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Preparation of Novel Zwitterionic Monolith for Capillary Electrochromatography and Nano LC–MS Applications

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Abstract

Herein, a zwitterionic organic polymer monolithic stationary phase was prepared based on the in situ thermal-initiated copolymerizing 3-dimethyl-(3-(N-methacrylamido) propyl) ammonium propane sulfonate (DMMPPS) and pentaerythritol triacrylate (PETA) in a binary porogenic solvent consisting of MeOH and H2O. An HILIC/RP dual separation mechanism was observed on this optimised poly(DMMPPS-co-PETA) monolithic column and the composition of the mobile phase corresponding to the transition from the HILIC to the RP mode was around 30% ACN in water. The proposed monolithic column was successfully applied to separate 12 polar neurotransmitters in a nano LC–MS mode. About ~ 27 μm plate height (corresponding to column efficiency of ~ 93,000 plates/m) was obtained at the linear velocity of 1 mm/s. Meantime, the DMMPPS-based monolith exhibited good mechanical stability and excellent separation performance for nucleic acid bases, nucleosides and nucleotides for capillary electrochromatography (CEC). Relative standard deviations (RSD%) of the retention times for five nucleic acid bases and nucleosides were in the range of 0.15–0.42% (run-to-run, n = 3) and 2.63–4.50% (column-to-column, n = 3), respectively. Our work demonstrated that the zwitterionic monolith columns could be an effective separation tool for analysis of hydrophilic substances both on the HI-CEC and nano LC–MS mode.

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Acknowledgements

This research project was financed by the Xi’an Science and Technology Plan Project (2019KJWL02), the Natural Science Foundation of Shaanxi Province (2018JQ2024), the Special Scientific Research Project of Shaanxi Provincial Education Department (17JK1127) and Shaanxi college students’ innovation and entrepreneurship training project (S201911080054).

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Authors and Affiliations

  1. School of Chemical Engineering, Xi’an University, Xi’an, 710065, China

    Feng Li & Jingjing He

  2. State Key Laboratory of Bioorganic and Natural Products Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai, 200032, China

    Danye Qiu & Jingwu Kang

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  1. Feng Li
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  2. Danye Qiu
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  3. Jingjing He
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Correspondence to Feng Li.

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Li, F., Qiu, D., He, J. et al. Preparation of Novel Zwitterionic Monolith for Capillary Electrochromatography and Nano LC–MS Applications. Chromatographia 83, 115–122 (2020). https://doi.org/10.1007/s10337-019-03823-9

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