonate arm (Fig. 7).44 The bisphosphonate group coordinates the divalent metal ions Ca2+, Mg2+, and Zn2+ in an apparent coordination oligomer resulting in increases in r 1 up to 200–500%. This staggering relaxivity enhancement is observed upon addition of 3 equiv. of metal ions to a 2 mM CA solution in a pH-dependent fashion that varies with the metal species. The authors note that the Zn2+ concentration used is not in the biologically relevant range (<0.1 mM), though Ca2+ and Mg2+ sensing is feasible though specificity is not yet ideal.

Beyond the T 1 Gd-based probes, Jasanoff and coworkers report calmodulin (CaM) modified superparamagnetic iron oxide (SPIO) nanoparticles as Ca2+-responsive MRI T 2 agents.6 CaM, a calcium signaling protein, binds target peptides in response to Ca2+ levels. By applying CaM- and peptide-conjugated SPIOs (20–100 nm) in a 3:1 ratio, the researchers observed significant changes in T 2 relaxivity through aggregation of the SPIOs resulting from Ca2+ induced cross-linking of CaM and target peptides. This CA was found to detect micromolar Ca2+ concentration in an appropriate range for intracellular Ca2+ sensing. Furthermore, the sensitivity can be manipulated by structurally modifying the CaM or target peptides. Currently, the most significant hurdle to biological application of the existing agent is the inability to cross cellular membranes. Further surface modification is required to address this issue.

Similar to those of Ca2+, Gd-DO3A based agents selectively responsive to Zn2+ have also been developed. The Meade group produced two MRI CAs with >100% increases in relaxivity upon Zn2+ detection. Gd-daa350 and Gd-apa348 provide selective Zn2+ binding over other biologically abundant cations such as Ca2+, Mg2+, Na+, and K+. Recently, the Zn2+-responsive CA, Gd-DOTA-diBPEN, has been found to increase in relaxivity 165% (6.6–17.4 mM−1 s−1) due to an interaction with human serum albumin (HSA).31 In the absence of HSA, the relaxivity increase is a modest 20% upon Zn2+ detection, suggesting τ R modulation. Gd-DOTA-diBPEN does not respond to Ca2+ or Mg2+, yet can detect Zn2+ down to 30 μM in the presence of HSA as observed in in vitro imaging studies.

Though iron-sensing CAs have not been characterized in literature, several Gd chelates that also coordinate Fe with corresponding relaxivity increases have been reported.1,24,58,59 For instance, Parac-Vogt et al. describe an MRI probe, Gd-DTPA-phen, that self-assembles 3:1 with an Fe2+ ion.58 The supramolecular complex, [(Gd-DTPA-phen)3Fe], has a relaxivity of 9.5 mM−1 s−1 as compared to 3.9 mM−1 s−1 for Gd-DTPA. Similarly, Desreux and coworkers reported a 77% increase in relaxivity upon coordination of Fe2+ with three GdPhenHDO3A complexes.59 Neither of these cases address the sensitivity or specificity for Fe2+ over other metal ions so further exploration into their suitability as Fe-sensitive CAs is needed.

Cu-sensing agents are among the newest metal ion-responsive MRI CAs. Chang and coworkers recently reported a series of sensors that utilize a Gd-DO3A core with a Cu ion binding thioether pendant arm (Fig. 8).65 These agents provide r 1 modulation through altered hydration state of the Gd3+ ion. In the absence of copper, the pendant arm appears to block water access to the Gd core yielding q = 0.3; upon addition of 1 equiv. Cu+, q increases to 2. The most responsive of these agents yields a 360% increase in relaxivity upon Cu+ binding (1.5–6.9 mM−1 s−1 with Cu+). In addition, the Chang group has developed a sensor that can detect Cu2+ with high affinity through inclusion of an O donor in the pendant arm (Fig. 8c).65 These CAs offer significant benefits over those previously reported for Cu2+ detection in that they possess picomolar range Cu+/Cu2+ binding affinity with high specificity over other biologically endogenous metal ions. In the realm of responsive T 2 agents, Patel et al. reported SPIO nanoparticles surface-modified with DOTA for the chelation of Cu2+.60 These agents were developed as dual-modality PET/MRI CAs, but a strong increase in r 2 relaxivity (~300%) upon coordination of Cu2+ ions points to potential use as activatable MRI CAs responsive to micromolar Cu2+ concentration. The authors speculate as to the mechanism causing r 2 increase, but indicate that aggregation of the nanoparticles is not observed. The association of water molecules at the DOTA bound Cu2+ ion may facilitate transmission of the magnetic induction of the SPIO to bulk water, or the surface modification could possibly shorten the residence lifetime of coordinated water.

