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Preparation of a Sulfoalkylbetaine-Based Zwitterionic Monolith with Enhanced Hydrophilicity for Capillary Electrochromatography Separation Applications

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Abstract

A novel monolithic stationary phase based on in situ copolymerization of zwitterionic monomer N,N-dimethyl-N-methacryloxyethyl-N-(3-sulfopropyl) ammonium betaine (DMMSA), pentaerythritol triacrylate (PETA), either methacrylatoethyl trimethyl ammonium chloride (META) or sodium 2-methylpropene-1-sulfonate (MPS) was designed as a multifunctional separation column for hydrophilic interaction capillary electrochromatography (HI-CEC). A significantly enhanced hydrophilicity was obtained on the poly(DMMSA-co-PETA-co-META or MPS) monolith, which was contributed by the high percentage of DMMSA in the polymerization mixture. A column efficiency of 200,000 plates/m was obtained and the monolithic column also displayed a satisfactory repeatability in terms of migration time with RSD values less than 1.1% (intra-day, n = 5) and 2.0% (inter-day, n = 3). Most importantly, the column was successfully applied to separation of a pool of neurotransmitters which are not well separated on commercial HILIC packing materials. A baseline separation of the 12 model components was obtained with good selectivity, symmetrical peak shape and high column efficiency with BGE consisting of 20 mM ammonium formate (pH 3.0) in ACN/H2O (80/20, v/v).

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Fig. 1

Redrawn from Ref. [13] with permission of the publisher

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Acknowledgements

This work was financially supported by the National Natural Science Foundations of China (21375140, 21175146) and the Strategic Priority Research Program of the Chinese Academy of Sciences (Grant No. XDB20020200).

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Author notes

  1. Feng Li and Danye Qiu contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory for Surface Engineering and Remanufacturing of Shanxi Province, School of Chemical Engineering, Xi’an University, Xi’an, 710065, China

    Feng Li

  2. State Key Laboratory of Bioorganic and Natural Products Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai, 200032, China

    Danye Qiu & Jingwu Kang

Authors
  1. Feng Li
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  2. Danye Qiu
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  3. Jingwu Kang
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Correspondence to Feng Li.

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The authors declare no competing financial interest.

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Li, F., Qiu, D. & Kang, J. Preparation of a Sulfoalkylbetaine-Based Zwitterionic Monolith with Enhanced Hydrophilicity for Capillary Electrochromatography Separation Applications. Chromatographia 80, 975–981 (2017). https://doi.org/10.1007/s10337-017-3303-5

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