Abstract
Objective
The purpose of this paper was to study the expression levels of newly described lymphatic endothelial markers — LYVE-1, Prox-1, podoplanin and 5′-nucleotidase, and their correlation with metastasis of human colorectal cancers.
Methods
Tumor and corresponding tumor-side normal tissue samples were obtained from resected specimens immediately after operation. Expression level of each factor was determined by quantitative real-time PCR (RT-PCR) and Western blot technique.
Results
Expression levels of lymphatic endothelial markers LYVE-1, Prox-1, podoplanin and 5′-nucleotidase were significantly different in tumor and tumor-side normal groups. Expression levels of Prox-1 and podoplanin were higher in patients with positive lymph node metastasis than those without metastasis. LYVE-1, but not 5′-nucleotidase expression level was higher in both cancer and normal groups.
Conclusion
These results indicate that combined quantitative analysis of lymphangiogenic markers LYVE-1, Prox-1 and podoplanin in colorectal cancer specimens may be useful in predicting metastasis of colorectal cancer to regional lymph nodes. However, the role of 5′-nucleotidase in predicting metastasis of colorectal cancer still remains to be further analyzed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Vittet D, Feige JJ. Lymphangiogenesis and tumor progression. Bull Cancer (French), 2007, 94: 881–886.
Stacker SA, Hughes RA, Williams RA, et al. Current strategies for modulating lymphangiogenesis signalling pathways in human disease. Curr Med Chem, 2006, 13: 783–792.
Dadras SS, Detmar M. Angiogenesis and lymphangiogenesis of skin cancers. Hematol Oncol Clin North Am, 2004, 18: 1059–1070.
Shayan R, Achen MG, Stacker SA. Lymphatic vessels in cancer metastasis: bridging the gaps. Carcinogenesis, 2006, 27: 1729–1738.
McColl BK, Loughran SJ, Davydova N, et al. Mechanisms of lymphangiogenesis: targets for blocking the metastatic spread of cancer. Curr Cancer Drug Targests, 2005, 5: 561–571.
Dadras SS, Skrzypek A, Nguyen L, et al. Prox-1 promotes invasion of kaposiform hemangioendotheliomas. J Invest Dermatol, 2008, 128: 2798–2806.
Raica M, Cimpean AM, Ribatti D. The role of podoplanin in tumor progression and metastasis. Anticancer Res, 2008, 28: 2997–3006.
Whitehurst B, Eversgerd C, Flister M, et al. Molecular profile and proliferative responses of rat lymphatic endothelial cells in culture. Lymphat Res Biol, 2006, 4: 119–142.
Nakamura Y, Yasuoka H, Tsujimoto M, et al. Importance of lymph vessels in gastric cancer: a prognostic indicator in general and a predictor for lymph node metastasis in early stage cancer. J Clin Pathol, 2006, 59: 77–82.
Ji RC. Lymphatic endothelial cells, tumor lymphangiogenesis and metastasis: new insights into intratumoral and peritumoral lymphatics. Cancer Metastasis Rev, 2006, 25: 677–694.
Kato S, Shimoda H, Ji RC, et al. Lymphangiogenesis and expression of specific molecules as lymphatic endothelial cell markers. Anat Sci Int, 2006, 81: 71–83.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
He, J., Lv, Y. The quantitative expressions of lymphangiogenic factors and metastasis in human colorectal cancers. Chin. -Ger. J. Clin. Oncol. 10, 170–173 (2011). https://doi.org/10.1007/s10330-011-0756-0
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10330-011-0756-0