Figure 8
figure8

Gd-DO3A-based MRI CA. (a) with a Cu+-binding thioether pendant arm demonstrating; (b) r 1 relaxivity increases (with corresponding percent increase given) to 1 equiv. of Cu+ in the presence of various biological anions. White bars represent CA relaxivities in the absence of Cu+ while the black bars represent relaxivities with addition of Cu+ (60 MHz, 37 °C, pH = 7.4); (c) shows the Cu2+ activated CA. Graph from Fig. 7 in Que et al. 65

Redox potential-responsive MRI CAs

Redox reactions are widely seen in biochemical activities. Disruption of redox homoeostasis may lead to many pathological conditions, such as atherosclerosis, stroke, Alzheimer’s disease, Parkinson’s disease, and cancer, etc.39 However, non-invasive observation of intracellular redox activities and their relationship to physiological function is still a challenge for molecular imaging, most likely due to the lack of appropriate probes responsive to redox potential.14,40,51

Louie and co-workers have developed activatable MRI CAs sensitive to reduced nicotinamide adenine dinucleotide (NADH), the principal electron donor for the respiratory chain in mammalian cells.43,80,81 In the first generation agent a spirobenzopyran molecule, which is responsive to changes in light and electrical activities,7,63,91 was coupled to Gd-DO3A (DO3A: 1,4,7,10-tetraazacyclododecane-1,4,7-trisacetic acid). As described earlier in the paper, when photo-stimulation was applied, the structural change of the spirobenzopyran moiety in the resultant agent caused the two isomers to have different contrast enhancement properties for MRI. However, the CA was not responsive to electrical activity or redox potential.78 By adding a strong electron-withdrawing nitro group to the indole ring, the r 1 relaxivity of the resultant dinitrospiropyran-Gd-DO3A is found to be responsive to both light and NADH. When in the dark, the CA is in its open (MC) form and has an r 1 relaxivity of 2.51 mM−1 s−1. After irradiation with visible light or mixing with NADH, the CA experiences a color change and its r 1 relaxivity decreases to 2.05 and 1.86 mM−1 s−1, respectively.81 The second generation agent employs spironaphthoxazines instead of spirobenzopyran.23,89 In the dark the agent has an r 1 relaxivity of 5.58 mM−1 s−1 and an average of 1.26 water molecules coordinated with the Gd(III). In the presence of NADH, as shown in Fig. 11, the agent undergoes an isomerization and its hydration number (q) increased to 2.01, which leads to an r 1 relaxivity increase of 54%. The T 1 value can be fully restored after applying hydrogen peroxide to the system. Macrophages are incubated with spirooxazine-Gd-DO3A, then exposed to externally applied NADH. Cells exposed to NADH show substantial contrast enhancement, as shown in Figs. 9a and 9b.

Figure 9
figure9

The structural and hydration number (q) changes in the first coordination sphere of Gd(III) of spirooxazine-Gd-DO3A triggered by NADH and hydrogen peroxide. T 1-weighted MRI images of (a) spirooxazine-Gd-DO3A and (b) the mixture of spirooxazine-Gd-DO3A and NADH (Gd:NADH = 1:1), in P388D1 murine macrophages. Images modified from Scheme 2 and Fig. 5 in Tu et al. 80

Unlike the Gd agents described above whose redox reactions take place on the ligand, redox reactions of activatable iron (Fe) agent occur directly at the metal center. Jasanoff and coworkers report injection of iron-containing hemoglobin (Hb) into blowflies; they found that the Hb can permeate through relatively dense neural tissue without obvious disruption to physiology and the oxidation state of Hb is dependent on the partial pressure of oxygen, pO2. Oxygen is an important parameter in living systems. Variations in pO2 have been relevant to many diseases, such as stroke and tumors.52,62 Hb-injected flies show approximate 40–50% changes in signal intensity when external O2 levels are manipulated artificially from 0 to 21%, because the iron in the porphyrin ring of Hb changes in redox state from diamagnetic Fe(II) to paramagnetic Fe(III) in the presence of O2. In vivo T 2-weighted MRI shows that a small amount of Hb is able to produce substantial pO2-dependent signal contrast, and the contrast changes can be roughly calibrated if necessary.74

Enzyme-responsive MRI CAs

The enzymatically modulated MRI CAs could provide an effective means of measuring enzyme activity, detecting enzyme location, and assaying gene expression. Meade and coworkers developed the first example of enzyme-sensitive MRI CA and demonstrated the principle of enzymatic activation of an MRI CA by beta-galactosidase.47,54 More recently, Meade group developed a Gd-DO3A derivative which shows relaxivity change in the presence of β-glucuronidase.30 As shown in Fig. 10, the agent bears a pendant β-glucuronic acid moiety, the substrate of β-glucuronidase, on an arm of DO3A. In the presence of bovine liver β-glucuronidase, an ether bond of the agent was enzymatically cleaved, resulting in a self-immolating reaction passing through the spacer until the breakage of the amide bond (red arrow), and restricting water access to the paramagnetic ion. In comparison with the original beta-galactosidase agent, the resultant 2-aminoethyl-Gd-DO3A has an extra amine group (blue arrow) coordinated to the metal center, thus is expected to have a lower q number and relaxivity value. However, the relaxivity changes of the enzymatic hydrolytic reaction markedly depended on buffer composition employed: the relaxivity increased in a buffer mimicking in vivo anion concentrations by 17%, while the relaxivity decreased by 27% for the same experiment in human blood serum.

