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Optimization and characterization of a biosensor assembly for detection of Salmonella Typhimurium

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Abstract

The performance of biosensors depends directly on the strategies adopted during their development. In this paper, a fast and sensitive biosensor for Salmonella Typhimurium detection was assembled by using optimization studies in separate stages. The pre-treatment assays, biomolecular immobilization (primary antibody and protein A concentrations), and analytical response (hydroquinone and hydrogen peroxide concentrations) were optimized via voltammetric methods. In the biosensor assembly, a gold surface was modified via the self-assembled monolayer technique (SAM) using cysteamine thiol and protein A for immobilization of anti-Salmonella antibody. The analytical response of the biosensor was obtained through the use of a secondary antibody labeled with a peroxidase enzyme, and the signal was evaluated by applying the chronoamperometry technique. The biosensor was characterized by infrared spectroscopy and cyclic voltammetry. Optimization of protein A and primary antibody concentrations enabled higher analytical signals of 7.5 and 75 mg mL−1, respectively, to be achieved. The hydroquinone and H2O2 concentrations selected were 3 and 300 mM, respectively. The biosensor developed attained a very low detection limit of 10 CFU mL−1 and a fast response with a final detection time of 125 min. These results indicate that this biosensor is very promising for the food safety and emergency response applications.

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Acknowledgements

The authors would like to thank the Brazilian agencies, CNPq, FUNCAP, and CAPES, for their financial support, Embrapa Tropical Agroindustry and National Center of Energy and Materials Research (CNPEM). Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and employer.

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Authors and Affiliations

  1. Department of Science and Food Technology, Federal University of Ceará, Campus do Pici, Bloco 858, Fortaleza, CE, 60356-000, Brazil

    A. M. A. Melo & E. A. T. Figueiredo

  2. Department of Chemistry, State University of Ceará, Av. Dr. Silas Munguba 1.700, 60, Fortaleza, CE, 740-903, Brazil

    D. L. Alexandre, M. R. F. Oliveira & C. R. Alves

  3. Embrapa Tropical Agroindustry, Rua Dra. Sara Mesquita 2270, 60, Fortaleza, CE, 511-110, Brazil

    R. F. Furtado, M. F. Borges & P. R. V. Ribeiro

  4. USDA Agricultural Research Service, National Center for Agricultural Utilization Research, 1815 North University Street, Peoria, IL, 61604, USA

    A. Biswas

  5. USDA Agricultural Research Service, Southern Regional Research Center, 1100 Robert E. Lee Blvd, New Orleans, LA, 70124, USA

    H. N. Cheng

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  1. A. M. A. Melo
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  3. M. R. F. Oliveira
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  4. R. F. Furtado
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  8. H. N. Cheng
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Correspondence to A. M. A. Melo.

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Melo, A.M.A., Alexandre, D.L., Oliveira, M.R.F. et al. Optimization and characterization of a biosensor assembly for detection of Salmonella Typhimurium. J Solid State Electrochem 22, 1321–1330 (2018). https://doi.org/10.1007/s10008-017-3767-0

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  • DOI: https://doi.org/10.1007/s10008-017-3767-0

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