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Effect of soya lecithin on the enzymatic system of the white-rot fungi Anthracophyllum discolor

  • M. Bustamante
  • M. E. González
  • A. Cartes
  • M. C. DiezEmail author
Original Paper

Abstract

The present work optimized the initial pH of the medium and the incubation temperature for ligninolytic enzymes produced by the white-rot fungus Anthracophyllum discolor. Additionally, the effect of soya lecithin on mycelial growth and the production of ligninolytic enzymes in static batch cultures were evaluated. The critical micelle concentration of soya lecithin was also studied by conductivity. The effects of the initial pH (3, 4, and 5) and incubation temperature (20, 25, and 30°C) on different enzymatic activities revealed that the optimum conditions to maximize ligninolytic activity were 26°C and pH 5.5 for laccase and manganese peroxidase (MnP) and 30°C and pH 5.5 for manganese-independent peroxidase (MiP). Under these culture conditions, the maximum enzyme production was 10.16, 484.46, and 112.50 U L−1 for laccase, MnP, and manganese-independent peroxidase MiP, respectively. During the study of the effect of soya lecithin on A. discolor, we found that the increase in soya lecithin concentration from 0 to 10 g L−1 caused an increase in mycelial growth. On the other hand, in the presence of soya lecithin, A. discolor produced mainly MnP, which reached a maximum concentration of 30.64 ± 4.61 U L−1 after 25 days of incubation with 1 g L−1 of the surfactant. The other enzymes were produced but to a lesser extent. The enzymatic activity of A. discolor was decreased when Tween 80 was used as a surfactant. The critical micelle concentration of soya lecithin calculated in our study was 0.61 g L−1.

Keywords

Soya lecithin White-rot fungi Anthracophyllum discolor Ligninolytic enzymes Phytogenic surfactant 

Notes

Acknowledgments

This work was supported by FONDECYT 1090678 and partially by Dirección de Investigación Universidad de La Frontera (DIURO).

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Copyright information

© Society for Industrial Microbiology 2010

Authors and Affiliations

  • M. Bustamante
    • 1
  • M. E. González
    • 1
  • A. Cartes
    • 2
  • M. C. Diez
    • 3
    Email author
  1. 1.Doctoral Program in Sciences of Natural ResourcesUniversity of La FronteraTemucoChile
  2. 2.Department of Chemical EngineeringUniversity of La FronteraTemucoChile
  3. 3.Department of Chemical Engineering, Environmental Biotechnology Center, Faculty of Engineering, Sciences and AdministrationUniversity of La FronteraTemucoChile

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