Figure 10
figure10

Schematic illustration of β-glucuronidase-catalyzed cleavage of a Gd-DO3A type chelate bearing the substrate of β-glucuronidase

Besides hydrolases, oxidoreductases also have been targets in the exploration of enzyme-sensitive paramagnetic CAs. A MR signal amplification strategy has been developed based on oxidoreductase-mediated polymerization of paramagnetic Gd substrates into oligomers of higher magnetic relaxivity.8,9,11,1821,55,66,67,6972 Benzene-1,2-diol which is known to be a substrate of oxidized peroxidases was linked to a Gd-DOTA chelate, and the resultant agent acted as a monomer (Fig. 11a). In the presence of peroxidase, the monomers were induced to form radicals that trigger the rapid condensation into paramagnetic oligomers leading to a threefold increase in r 1 relaxivity due to an increase in τ R. By this signal amplification mechanism, horseradish peroxidase (HRP) could be detected in the nanomolar range. In vivo MRI was also performed to detect E-selectin expression on human endothelial cells in culture by using a highly specific first antibody, followed by a secondary antibody conjugate with HRP.8

Figure 11
figure11

Schematic illustration of Gd chelates sensitive to myeloperoxidase (MPO)

By substituting the catechol moiety with a serotonin moiety (Fig. 11b), the Gd(III) chelate was oriented to be responsive to myeloperoxidase (MPO), a key-marker of inflammation processes involved in numerous pathologies. Serotonin is a naturally occurring neurotransmitter that functions as a reducing substrate for MPO. The agent is efficiently polymerized in the presence of human neutrophil MPO resulting in a 70–100% increase in proton relaxivity.20 The MPO-enhanced MRI signal was also obtained when using Gd-DTPA bearing either tyramide (Fig. 11c or 5-hydroxytryptamide-(serotonin) (Fig. 11d) groups.70 The activation involves the formation of a radical species that can proceed differently depending on the considered complex. Two mechanisms have been suggested.71 One is related to the oligomerization of the complex and the other is related to the formation of cross-linked structures with proteins, as shown in Fig. 12. However, it is also found that sometimes the Gd agent may utilize both mechanisms when activated.71

Figure 12
figure12

The activation mechanism of the Gd agent by the MPO–H2O2 system. The MPO-sensitive moiety in the gadolinium complex can be oxidized by MPO in the presence of H2O2 to generate a radical intermediate. The radicals can form dimers and oligomers with other radicals, or can bind to other phenolic residues on the protein surface. Both activation effects will decrease τ R, resulting in higher r 1 relaxivity. Images modified from Scheme 2 in Rodriguez et al. 71

The Gd-DTPA-serotonin, referred as MPO-Gd, was further applied for in vivo MRI of ischemic injury of the myocardium which causes timed recruitment of neutrophils and monocytes/macrophages, which produce substantial amounts of local MPO.55 Myocardial infarction (MI) was induced by left coronary artery ligation of normal (C57BL/6) mice.56 After 2 days coronary ligation, mice were scanned before and up to 2 h after injection of equal doses of MPO-Gd and control Gd-DTPA. The signal enhancement was observable in the kinetic infarct at 10 and 30 min after injection of Gd-DTPA with peak enhancement at 10 min, then completely returned to the original status after 60 min. After injection of MPO-Gd, the peak enhancement appeared at 60 min and the signal intensity was significantly higher in comparison with that of Gd-DTPA. The enhancement was still observable even at 120 min after injection.55

Concluding Remarks

During the last decade, emergence of suitable CAs which report on physiological processes has expanded the scope of MRI beyond anatomical and functional imaging to also convey information at the cellular or even molecular level. Though a variety of publications have shown the great potential of activatable CAs to report about changes of diagnostically relevant variables of physicochemical microenvironments in vitro, so far only very few examples have moved into in vivo studies. An active and effective collaboration between chemists, biologists, and clinicians is particularly critical in bringing activatable CAs with outstanding properties into realistic uses. Feedback from biologists, imagers, and clinicians will be crucial for chemists to design more versatile and controllably activatable CAs whose structures can be readily and finely tuned to undergo either intramolecular transitions or intermolecular interactions resulting in greater relaxivity changes in response to variables of physiological microenvironments.

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Acknowledgments

The authors wish to acknowledge the National Institute of Health (HL081108-01, EB008576-01, and EB006192) and the NMR award of the University of California, Davis for support of this work.

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Correspondence to Angelique Y. Louie.

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Elizabeth A. Osborne—co-first author.

Associate Editor Daniel Takashi Kamei oversaw the review of this article.

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Open Access This is an open access article distributed under the terms of the Creative Commons Attribution Noncommercial License (https://creativecommons.org/licenses/by-nc/2.0), which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.

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Tu, C., Osborne, E.A. & Louie, A.Y. Activatable T 1 and T 2 Magnetic Resonance Imaging Contrast Agents. Ann Biomed Eng 39, 1335–1348 (2011). https://doi.org/10.1007/s10439-011-0270-0

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Keywords

  • Magnetic resonance imaging (MRI)
  • Contrast agent
  • Relaxivity
  • Gadolinium
  • Activatable
  • Responsive